Effects of Lysine and Other Essential Amino Acids on Growth Performance, Serum Biochemical Indexes, Organ Indexes and Intestinal Morphology of Medium-Speed Yellow-Feather Broilers during 1 to 18 Days of Age

SHI Shourong; LIU Yongqiang; ZHANG Shan; ZHU Peiji

doi:10.3969/j.issn.1006-267x.2021.02.019

2021 , Vol. 33 >Issue 2: 779 - 791

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.02.019

Effects of Lysine and Other Essential Amino Acids on Growth Performance, Serum Biochemical Indexes, Organ Indexes and Intestinal Morphology of Medium-Speed Yellow-Feather Broilers during 1 to 18 Days of Age

SHI Shourong ,
LIU Yongqiang ,
ZHANG Shan ,
ZHU Peiji

Expand

1. Poultry Institute, Chinese Academy of Agricultural Sciences, Yangzhou 225125, China;
2. Lihua Livestock Company of Jiangsu Province, Changzhou 213100, China

Received date: 2020-08-31

Online published: 2021-02-04

Supported by

Fold

Abstract

The aim of this experiment was to study the effects of lysine and other essential amino acids on growth performance, serum biochemical indexes, organ indexes and intestinal morphology of medium-speed yellow-feather broilers during 1 to 18 days of age. A total of 810 one-day-old male medium-speed yellow-feather broilers were randomly divided into 9 groups with 6 replicates per group and 15 broilers per replicate, and fed with 9 different amino acid levels of diets. Three lysine levels (0.89%, 1.05% and 1.20%) and three other essential amino acids levels (low-level: 0.66% methionine+0.58% threonine+0.16% tryptophan; medium-level: 0.78% methionine+0.68% threonine+0.19% tryptophan; high-level: 0.90% methionine+0.78% threonine+0.22% tryptophan) were designed. The experimental period was 18 days. The results showed as follows: 1) the average daily gain of 1.05% and 1.20% lysine level groups was significantly higher than that of 0.89% lysine level group (P<0.05), and the ratio of feed to gain of 1.05% lysine level group was significantly lower than that of 0.89% and 1.20% lysine level groups (P<0.05). 2) The interaction of lysine and other essential amino acids had significant effect on serum total protein (TP) content (P<0.05). The contents of TP and uric acid in serum of 1.05% lysine level group were significantly lower than those of 0.89% and 1.20% lysine level groups (P<0.05); the contents of cholesterol and high density lipoprotein in serum of low and medium other essential amino acids level groups were significantly higher than those of high other essential amino acids level group (P<0.05), and the serum triglyceride content of low and medium other essential amino acids level groups was significantly lower than those of high other essential amino acids level group (P<0.05). 3) The interaction of lysine and other essential amino acids had significant effect on bursa of Fabricius index (P<0.05), which was highest when lysine level was 1.20%, methionine 0.78% level was, threonine level was 0.68% and tryptophan level was 0.19%. The thymus index of medium other essential amino acids level group was significantly higher than that of low and high other essential amino acids level groups (P<0.05). 4) The interaction of lysine and other essential amino acids had significant effect on jejunum crypt depth (P<0.05). The jejunum villus height of 0.89% lysine level group was significantly higher than that of 1.05% lysine level group (P<0.05). The jejunum crypt depth of low other essential amino acids level group was significantly lower than that of medium and high other essential amino acids level groups (P<0.05), and the villus height/crypt depth was significantly higher than that of medium and high other essential amino acids level groups (P<0.05). In conclusion, when the dietary levels of lysine, methionine, threonine and tryptophan are 1.05%, 0.78%, 0.68% and 0.19%, respectively, the growth performance and serum biochemical indexes of medium-speed yellow-feather broilers during 1 to 18 days of age are the best under this experimental conditions.

Key words： medium-speed yellow-feather broilers; lysine; methionine; threonine; tryptophan

Cite this article

SHI Shourong , LIU Yongqiang , ZHANG Shan , ZHU Peiji . Effects of Lysine and Other Essential Amino Acids on Growth Performance, Serum Biochemical Indexes, Organ Indexes and Intestinal Morphology of Medium-Speed Yellow-Feather Broilers during 1 to 18 Days of Age[J]. Chinese Journal of Animal Nutrition, 2021 , 33(2) : 779 -791 . DOI: 10.3969/j.issn.1006-267x.2021.02.019

References

[1] 王信喜,李小娟,张宜辉,等.家禽赖氨酸研究进展[J].中国饲料,2011(16):13-16,19. WANG X X,LI X J,ZHANG Y H,et al.Research progress of lysine in poultry[J].China Feed,2011(16):13-16.(in Chinese)
[2] 田颖,彭景,陈玉.人体赖氨酸需要量的研究进展[J].现代预防医学,2014,41(1):22-24,27. TIAN Y,PENG J,CHEN Y.Research progress of human lysine requirement[J].Modern Preventive Medicine,2014,41(1):22-24,27. (in Chinese)
[3] PLUMSTEAD P W,ROMERO-SANCHEZ H,PATON N D,et al.Effects of dietary metabolizable energy and protein on early growth responses of broilers to dietary lysine[J].Poultry Science,2007,86(12):2639-2648.

