Mechanism of Quercetin on Protein Availability in Porcine Intestinal Epithelial Cells

  • YAO Jiaying ,
  • MAO Yanjun ,
  • WANG Shanshan ,
  • LU Han ,
  • WANG Bo ,
  • YING Linlin ,
  • LI Yao
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  • College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China

Received date: 2020-05-17

  Online published: 2021-01-18

Abstract

This experiment was conducted to investigate the mechanism of quercetin promoting protein availability in porcine intestinal epithelial cells. The cells in experimental groups were treated with dimethyl sulfoxide (DMSO)solution containing quercetin at different supplemental levels of 0.1, 0.2, 0.4, 0.8 and 1.6 mg/L for 72 h after incubated for 48 h, and the cells in control group were treated with 0.2% DMSO solution. Thereafter, the protein content in cells was determined by bicinchoninic acid (BCA) method. The effects of quercetin on mRNA relative expression levels of mammalian target of rapamycin (mTOR) signaling pathway, amino acids and small peptide transporters using RT-qPCR. The effects of quercetin on protein expression levels of genes related to mTOR signaling pathway using Western blot. The results showed as follows: compared with control group, 1) the content of protein in porcine intestinal epithelial cells was significantly increased by adding 0.4 and 0.8 mg/L quercetin for 72 h (P<0.01); 2) 1.6 mg/L quercetin significantly increased mRNA relative expression levels of excitatory amino acid carrier 1 (EAAC1), alanine-serine-cysteine transporter 2 (ASCT2), amino acid transporter A2 (ATA2), L-type amino acid transporter 2 (LAT2), cation amino acid transporter 1 (CAT1), related to b0,+ amino acid transporter (rBAT), y+L amino acid transporter 1 (y+LAT1), y+L amino acid transporter 2 (y+LAT2) and oligopeptide transporter 1 (PepT1) in porcine intestinal epithelial cells (P<0.01); 3) 0.4 mg/L quercetin significantly decreased mRNA relative expression level of tuberous sclerosis complex 1 (TSC1) in porcine intestinal epithelial cells (P<0.01); 0.8 mg/L quercetin significantly increased mRNA relative expression levels of mTOR and ribosomal protein S6 (RPS6) (P<0.01), whereas significantly decreased that of TSC1 (P<0.01); 1.6 mg/L quercetin significantly increased mRNA relative expression levels mTOR, eukaryotic initiation factor 4E-binding protein 1 (4E-BP1), eukaryotic translation initiation factor 4E (eIF4E), eukaryotic translation initiation factor 4B (eIF4B), eukaryotic translation initiation factor 4A (eIF4A) and ribosomal protein S6 (RPS6) (P<0.01); 4) 0.1 and 1.6 mg/L quercetin significantly increased protein expression levels of mTOR, eIF4E and eIF4A in porcine intestinal epithelial cells (P<0.01), whereas significantly decreased that of 4E-BP1 (P<0.01). In conclusion, quercetin at appropriate dose increase the utilization of protein in porcine intestinal epithelial cells by regulating gene expression of amino acid transporters, small peptide transporters and genes related to mTOR signaling pathway.

Cite this article

YAO Jiaying , MAO Yanjun , WANG Shanshan , LU Han , WANG Bo , YING Linlin , LI Yao . Mechanism of Quercetin on Protein Availability in Porcine Intestinal Epithelial Cells[J]. Chinese Journal of Animal Nutrition, 2021 , 33(1) : 553 -562 . DOI: 10.3969/j.issn.1006-267x.2021.01.056

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