Effects of Different Starch Source Diets on Small Intestinal Digestive Enzyme Activities, Caecum Volatile Fatty Acid Contents and Microbial Community of Broilers

  • WANG Xuan ,
  • WANG Dandan ,
  • NIAN Fang ,
  • TANG Defu ,
  • WANG Weimin ,
  • MA Zhiyuan ,
  • DU Baolong
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  • 1. College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China;
    2. School of Science, Gansu Agricultural University, Lanzhou 730070, China;
    3. State Key Laboratory of Grassland Agro-Ecosystems, Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, China

Received date: 2020-06-30

  Online published: 2021-01-18

Abstract

This experiment was conducted to study the effects of different starch source diets on small intestinal digestive enzyme activities, caecum volatile fatty acid contents and microbial community of broilers. A total of 480 one-day-old healthy Arbor Acres (AA) broilers were randomly divided into 5 groups with 8 replicates in each group and 12 broilers in each replicate. Broilers in 5 groups were fed semi homozygous equal nitrogen and equal energy diets prepared with corn starch, pea starch, tapioca starch, sorghum starch and wheat starch, respectively. The total starch level of 5 groups was the same. The experiment lasted for 21 days. The results showed as follows: 1) compared with corn starch group, tapioca starch group and wheat starch group, the contents of acetic acid, propionic acid and total volatile fatty acid in caecum chyme of broilers of pea starch group were significantly increased (P<0.05), and the content of total volatile fatty acid in caecum chyme of broilers of sorghum starch group was significantly increased (P<0.05). 2) The activity of amylase in jejunum chyme of broilers of sorghum starch group was significantly higher than that of other groups (P<0.05), and the activity of lipase in jejunum and ileum chyme and activities of amylase and lipase in jejunum mucosa of broilers of tapioca starch group were significantly higher than those of other groups (P<0.05). 3) The Bacteroidetes, Firmicutes, Proteobacteria and Tenericutes were the dominant phyla in cecum of broilers. Compared with wheat starch group, pea starch group and sorghum starch group, the Firmicutes relative abundance of tapioca starch group and corn starch group was significantly increased (P<0.05). Compared with corn starch group, sorghum starch group and wheat starch group, the relative abundances of Bacteroidetes, Tenericutes, Bacteroides and Odoribacter of pea starch group were significantly increased (P<0.05), and the relative abundances of Proteobacteria, Melainabacteria unidentified Enterobacteriaceae, Enterococcus and Parabacteroides were significantly decreased (P<0.05). In conclusion, the source of starch can affect the activity of digestive enzymes in small intestine, alter the cecum fermentation patterns and microbial composition of broilers, the diets of pea starch, tapioca starch and corn starch can increase the acidogenic microbes in cecum of broilers, promote microbial fermentation, change the structure of intestinal flora, and promote the growth of beneficial bacteria.

Cite this article

WANG Xuan , WANG Dandan , NIAN Fang , TANG Defu , WANG Weimin , MA Zhiyuan , DU Baolong . Effects of Different Starch Source Diets on Small Intestinal Digestive Enzyme Activities, Caecum Volatile Fatty Acid Contents and Microbial Community of Broilers[J]. Chinese Journal of Animal Nutrition, 2021 , 33(1) : 285 -296 . DOI: 10.3969/j.issn.1006-267x.2021.01.029

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