[4] BAKER D H.Ideal amino acid profiles for swine and poultry and their applications in feed formulation[R].Chesterfield:Biokyowa Publ.Co.,1997:1-24.
[5] NETKE S P,SCOTT H M,ALLEE G L.Effect of excess amino acids on the utilization of the first limiting amino acid in chick diets[J].The Journal of Nutrition,1969,99(1):75-81.

[6] BOOMGAARDT J,BAKER D H.Sequence of limiting amino acids in gelatin for the growing chick[J].Poultry Science,1972,51(5):1650-1655.

[7] BAGHEL R P S,PRADHAN K.Effect of energy,protein and limiting amino acid levels on retention of lysine,methionine and cystine and weight gains in broilers during hot season[J].Indian Journal of Animal Nutrition,1988,5(2):127-132.
[8] 席鹏彬,林映才,郑春田,等.饲粮色氨酸水平对1-21日龄黄羽肉鸡生长、体成分沉积及下丘脑5羟色胺的影响[J].动物营养学报,2011,23(1):43-52. XI P B,LIN Y C,ZHENG C T,et al.Effects of dietary tryptophan levels on growth,whole-body nutrient retention and hypothalamic serotonin concentration in yellow-feathered broilers aged 1 to 21 days[J].Chinese Journal of Animal Nutrition,2011,23(1):43-52. (in Chinese)
[9] 林厦菁,蒋守群,丁发源,等.1-21日龄快大型黄羽肉鸡低蛋白质饲粮氨基酸平衡模式[J].动物营养学报,2014,26(9):2542-2552. LIN X J,JIANG S Q,DING F Y,et al.Amino acid balance pattern of a low protein diet in fast-growing yellow-feathered broilers aged from 1 to 21 days[J].Chinese Journal of Animal Nutrition,2014,26(9):2542-2552. (in Chinese)
[10] POWELL S,BIDNER T D,SOUTHERN L L.The interactive effects of glycine,total sulfur amino acids,and lysine supplementation to corn-soybean meal diets on growth performance and serum uric acid and urea concentrations in broilers[J].Poultry Science,2009,88(7):1407-1412.

[11] ZHAI W,PEEBLES E D,WANG X,et al.Effects of dietary lysine and methionine supplementation on Ross 708 male broilers from 21 to 42 d of age (Ⅲ):serum metabolites,hormones,and their relationship with growth performance[J].Journal of Applied Poultry Research,2016,25(2):223-231.

[12] DAŞKIRAN M,ÖNOL A G,CENGIZ Ö,et al.Effects of dietary methionine levels and L-carnitine supplementation on performance and egg quality parameters of layers[J].Journal of Animal and Feed Sciences,2009,18(4):650-661.

[13] KALINOWSKI A,MORAN E T,Jr,WYATT C L.Methionine and cystine requirements of slow- and fast-feathering broiler males from three to six weeks of age[J].Poultry Science,2003,82(9):1428-1437.

[14] SI J,FRITTS C A,WALDROUP P W,et al.Effects of excess methionine from meeting needs for total sulfur amino acids on utilization of diets low in crude protein by broiler chicks[J].Journal of Applied Poultry Research,2004,13(4):579-587.

[15] CHAMRUSPOLLERT M,PESTI G M,BAKALLI R I.Influence of temperature on the arginine and methionine requirements of young broiler chicks[J].Journal of Applied Poultry Research,2004,13(4):628-638.

[16] TAGHINEJAD-ROUDBANEH M,BABAEE M J,AFROOZIYEH M,et al.Estimation of dietary threonine requirement for growth and immune responses of broilers[J].Journal of Applied Animal Research,2013,41(4):474-483.

[17] CORZO A,MORAN E J,Jr,HOEHLER D,et al.Dietary tryptophan need of broiler males from forty-two to fifty-six days of age[J].Poultry Science,2005,84(2):226-231.

[18] SHAN A S,STERLING K G,PESTI G M,et al.The influence of temperature on the threonine and tryptophan requirements of young broiler chicks[J].Poultry Science,2003,82(7):1154-1162.

[19] KNOWLES T A,SOUTHERN L L.The lysine requirement and ratio of total sulfur amino acids to lysine for chicks fed adequate or inadequate lysine[J].Poultry Science,1998,77(4):564-569.

[20] BERNAL L E P,TAVERNARI F C,ROSTAGNO H S,et al.Digestible lysine requirements of broilers[J].Brazilian Journal of Poultry Science,2014,16(1):49-54.

