Effects of Artemisia Polysaccharide Combination Preparation on Rumen Microflora Diversity of Tan Lambs

JIN Lu; LI Shengli; SANG Dan; ZHANG Chongzhi; ZHANG Chunhua; ZHANG Junli; SHI An; SUN Haizhou; LI Jucai

doi:10.3969/j.issn.1006-267x.2021.01.032

Chinese Journal of Animal Nutrition >

2021 , Vol. 33 >Issue 1: 317 - 329

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.01.032

Effects of Artemisia Polysaccharide Combination Preparation on Rumen Microflora Diversity of Tan Lambs

JIN Lu ,
LI Shengli ,
SANG Dan ,
ZHANG Chongzhi ,
ZHANG Chunhua ,
ZHANG Junli ,
SHI An ,
SUN Haizhou ,
LI Jucai

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1. Inner Mongolia Key Laboratory of Herbivores Nutrition, Animal Nutrition and Feed Research Institution of Inner Mongolia Academy of Agriculture and Animal Husbandry Academy, Hohhot 010031, China;
2. Animal Sciences Institute of Ningxia Academy of Agriculture and Forestry Sciences, Yinchuan 750002, China

Received date: 2020-08-14

Online published: 2021-01-18

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Abstract

This experiment was conducted to evaluate the effects of Artemisia polysaccharide combination preparation on rumen microflora diversity of Tan lambs. Sixty healthy male Tan lambs with similar body weight were randomly divided into four groups according to body weight, each group contained 15 lambs. Lambs in the control group were fed a basal diet, and others in trial group 1, trial group 2 and trial group 3 were fed basal diets supplemented 5.5 g/d Artemisia polysaccharide+5.5 g/d coated sodium butyrate, 5.5 g/d Artemisia polysaccharide+5.5 g/d butyrate glyceride and 5.5 g/d Artemisia polysaccharide+1.1 g/d monensin, respectively. The pre-experimental period lasted for 15 days, and the experimental period lasted for 60 days. The results showed as follows: 1) compared with the control group, the concentrations of acetic acid, butyric acid and total volatile fatty acids and proportion of acetic acid in rumen fluid of Tan sheep of trial group 1 and trial group 2 were significantly increased (P<0.05). 2) The Chao1 index of trial group 2 was significantly higher than that of the control group (P<0.05), and the Shannon index and Simpson index of trial group 2 were trend higher than the control group (0.05≤P<0.10). 3) At phylum level, compared with the control group, the relative abundance of Tenericutes in rumen fluid of trial group 1 was significant increased (P<0.05), and the relative abundances of Firmicutes and Fibrobacteres were trend to increase (0.05≤P<0.10); the relative abundance of Chloroflexi in rumen fluid of trial group 2 was significant increased (P<0.05), and the relative abundance of Verrucomicrobia was trend to increase (0.05≤P<0.10); the relative abundances of Cyanobacteria and Synergistetes in rumen fluid were significant increased (P<0.05). 4) At genus level, compared with the control group, the relative abundance of Rikenellaceae_RC9_gut_group in rumen fluid of trial group 1 was significant decreased (P<0.05), and the relative abundances of Succiniclasticum and Solobacterium were significant increased (P<0.05); the relative abundance of Sharpea in rumen fluid of trial group 2 was significant decreased (P<0.05), and the relative abundance of Pseudobutyrivibrio was significant increased (P<0.05); the relative abundances of Rikenellaceae_RC9_gut_group and Sharpea in rumen fluid of trial group 3 were significant decreased (P<0.05), and the relative abundance of Syntrophococcus was trend to increase (0.05≤P<0.10). In conclusion, dietary Artemisia polysaccharide combination preparation can increase the microflora diversity and improve the rumen microflora of Tan lambs, and the effect of adding Artemisia polysaccharide+coated sodium butyrate is the best.

Key words： Artemisia polysaccharides; Tan lambs; microflora diversity

Cite this article

JIN Lu , LI Shengli , SANG Dan , ZHANG Chongzhi , ZHANG Chunhua , ZHANG Junli , SHI An , SUN Haizhou , LI Jucai . Effects of Artemisia Polysaccharide Combination Preparation on Rumen Microflora Diversity of Tan Lambs[J]. Chinese Journal of Animal Nutrition, 2021 , 33(1) : 317 -329 . DOI: 10.3969/j.issn.1006-267x.2021.01.032

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