Effects of Dietary Supplementation with Probiotics and Synbiotics on Growth Performance and Plasma Biochemical Parameters of Huanjiang Mini-Piglets

  • TANG Wu ,
  • CAI Qiaoli ,
  • JIANG Huijiao ,
  • HUANG Pan ,
  • LIANG Shili ,
  • YU Zhongban ,
  • KONG Xiangfeng
Expand
  • 1. Key Laboratory of Agro-Ecological Processes in Subtropical Region, Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China;
    2. University of Chinese Academy of Sciences, Beijing 100049, China;
    3. Agricultural and Rural Bureau, Huanjiang Maonan Autonomous County, Huanjiang 547199, China

Received date: 2020-06-13

  Online published: 2021-01-18

Abstract

This experiment was conducted to study the effects of dietary supplementation with probiotics and synbiotics on growth performance and plasma biochemical parameters of Huanjiang mini-piglets. A total of 24 litters of newborn piglets with similar litter size were randomly divided into three groups with eight replicates per group. The piglets in control group were fed a basal diet, and the piglets in experimental groups were fed the basal diet supplemented with 0.05% Clostridium butyricum (CB, probiotics group) or 0.05% CB+0.02% xylo-oligosaccharides (synbiotics group), respectively. During 1 to 9 days of age, piglets took milk from their mother, during 10 to 35 days of age, piglets took milk from their mother and supplementary fed creep diet; during 36 to 65 days of age, piglets fed early nursery diet. During the experiment, feed intake and diarrhea were recorded daily; fasting body weight was measured at 10, 35 and 65 days of age; average daily feed intake (ADFI), average daily gain (ADG), feed/gain (F/G) and diarrhea rate were calculated at each stage; blood samples were collected at 35 and 65 days of age to determine plasma biochemical parameters. The results showed that, compared with the control group, the body weight at 65 days of age of piglets in the probiotics group and synbiotics group was significantly increased (P<0.05), the diarrhea rate was significantly decreased during 10 to 35 days of age, 36 to 65 days of age and 10 to 65 days of age (P<0.05), and the ADFI and ADG were significantly increased during 36 to 65 days of age and 10 to 65 days of age (P<0.05); at 35 days of age, the plasma total protein (TP) content and alkaline phosphatase (ALP) activity of probiotics group and symbiotics group were significantly increased (P<0.05), while the alanine transaminase (ALT) activity was significantly decreased (P<0.05); at 65 days of age, the plasma TP, albumin (ALB), triglyceride (TG) contents and ALP activity of probiotics group and synbiotics group were significantly increased (P<0.05), while the ALT and aspartate aminotransferase (AST) activities were significantly decreased (P<0.05); the low density lipoprotein-cholesterol (LDL-C) content of symbiotics group was significantly increased at 35 days of age (P<0.05), as well as the total cholesterol (TC) and LDL-C contents at 65 days of age. Compared with the probiotics group, the diarrhea rate was significantly decreased in the synbiotics group during 10 to 35 days of age, 36 to 65 days of age and 10 to 65 days of age (P<0.05); the ADFI and ADG were significantly increased in the synbiotics group during 36 to 65 days of age and 10 to 65 days of age (P<0.05); the plasma LDL-C content at 35 days of age was significantly increased in the synbiotics group (P<0.05), as well as plasma LDL-C and TC contents and ALP activity at 65 days of age. In summary, dietary supplementation with probiotics or synbiotics can increase the plasma biochemical parameters related with nitrogen metabolism and lipid metabolism, reduce the diarrhea rate, and then increase the growth performance of Huanjiang mini-piglets.

