Effects of Different Zinc Sources on Humoral Immunity of Goats and Kid Goats and Intestinal Mucosal Morphology, Immune of Kid Goats

TIAN Lina; YAN Qiongxian; ZHENG Mengli; ZHOU Chuanshe; TAN Zhiliang

doi:10.3969/j.issn.1006-267x.2021.01.036

2021 , Vol. 33 >Issue 1: 359 - 369

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.01.036

Effects of Different Zinc Sources on Humoral Immunity of Goats and Kid Goats and Intestinal Mucosal Morphology, Immune of Kid Goats

TIAN Lina ,
YAN Qiongxian ,
ZHENG Mengli ,
ZHOU Chuanshe ,
TAN Zhiliang

Expand

1. Institute of Subtropical Agriculture, Chinese Academy of Sciences, Key Laboratory of Agro-Ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production, Hunan Provincial Key Laboratory of Nutritional Physiology and Metabolic Process, Changsha 410125, China;
2. University of Chinese Academy of Sciences, Beijing 100094, China;
3. Hunan Co-Innovation Center for Production Safety, Changsha 410128, China

Received date: 2020-05-27

Online published: 2021-01-18

Fold

Abstract

This experiment was conducted to study the effects of dietary different zinc sources of pregnant goats on humoral immunity of goats and kid goats and intestinal morphology, mucosal immune function of kid goats. Twenty-one Xiangdong black goats with twin pregnancies, similar parity (the 2 to 3 fetus) and body weight [(38.1±9.7) kg] were randomly divided into 3 groups with 7 replicates per group and 1 goat per replicate. Goats in 3 groups were fed basal diets supplemented with 60 mg/kg zinc sulfate, methionine chelating zinc (Zn-Met) and glycine chelating zinc (Zn-Gly), respectively. The zinc content of the basal diet was 22 mg/kg, and the zinc content of each experimental diet was 82 mg/kg. The pre-experimental period lasted for 7 days, and the experimental period lasted for 45 days. The jugular veinal blood of pregnant goats at day 10 before birth, and newborn goats at days 30, 60 and 90 was collected, and measured the contents of immunoglobulins (Ig) and cytokines in plasma of goats and kid goats. The kid goats were slaughtered at day 100 and the intestinal tissue were collected to determine the intestinal morphology and contents of Ig and cytokines in intestinal mucosa. The results showed as follows: 1) on day 10 of goats before delivery, the contents of interleukin (IL)-6 and IL-8 in plasma of Zn-Gly group were significantly higher than those of zinc sulfate group and Zn-Met group (P<0.05). 2) On day 30 after kid goats' birth, the plasma IgG content of Zn-Met group was significantly higher than that of Zn-Gly group (P<0.05). On day 60 after kid goats birth, the plasma IL-4 content of zinc sulfate group and Zn-Met group was significantly higher than that of Zn-Gly group (P<0.05), the contents of IL-6 and IL-22 in plasma of Zn-Met group were significantly higher than those of zinc sulfate group and Zn-Gly group (P<0.05), and the plasma IgG content of Zn-Gly group was significantly higher than that of sulfate group and Zn-Met group (P<0.05). On day 100 after kid goats birth, there were no significant differences on contents of cytokines and Ig in plasma among all groups (P>0.05). 3) There were no significant differences on villus width, villus height, crypt depth and villus height/crypt depth in jejunum and ileum among all groups (P>0.05). 4) The contents of IL-6, IL-22, IL-23 and IgG in jejunal mucosa of Zn-Met group and Zn-Gly group were significantly higher than those of zinc sulfate group (P<0.05), the jejunal mucosa IL-4 content of Zn-Gly group was significantly higher than that of zinc sulfate group and Zn-Met group (P<0.05), and the jejunal mucosa secretory immunoglobulin A (sIgA) content of Zn-Met group was significantly higher than that of zinc sulfate group and Zn-Gly group (P<0.05). The contents of IL-4, IL-6, IL-23, IgM and IgG in ileal mucosa of Zn-Met group and Zn-Gly group were significantly higher than those of zinc sulfate group (P<0.05), the ileal mucosa sIgA content of Zn-Met group was significantly higher than that of zinc sulfate group and Zn-Gly group (P<0.05), and the contents of IgM and sIgA in ileal mucosa of Zn-Met group were significantly higher than those of Zn-Gly group (P<0.05). In conclusion, dietary supplementation of Zn-Met and Zn-Gly for pregnant goats can improve the humoral immunity and intestinal mucosal immune function of kid goats, and the Zn-Met is better than Zn-Gly.

