A Method for Rapid Detection of Concentration of Trimethylamine in Rumen in Vitro Fermentation Liquid by Gas Chromatography

ZHOU Yang; JIN Wei; XIANG Xiaoe; CHENG Yanfen; ZHU Weiyun

doi:10.3969/j.issn.1006-267x.2021.01.060

2021 , Vol. 33 >Issue 1: 594 - 600

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.01.060

A Method for Rapid Detection of Concentration of Trimethylamine in Rumen in Vitro Fermentation Liquid by Gas Chromatography

ZHOU Yang ,
JIN Wei ,
XIANG Xiaoe ,
CHENG Yanfen ,
ZHU Weiyun

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1. National Joint Research Center for Animal Digestive Tract Nutrition, Jiangsu Province Key Laboratory of Gastrointestinal Nutrition and Animal Health, Laboratory of Gastrointestinal Microbiology, Nanjing Agricultural University, Nanjing 210095, China;
2. National Experimental Teaching Center for Animal Science, Nanjing Agricultural University, Nanjing 210095, China

Received date: 2020-05-21

Online published: 2021-01-18

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Abstract

This study aimed to establish a method for rapid determination of concentration of trimethylamine (TMA) in rumen in vitro fermentation liquid by gas chromatography. The rumen in vitro fermentation experiment was set two groups: control group (n=4) and experimental group (n=4). Each fermentation bottle in the experimental group was added with 0.0414 g trimethylamine hydrochloride, and all bottles were incubated 24 h. The fermentation liquid was pretreated with 10 mol/L potassium hydroxide (KOH). TMA was separated in WME-PEG20M column and detected by flame ionization detector. A series of TMA standard solution were prepared with the fermentation medium without background TMA to prepare standard curve. The results showed that the standard curve of TMA had a good linear relationship between 0.1 to 1.0 mg/mL (R²≥0.993), the average recovery rates were 98.44% to 100.27%, and the detection limit of TMA was 0.01 mg/mL. TMA was not detectable in the fermentation liquid at 0 and 24 h in the control group. In the experimental group, the TMA concentrations were 0.65 mg/mL at 0 h and 0.12 mg/mL at 24 h, suggesting that TMA was greatly consumed by rumen microorganisms. In conclusion, the TMA detection method established in this study is simple procedure, relatively high accuracy and sensitivity, and can quickly, accurately monitor the changes of TMA concentration in the fermentation liquid. This method provides a fast and efficient detection method for the study of the TMA metabolism by rumen microorganisms.

Key words： trimethylamine; determination; rumen; in vitro fermentation; gas chromatography

Cite this article

ZHOU Yang , JIN Wei , XIANG Xiaoe , CHENG Yanfen , ZHU Weiyun . A Method for Rapid Detection of Concentration of Trimethylamine in Rumen in Vitro Fermentation Liquid by Gas Chromatography[J]. Chinese Journal of Animal Nutrition, 2021 , 33(1) : 594 -600 . DOI: 10.3969/j.issn.1006-267x.2021.01.060

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