Cite this article

TANG Wu , CAI Qiaoli , JIANG Huijiao , HUANG Pan , LIANG Shili , YU Zhongban , KONG Xiangfeng . Effects of Dietary Supplementation with Probiotics and Synbiotics on Growth Performance and Plasma Biochemical Parameters of Huanjiang Mini-Piglets[J]. Chinese Journal of Animal Nutrition, 2021 , 33(1) : 546 -552 . DOI: 10.3969/j.issn.1006-267x.2021.01.055

References

[1] HUANG C,SONG P X,FAN P X,et al.Dietary sodium butyrate decreases postweaning diarrhea by modulating intestinal permeability and changing the bacterial communities in weaned piglets[J].The Journal of Nutrition,2015,145(12):2774-2780.  
[2] CAO G T,TAO F,HU Y H,et al.Positive effects of a Clostridium butyricum-based compound probiotic on growth performance,immune responses,intestinal morphology,hypothalamic neurotransmitters,and colonic microbiota in weaned piglets[J].Food & Function,2019,10(5):2926-2934.  
[3] MAIR C,PLITZNER C,DOMIG K J,et al.Original Article:impact of inulin and a multispecies probiotic formulation on performance,microbial ecology and concomitant fermentation patterns in newly weaned piglets[J].Journal of Animal Physiology and Animal Nutrition,2010,94(5):e164-e177.
[4] KONG Q,HE G Q,JIA J L,et al.Oral administration of Clostridium butyricum for modulating gastrointestinal microflora in mice[J].Current Microbiology,2011,62(2):512-517.  
[5] LI H H,LI Y P,ZHU Q,et al.Dietary supplementation with Clostridium butyricum helps to improve the intestinal barrier function of weaned piglets challenged with enterotoxigenic Escherichia coli K88[J].Journal of Applied Microbiology,2018,125(4):964-975.  
[6] 郑有秀,王超,邹晓庭,等.丁酸梭菌对断奶仔猪生长性能、肠道结构和免疫功能的影响[J].动物营养学报,2018,30(7):2683-2689. ZHENG Y X,WANG C,ZOU X T,et al.Effects of Clostridium butyricum on growth performance,intestinal structure and immune function of weaned piglets[J].Chinese Journal of Animal Nutrition,2018,30(7):2683-2689.(in Chinese)
[7] 应琳琳,张杨.木寡糖-地衣芽孢杆菌合生元对保育猪生产性能的影响[J].中国饲料,2015(21):41-43. YING L L,ZHANG Y.Effects of xylooligosaccharide-Bacillus licheniformis synbiotics on production performance of nursery pigs[J].China Feed,2015(21):41-43.(in Chinese)
[8] 姜晓明,王大会,赵献军,等.益生菌·低聚木糖制剂对仔猪生长性能和黄白痢的预防效果[J].西北农业学报,2009,18(6):59-62. JIANG X M,WANG D H,ZHAO X J,et al.The research of probiotics·xylo-oligosaccharide effect on flavo-white diarrhea precautionand growth performance of piglets[J].Acta Agriculturae Boreali-occidentalis Sinica,2009,18(6):59-62.(in Chinese)
[9] 谭兵兵.低聚木糖对断奶仔猪生长性能、血浆生化参数和肠道微生态的影响[D].硕士学位论文.南宁:广西大学,2015. TAN B B.Effects of xylo-oligosaccharide on growth performance,plasma biochemical parameters and intestinal microecology of weaned piglets[D].Master's Thesis.Nanning:Guangxi University,2015.(in Chinese)
[10] 郭秋平,文超越,王文龙,等.低聚木糖对仔猪生长性能、肌肉组织营养成分含量及肌纤维类型组成的影响[J].动物营养学报,2017,29(8):2769-2776. GUO Q P,WEN C Y,WANG W L,et al.Effects of xylooligosaccharide on growth performance,muscle nutrient content and muscle fiber type composition of piglets[J].Chinese Journal of Animal Nutrition,2017,29(8):2769-2776.(in Chinese)
