Effects of Bacillus subtilis RZ001 on Intestinal Development, Intestinal Microbiota and Expression of Wnt Signaling Pathway Related Genes of Suckling Mice

  • GUO Congcong ,
  • LI Yanru ,
  • GENG Meng ,
  • GAI Sailun ,
  • ZHANG Tengxun ,
  • QI Wei ,
  • LI Zhongyuan ,
  • SONG Yajian ,
  • LUO Xuegang ,
  • ZHANG Tongcun ,
  • WANG Nan
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  • 1. Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China;
    2. Tianjin Engineering Research Center of Microbial Metabolism and Fermentation Process Control, Tianjin 300457, China

Received date: 2020-06-24

  Online published: 2021-01-18

Abstract

This experiment was conducted to investigate the effects of early colonization of Bacillus subtilis RZ001 on growth intestinal development, intestinal microbiota and expression of Wnt signaling pathway related genes of suckling mice. Four new born C57BL/6 mice with the same number of litters were selected and divided into two groups (experimental group and control group) with two replicates in each group. From the second day after birth, the suckling mice in the experimental group were given 10 μL Bacillus subtilis RZ001 (1×107 CFU/d) by gavage for 14 continuous days, and the suckling mice in the control group were given 10 μL phosphate buffer saline by gavage for 14 continuous days. The results showed as follows: 1) compared with the control group, the body weight of suckling mice from day 2 to day 8 after birth of experimental group was significantly increased (P<0.05 or P<0.01), the weight and index of spleen, kidney and thymus were significantly increased (P<0.05 or P<0.01), but the weight and index of liver were no significant difference (P>0.05). 2) Compared with the control group, the villus density, villus length and villus length/crypt depth in small intestine of experimental group were significantly increased (P<0.05 or P<0.01), and the crypt depth was significantly decreased (P<0.05). 3) Compared with the control group, the number of goblet cells in small intestine of experimental group was significantly increased (P<0.05), and the mucin 2 (MUC2) mRNA relative expression level and MUC2 content in small intestine were significantly increased (P<0.05). 4) Compared with the control group, the mRNA relative expression levels of zonula occluden-1 (ZO-1), occludin (Ocln) and claudin-3 (Cldn-3) in small intestine of experimental group were significantly increased (P<0.01). 5) Compared with the control group, the mRNA relative expression levels of Wnt3, frizzled 7 (Fzd7), low density lipoprotein receptor related protein 5 (Lrp5), glycogen synthase kinase-3β (Gsk-3β), Ctnnb1, cellular-myelocytomatosis viral oncogene (C-myc), axin inhibitor 2 (Axin2), atonal homolog 1 (Atoh1), leucine rich repeat containing G protein-coupled receptor 5 (Lgr5) and Ki67 in small intestine of experimental group were significantly increased (P<0.05 or P<0.01), but the mRNA relative expression level of low density lipoprotein receptor related protein 6 (Lrp6) was significantly decreased (P<0.05); meanwhile, the protein relative expression level of β-catenin in small intestine of experimental group was significantly increased (P<0.05). 6) At the phylum level, compared with the control group, the relative abundance of Bacteroidetes in cecum of experimental group was significantly increased (P<0.05), while the relative abundance of Firmicutes was significantly decreased (P<0.05). At the genus level, compared with the control group, the relative abundance of Bifidobacterium in cecum of experimental group was significantly increased (P<0.05), while the relative abundance of Clostridium perfringens was significantly decreased (P<0.05). It is concluded that early application of Bacillus subtilis RZ001 can promote the growth of suckling mice, improve intestinal morphology, promote the maturation of intestinal barrier function, and thus promote early intestinal development. In addition, early application of Bacillus subtilis RZ001 can activate Wnt signaling pathway, reduce the relative abundance of enteropathogenic Clostridium perfringens, and increase the relative abundance of intestinal probiotics Bifidobacterium.

Cite this article

GUO Congcong , LI Yanru , GENG Meng , GAI Sailun , ZHANG Tengxun , QI Wei , LI Zhongyuan , SONG Yajian , LUO Xuegang , ZHANG Tongcun , WANG Nan . Effects of Bacillus subtilis RZ001 on Intestinal Development, Intestinal Microbiota and Expression of Wnt Signaling Pathway Related Genes of Suckling Mice[J]. Chinese Journal of Animal Nutrition, 2021 , 33(1) : 506 -518 . DOI: 10.3969/j.issn.1006-267x.2021.01.051

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