Storage Stability of Furosine Standard Solution and Effects of Hydrochloric Acid Concentration Used for Milk Hydrolysis Procedure on Furosine Detection Result

CHEN Meixia; CHEN Chongchong; WEN Fang; ZHANG Yangdong; ZHENG Nan; WANG Jiaqi

doi:10.3969/j.issn.1006-267x.2021.01.034

2021 , Vol. 33 >Issue 1: 341 - 349

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.01.034

Storage Stability of Furosine Standard Solution and Effects of Hydrochloric Acid Concentration Used for Milk Hydrolysis Procedure on Furosine Detection Result

CHEN Meixia ,
CHEN Chongchong ,
WEN Fang ,
ZHANG Yangdong ,
ZHENG Nan ,
WANG Jiaqi

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1. Key Laboratory of Quality & Safety Control for Milk and Dairy Products of Ministry of Agriculture and Rural Affairs, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
2. Laboratory of Quality and Safety Risk Assessment for Dairy Products of Ministry of Agriculture and Rural Affairs(Beijing), Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
3. Milk and Milk Products Inspection Center of Ministry of Agriculture and Rural Affairs(Beijing), Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
4. State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China

Received date: 2020-05-20

Online published: 2021-01-18

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Abstract

This experiment was to investigate the storage stability of furosine and the effects of hydrochloric acid (HCl) concentration used for milk hydrolysis procedure on furosine detection result. In experiment 1, in order to investigate the stability of furosine standard solution under different storage conditions, different concentrations of furosine standard solutions (1 and 5 mg/L) were prepared with different HCl solutions (3.0, 1.0 and 0.1 mol/L), and kept at different temperatures [-20, 4 ℃ and indoor temperature (25 ℃)] for different time (1, 3, 5, 8, 10, 12, 12, 15, 17 and 21 days, n=3 for each treatment), and then determined the furosine content using ultra performance liquid chromatography (UPLC) method. In experiment 2, in order to investigate the effects of HCl concentration used for milk hydrolysis procedure on furosine detection result and the repeatability of detection result, eleven different concentrations of HCl were used to hydrolyze the milk for furosine content determination. Four samples were used in each concentration, and three independent experiments were conducted separately. The results showed as follows: 1) furosine standard solutions prepared with 0.1 and 1.0 mol/L HCl degraded easily, but the furosine standard solutions prepared with 3.0 mol/L HCl exhibited good stability when they were stored at -20, 4 ℃ or room temperature (25 ℃) within 21 days. 2) HCl concentration significantly affected the hydrolysis effect of milk (P<0.05), and the milk could be completed hydrolyzed to release the furosine in milk and presented good repeatability when the concentration of HCl in the hydrolysate was up to 9.17 mol/L. Therefore, it is suggests that furosine standard solution should be prepared with 3 mol/L HCl and stored at 4 ℃ within 21 days. In addition, the concentration of HCl used in milk hydrolysis procedure should be such that can make the concentration of HCl in the hydrolysate reaches at least 9.17 mol/L.

Key words： milk; furosine; stability; hydrochloric acid; hydrolysis effect; repeatability

Cite this article

CHEN Meixia , CHEN Chongchong , WEN Fang , ZHANG Yangdong , ZHENG Nan , WANG Jiaqi . Storage Stability of Furosine Standard Solution and Effects of Hydrochloric Acid Concentration Used for Milk Hydrolysis Procedure on Furosine Detection Result[J]. Chinese Journal of Animal Nutrition, 2021 , 33(1) : 341 -349 . DOI: 10.3969/j.issn.1006-267x.2021.01.034

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