Key words： amino acid chelating zinc; kid goats; immunoglobulins; cytokines

Cite this article

TIAN Lina , YAN Qiongxian , ZHENG Mengli , ZHOU Chuanshe , TAN Zhiliang . Effects of Different Zinc Sources on Humoral Immunity of Goats and Kid Goats and Intestinal Mucosal Morphology, Immune of Kid Goats[J]. Chinese Journal of Animal Nutrition, 2021 , 33(1) : 359 -369 . DOI: 10.3969/j.issn.1006-267x.2021.01.036

References

[1] HE Y,YUAN X M,ZUO H,et al.Berberine induces ZIP14 expression and modulates zinc redistribution to protect intestinal mucosal barrier during polymicrobial sepsis[J].Life Sciences,2019,5(4):496-513.
[2] SHANNON M C,HILL G M.Trace mineral supplementation for the intestinal health of young monogastric animals[J].Frontiers in Veterinary Science,2019,6(73):1-7.
[3] SKROVANEK S,DIGUILIO K,BAILEY R,et al.Zinc and gastrointestinal disease[J].World Journal of Gastrointestinal Pathophysiology,2014,5(4):496-513.

[4] PRASAD A S.Discovery of human zinc deficiency:its impact on human health and disease[J].Advances in Nutrition,2013,4(2):176-190.

[5] GARG A K,MUDGAL V,DASS R S.Effect of organic zinc supplementation on growth,nutrient utilization and mineral profile in lambs[J].Animal Feed Science and Technology,2008,144(1/2):82-96.
[6] WANG R L,LIANG J G,LU L,et al.Effect of zinc source on performance,zinc status,immune response,and rumen fermentation of lactating cows[J].Biological Trace Element Research,2013,152(1):16-24.

[7] SETHY K,BEHERA K,MISHRA S K,et al.Effect of organic zinc supplementation on growth,metabolic profile and antioxidant status of Ganjam sheep[J].Indian Journal of Animal Research,2018,52(6):839-842.
[8] CAO J,HENRY P R,GUO R,et al.Chemical characteristics and relative bioavailability of supplemental organic zinc sources for poultry and ruminants[J].Journal of Animal Science,2000,78(8):2039-2054.

[9] GLOVER A D,PUSCHNER B,ROSSOW H A,et al.A double-blind block randomized clinical trial on the effect of zinc as a treatment for diarrhea in neonatal Holstein calves under natural challenge conditions[J].Preventive Veterinary Medicine,2013,112(3/4):338-347.
[10] NAGALAKSHMI D,DHANALAKSHMI K,HIMABINDU D.Effect of dose and source of supplemental zinc on immune response and oxidative enzymes in lambs[J].Veterinary Research Communications,2009,33(7):631-644.

[11] MANDAL G P,DASS R S,ISORE D P,et al.Effect of zinc supplementation from two sources on growth,nutrient utilization and immune response in male crossbred cattle (Bos indicus×Bos taurus) bulls[J].Animal Feed Science and Technology,2007,138(1):1-12.
[12] METZLER-ZEBELI B U,CAINE W R,MCFALL M,et al.Supplementation of diets for lactating sows with zinc amino acid complex and gastric nutriment-intubation of suckling pigs with zinc methionine on mineral status,intestinal morphology and bacterial translocation in lipopolysaccharide-challenged weaned pigs[J].Journal of Animal Physiology and Animal Nutrition,2010,94(2):237-249.

[13] MARQUES R S,COOKE R F,RODRIGUES M C,et al.Effects of organic or inorganic cobalt,copper,manganese,and zinc supplementation to late-gestating beef cows on productive and physiological responses of the offspring[J].Journal of Animal Science,2016,94(3):1215-1226.