[11] 邢廷杰,韩丽,解培峰,等.饲粮添加低聚木糖对肥育猪血浆生化参数和肉品质的影响[J].中国畜牧杂志,2018,54(9):94-99. XING T J,HAN L,XIE P F,et al.Effects of dietary supplementation with xylo-oligosaccharide on plasma biochemical parameters and meat quality in finishing pigs[J].Chinese Journal of Animal Science,2018,54(9):94-99.(in Chinese)
[12] 戴兆来,董红军,林勇,等.合生元组合筛选及对仔猪生产性能和腹泻的影响[J].南京农业大学学报,2008,31(2):81-85. DAI Z L,DONG H J,LIN Y,et al.Screening of lactic acid bacteria and prebiotics for synbiotics and their effects on piglets[J].Journal of Nanjing Agricultural University,2008(2):81-85.(in Chinese)
[13] WANG K L,CAO G T,ZHANG H R,et al.Effects of Clostridium butyricum and Enterococcus faecalis on growth performance,immune function,intestinal morphology,volatile fatty acids,and intestinal flora in a piglet model[J].Food & Function,2019,10(12):7844-7854.  
[14] 杨晓伟.丁酸梭菌对仔猪生长性能、肠道菌群、抗氧化功能和免疫功能的影响[J].饲料研究,2020,43(5):44-48. YANG X W. Effect of Clostridium butyricum on growth performance,intestinal flora,antioxidant function and immune function of piglets[J].Feed Research 2020,43(5):44-48.(in Chinese)
[15] 问鑫,卢萍.低聚木糖的作用机理及其在断奶仔猪生产中的应用[J].中国饲料,2018(19):24-27. WEN X,LU P.The mechanism of xylo-oligosaccharide and application in weaning piglet[J].China Feed,2018(19):24-27.(in Chinese)
[16] 邓文,焦玉萍,徐彬,等.丁酸梭菌和低聚木糖对肉鸡生产性能酸梭菌和低聚木糖对肉鸡生产性能、屠宰性能和肉品质的影响[J].中国家禽,2017,39(7):24-28. DENG W,JIAO Y P,XU B,et al.Effect of Clostridium botulinum and xylo-oligosaccharides on production performance,slaughter performance and meat quality of broilers[J].China Poultry,2017,39(7):24-28.(in Chinese)
[17] 周笑犁,汤文杰,孔祥峰,等.精氨酸对环江香猪营养物质消化率和血液指标的影响[J].华北农学报,2012,27(4):234-238. ZHOU X L,TANG W J,KONG X F,et al.Effects of arginine on nutrient digestibility and blood parameters in huanjiang mini-pigs[J].Acta Agriculturae Boreali-Sinica,2012,27(4):234-238.(in Chinese)
[18] LIU Y Y,KONG X F,JIANG G L,et al.Effects of dietary protein/energy ratio on growth performance,carcass trait,meat quality,and plasma metabolites in pigs of different genotypes[J].Journal of Animal Science and Biotechnology,2015,6(1):36.
[19] FERNANDEZ N J,Kidney B A.Alkaline phosphatase:beyond the liver[J].Veterinary Clinical Pathology,2007,36(3):223-233.  
[20] 王秋颖.碱性磷酸酶特性及其应用的研究进展[J].中国畜牧兽医,2011,38(1):157-161. WANG Q Y.Advances on characteristic and application of alkaline phosphatase[J].China Animal Husbandry & Veterinary Medicine,2011,38(1):157-161.(in Chinese)
[21] 周根来,陶勇,倪黎纲,等.日粮营养水平对育肥苏姜猪血清生化指标和肉质相关基因的影响[J].福建农业学报,2019,34(6):668-677. ZHOU G L,TAO Y,NI L G,et al.Serum biochemistry and expression of carcass trait-related gene of sujiang pigs as affected by dietary makeup at final growth stage[J].Fujian Journal of Agricultural Sciences,2019,34(6):668-677.(in Chinese)
[22] SHARMAN M J,FERNANDEZ M L,ZERN T L,et al.Replacing dietary carbohydrate with protein and fat decreases the concentrations of small LDL and the inflammatory response induced by atherogenic diets in the guinea pig[J].The Journal of Nutritional Biochemistry,2008,19(11):732-738.  
Outlines

/