T-2 Toxin Induced Inflammatory Response of Intestinal Porcine Epithelial Cells by Activating Reactive Oxygen Species/ Nuclear Factor-κB Signaling Pathway

  • KANG Ruifen ,
  • WANG Meng ,
  • SHEN Jiakun ,
  • JIN Xiaoming ,
  • LI Chunmei
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  • College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China

Received date: 2020-06-19

  Online published: 2021-01-18

Abstract

This experiment used the intestinal porcine epithelial cells (IPEC-J2) as model cells, to investigate the effects of T-2 toxin induced inflammatory response in IPEC-J2 and its related mechanism. The cell viability was measured by methyl thiazolyl tetrazolium (MTT) method, and the appropriate concentrations of T-2 toxin and N-acetyl-L-cysteine (NAC) were selected. The experiment was divided into four groups, which were control group, NAC group (4.0 mmol/L NAC), T-2 group (4.0 ng/mL T-2 toxin) and T-2+NAC group (4.0 ng/mL T-2 toxin 4.0 mmol/L NAC), respectively. After 12 h treatment, the malondialdehyde (MDA) content, total superoxide dismutase (T-SOD) activity, catalase (CAT) activity, reactive oxygen species (ROS) level and relative expression of inflammation related genes and nuclear factor-κB (NF-κB) in IPEC-J2 of each group were measured. The results showed as follows: 1) compared with the control group, the activities of CAT and T-SOD in IPEC-J2 of T-2 group were significantly decreased (P<0.01), and the MDA content and ROS level in IPEC-J2 were significantly increased (P<0.01). Compared with the T-2 group, the activities of CAT and T-SOD in IPEC-J2 of T-2+NAC group were significantly increased (P<0.01), and the MDA content and ROS level in IPEC-J2 were significantly decreased (P<0.05 or P<0.01). 2) Compared with the control group, the mRNA relative expressions of tumor necrosis factor-α (TNF-α) (P<0.05), interleukin-6 (IL-6) (P<0.01) and interleukin-10 (IL-10) (P>0.05) in IPEC-J2 of T-2 group were increased. 3) Compared with the control group, the protein relative expression of NF-κB in IPEC-J2 of T-2 group was significantly increased (P<0.01). Compared with the T-2 group, the protein relative expression of NF-κB in IPEC-J2 of T-2+NAC group was significantly decreased (P<0.01). In conclusion, T-2 toxin induce inflammatory response of IPEC-J2 by increasing ROS production and promoting expressions of inflammation related genes and NF-κB protein.

Cite this article

KANG Ruifen , WANG Meng , SHEN Jiakun , JIN Xiaoming , LI Chunmei . T-2 Toxin Induced Inflammatory Response of Intestinal Porcine Epithelial Cells by Activating Reactive Oxygen Species/ Nuclear Factor-κB Signaling Pathway[J]. Chinese Journal of Animal Nutrition, 2021 , 33(1) : 474 -483 . DOI: 10.3969/j.issn.1006-267x.2021.01.048

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