[14] 中华人民共和国农业部.中华人民共和国农业行业标准——肉羊饲养标准(NY/T 816-2004)[J].湖南饲料,2004(6):9-15. Ministry of Agriculture of the People's Republic of China. Agricultural industry standard of the People's Republic of China——sheep feeding standard (NY/T 816-2004)[J].Hunan Feed,2004(6):9-15.(in Chinese)
[15] ENRÍQUEZ D,HÖETZEL M J,UNGERFELD R.Minimising the stress of weaning of beef calves:a review[J].Acta Veterinaria Scandinavica,2011,53(1):28.
[16] BOSWELL H S,SHARROW S O,SINGER A.Role of accessory cells in B cell activation.1.Macrophage presentation of tnp-ficoll:evidence for macrophage B cell interaction[J].Journal of Immunology,1980,124(2):989-996.
[17] ALLAIRE J M,CROWLEY S M,LAW H T,et al.The intestinal epithelium:central coordinator of mucosal immunity[J].Trends in immunology,2018,39(9):677-696.

[18] ZHANG Q,LI C,NIU X L,et al.An intensive milk replacer feeding program benefits immune response and intestinal microbiota of lambs during weaning[J].Bmc Veterinary Research,2018,14(1):366.
[19] HART P H,VITTI G F,BURGESS D R,et al.Potential antiinflammatory effects of interleukin-4 suppression of human monocyte tumor necrosis factor-alpha,interleukin-1,and prostaglandin-e2[J].Proceedings of the National Academy of Sciences of the United States of America,1989,86(10):3803-3807.

[20] CHOMARAT P,BANCHEREAU J.An update on interleukin-4 and its receptor[J].European Cytokine Network,1997,8(4):333-344.
[21] LIANG H E,REINHARDT R L,BANDO J K,et al.Divergent expression patterns of IL-4 and IL-13 define unique functions in allergic immunity[J].Nature Immunology,2012,13(1):58-66.

[22] OH C K,GEBA G P,MOLFINO N.Investigational therapeutics targeting the IL-4/IL-13/STAT-6 pathway for the treatment of asthma[J].European Respiratory Review,2010,19(115):46-54.

[23] PAYNE R L,BIDNER T D,FAKLER T M,et al.Growth and intestinal morphology of pigs from sows fed two zinc sources during gestation and lactation[J].Journal of Animal Science,2006,84(8):2141-2149.

[24] MAO K R,BAPTISTA A P,TAMOUTOUNOUR S,et al.Innate and adaptive lymphocytes sequentially shape the gut microbiota and lipid metabolism[J].Nature,2018,554(7691):255-259.

[25] UENO A,GHOSH A,HUNG D,et al.Th17 plasticity and its changes associated with inflammatory bowel disease[J].World Journal of Gastroenterology,2015,21(43):12283-12295.

[26] MALOY K J.The interleukin-23/interleukin-17 axis in intestinal inflammation[J].Journal of internal medicine,2008,263(6):584-590.

[27] KULLBERG M C,JANKOVIC D,FENG C G,et al.IL-23 plays a key role in Helicobacter hepaticus-induced T cell-dependent colitis[J].Journal of Experimental Medicine,2006,203(11):2485-2494.

[28] BRANDTZAEG P.Current understanding of gastrointestinal immunoregulation and its relation to food allergy[J].Annals of the New York Academy of Sciences,2002,964(1):13-45.
[29] MALLAKI M,NOROUZIAN M A,KHADEM A A.Effect of organic zinc supplementation on growth,nutrient utilization,and plasma zinc status in lambs[J].Turkish Journal of Veterinary and Animal Sciences,2015,39(1):75-80.
[30] SPEARS J W,SCHLEGEL P,SEAL M C,et al.Bioavailability of zinc from zinc sulfate and different organic zinc sources and their effects on ruminal volatile fatty acid proportions[J].Livestock Production Science,2004,90(2/3):211-217.
[31] 梁建光,吕林,罗绪刚.有机锌源的理化特性及其体外瘤胃发酵的稳定性研究[J].畜牧兽医学报,2008,39(10):1355-1366. LIANG J G,LYU L,LUO X G,et al.Physical and chemical characteristics of supplemental organic zinc sources and their stabilities in vitro fermentation rumen[J].Chinese Journal of Animal and Veterinary Sciences,2008,39(10):1355-1366.(in Chinese)

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article

References