A Study on Intestinal Microbiota Diversity of Sows during Late Gestation and Lactation Periods

WANG Renjie; HE Yu; ZHANG Yunchang; LEI Yan; LYU Jirong; WANG Xiaolong; WU Zhenlong

doi:10.3969/j.issn.1006-267x.2021.01.054

Chinese Journal of Animal Nutrition >

2021 , Vol. 33 >Issue 1: 537 - 545

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.01.054

A Study on Intestinal Microbiota Diversity of Sows during Late Gestation and Lactation Periods

WANG Renjie ,
HE Yu ,
ZHANG Yunchang ,
LEI Yan ,
LYU Jirong ,
WANG Xiaolong ,
WU Zhenlong

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1. College of Animal Science and Technology, China Agricultural University, Beijing 100193, China;
2. Chengdu Dadhank Biotechnology Co., Ltd., Chengdu 611130, China;
3. Henan Yinfa Animal Husbandry Co., Ltd., Zhengzhou 451100, China

Received date: 2020-06-23

Online published: 2021-01-18

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Abstract

This experiment was conducted to investigate the diversity of the intestinal microbiota of sows during the late gestation and lactation periods. Six healthy Yorkshire sows (second parity) with similar backfat thickness and farrowing date were selected, and the fecal samples were collected from rectum on day 93 (G93) and day 100 (G100) of gestation, day 14 (L14) and day 21 (L21) of lactation, respectively. Microbiota diversity of fecal samples was analyzed by using Illumina HiSeq technology. The results showed as follows: 1) a total of 875 732 clean reads and 1 298 operational taxonomic units (OTU) were acquired by sequencing 24 fecal samples of 6 sows, and 1 181 and 429 OTU could be annotated at the phylum and genus levels, respectively. 2) In the aspect of alpha diversity, compared with the G93 and G100, the OTU number of L14 and L21 was significantly decreased (P<0.05); the Chao1, Observed species and Shannon indexes of L21 were significantly lower than those of G100 (P<0.05). Simpson index of L21 was lower than that of G93 (P<0.05). 3) In the aspect of beta diversity, the composition of microbiota was similar between G93 and G100, so was it between L14 and L21. However, there was a quite difference in microbiota composition between the late gestation period and the lactation period. 4) G93, G100, L14 and L21 shared 809 OTU, which could be annotated to 13 phyla and 69 genera, respectively. 5) At the phylum level, the top 3 dominant phyla were Firmicutes, Bacteroidetes and Spirochaetes, accounted for more than 93.20% of the total bacteria in each time point. The relative abundance of Euryarchaeota in L21 was significantly lower than that in G93 (P<0.05). 6) At the genus level, the top 3 dominant genera were Prevotella, Lactobacillus and Treponema. The relative abundance of Lactobacillus in L21 was significantly higher than that in G93 (P<0.05). The relative abundance of ClostridiumⅩ Ⅳa in L14 and L21 were significantly lower than that in G93 (P<0.05). In conclusion, intestinal microbiota diversity of sows during lactation period is decreased comparing with that of the sows during the late gestation. The dominant phyla are Firmicutes, Bacteroidetes and Spirochaete, and the microbiota composition is relatively stable during the late gestation and lactation periods.

Key words： sows; Illumina HiSeq sequencing technology; intestinal microbiota; diversity

Cite this article

WANG Renjie , HE Yu , ZHANG Yunchang , LEI Yan , LYU Jirong , WANG Xiaolong , WU Zhenlong . A Study on Intestinal Microbiota Diversity of Sows during Late Gestation and Lactation Periods[J]. Chinese Journal of Animal Nutrition, 2021 , 33(1) : 537 -545 . DOI: 10.3969/j.issn.1006-267x.2021.01.054

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