Effects of Grape Seed Proanthocyanidins on Immune Function of High-Concentrate Fattening Lambs

  • MU Chuntang ,
  • YANG Wenjun ,
  • WANG Pengju ,
  • REN Guodong ,
  • JU Hao ,
  • ZHANG Xuanzi ,
  • ZHANG Jianxin ,
  • HAO Xiaoyan
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  • College of Animal Sciences, Shanxi Agricultural University, Jinzhong 030801, China

Received date: 2020-06-24

  Online published: 2021-01-18

Abstract

The aim of this experiment was to investigate the effects grape seed proanthocyanidins (GSPs) on immune function of high-concentrate fattening lambs. Forty-eight 4-month-old Dorper ( )×small tail Han sheep (♀) F1 male lambs with similarly weight of (22.75±1.20) kg were randomly selected and randomly divided into 4 groups with 12 lambs per group. Lambs in 4 groups were fed basic diets supplemented 0 (control group), 10 (10GSPs group), 20 (20GSPs group) and 40 mg/(kg BW·d) (40GSPs group) GSPs, respectively. The pre-experimental period lasted for 15 days, and the experimental period lasted for 45 days. The results showed as follows: 1) on day 30 of the trial, the proportion of CD4+ cells in peripheral blood of 20GSPs and 40GSPs groups was significantly higher than that of the control group (P<0.05), and the proportion of CD8+ cells was significantly lower than that of the control group (P<0.05). On day 45 of the trial, the proportion of CD8+ cells in peripheral blood of 20GSPs and 40GSPs groups was significantly lower than that of the control group (P<0.05), and the proportion of CD4+ cells and CD4+/CD8+ ratio were significantly higher than those of the control group (P<0.05). 2) With the GSPs supplemental level increased, the peripheral blood lymphocyte transformation ratio linearly increased (P<0.05), while the lymphocyte apoptosis ratio linearly decreased (P<0.05). The peripheral blood lymphocyte transformation ratio of 20GSPs and 40GSPs groups was significantly higher than that of the control group (P<0.05), while the lymphocyte apoptosis ratio was significantly lower than that of the control group (P<0.05). 3) With the GSPs supplemental level increased, the serum interleukin-2 (IL-2) level showed a linear increase trend (P=0.057), the serum interleukin-6 (IL-6) showed a linear decrease trend (P=0.074), but there was no significant difference on contents of IL-2 and IL-6 in serum among all groups (P>0.05). With the GSPs supplemental level increased, the serum immunoglobulin G (IgG) content linearly increased (P<0.05), and the serum IgG content of 20GSPs group was significantly higher than that of 10GSPs and control groups (P<0.05). In summary, the appropriate supplementation of GSPs in high-concentrate diet can improve the immune function of lambs, the optimal supplemental level of GSPs is 20 mg/(kgBW·d) under the condition of this experiment.

Cite this article

MU Chuntang , YANG Wenjun , WANG Pengju , REN Guodong , JU Hao , ZHANG Xuanzi , ZHANG Jianxin , HAO Xiaoyan . Effects of Grape Seed Proanthocyanidins on Immune Function of High-Concentrate Fattening Lambs[J]. Chinese Journal of Animal Nutrition, 2021 , 33(1) : 330 -340 . DOI: 10.3969/j.issn.1006-267x.2021.01.033

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