[21] 陈继兰,侯水生,吕连山,等.石岐黄肉鸡前期日粮总含硫氨基酸和赖氨酸需要量的研究[J].畜牧兽医学报,1997,28(5):394-400. CHEN J L,HOU S S,LV L S,et al.Study on the dietary requirements of total sulphur containing amind acid and lysine of Shiqi yellow broiler during the early growth period[J].Acta Veterinaria et Zootechnica Sinica,1997,28(5):394-400. (in Chinese)
[22] 陈盼盼,闵育娜,王哲鹏,等.日粮赖氨酸水平对1-21日龄肉鸡生长性能和血清生化指标的影响[J].中国家禽,2017,39(9):29-34. CHEN P P,MIN Y N,WANG Z P,et al.Effects of dietary lysine level on growth performance and serum biochemical parameters of broilers at the age of 1 to 21 days[J].China Poultry,2017,39(9):29-34. (in Chinese)
[23] 田河,杨桂芹,刘显军,等.以可消化氨基酸为基础配制日粮对蛋用种母鸡繁殖性能和蛋白质代谢的影响[J].中国饲料,2006(11):22-24,30. TIAN H,YANG G Q,LIU X J,et al.Effects of the diets based on digestive amino acids on reproductive ability and protein metabolism of laying hens[J].China Feed,2006(11):22-24,30. (in Chinese)
[24] GRISONI M,UZU G,LARBIER M,et al.Effect of dietary lysine level on lipogenesis in broilers[J].Reproduction Nutrition Development,1991,31(6):683-690.

[25] 孙丰.日粮精氨酸和赖氨酸水平对肉仔鸡生产性能及血液生化指标的影响[D].硕士学位论文.北京:中国农业科学院,2010. SUN F.Effects of dietary arginine and lysine on growth and blood biochemical indicator in broilers[D].Master’s Thesis.Beijing:Chinese Academy of Agricultural Sciences,2010. (in Chinese)
[26] DEBNATH B C,BISWAS P,ROY B.The effects of supplemental threonine on performance,carcass characteristics,immune response and gut health of broilers in subtropics during pre-starter and starter period[J].Journal of Animal Physiology and Animal Nutrition,2019,103(1):29-40.

[27] 王纪亭,孙存孝,杨在宾,等.肉鸡日粮中赖氨酸与精氨酸适宜比例的研究[J].畜牧兽医学报,1999,30(3):3-5. WANG J T,SUN C X,YANG Z B,et al.Study on effects of different ratios of lysine to arginine (L/A) in diets of broilers[J].Acta Veterinaria et Zootechnica Sinica,1999,30(3):3-5. (in Chinese)
[28] SCHUTTE J B,PACK M.Sulfur amino acid requirement of broiler chicks from fourteen to thirty-eight days of age.1.Performance and carcass yield[J].Poultry Science,1995,74(3):480-487.

[29] 田大龙,陈盼盼,訾宝兵,等.饲粮赖氨酸水平对22-42日龄AA肉鸡生长性能及血清生化指标的影响[J].中国家禽,2017,39(8):15-19. TIAN D L,CHEN P P,ZI B B,et al.Effects of dietary lysine level on growth performance and serum biochemical parameters of AA broilers at the age of 22 to 42 days[J].China Poultry,2017,39(8):15-19. (in Chinese)
[30] 王自力,鲁琳,朱晓宇,等.中药复方对鸡免疫器官指数及IL-2表达的动态影响[J].中国兽医科学,2007,37(6):543-546. WANG Z L,LU L,ZHU X Y,et al.Effects of Chinese materia medica on the indexes of immune organs and expression of IL-2 in immune organs in chicken[J].Veterinary Science in China,2007,37(6):543-546. (in Chinese)
[31] 李燕蒙,田大龙,陈盼盼,等.赖氨酸对肉鸡生长性能、免疫器官发育及免疫相关基因表达的影响[J].中国家禽,2019,41(4):22-27. LI Y M,TIAN D L,CHEN P P,et al.Effects of dietary lysine on growth performance,immune organs development and expression of immune-associated genes of broilers[J].China Poultry,2019,41(4):22-27. (in Chinese)
[32] 郑辉,李兵,范伟,等.两种蛋氨酸添加剂对肉仔鸡生产性能和免疫器官发育的影响[J].饲料广角,2008(1):25-27. ZHENG H,LI B,FAN W,et al.The effect of two methionines additives to the growth performance and immune organ growth of broilers[J].Feed China,2008,(1):25-27. (in Chinese)
[33] AZZAM M M M,EL-GOGARY M R.Effects of dietary threonine levels and stocking density on the performance,metabolic status and immunity of broiler chickens[J].Asian Journal of Animal and Veterinary Advances,2015,10(5):215-225.

[34] 王红梅,刘国华,陈玉林,等.日粮苏氨酸水平对0-3周龄肉仔鸡生长性能、血清生化指标及免疫机能的影响[J].中国家禽,2005,27(20):12-15. WANG H M,LIU G H,CHEN Y L,et al.Effect of dietary threonine levels on growth performance,serum biochemical parameters and immune function in broilers at 0-3 weeks[J].China Poultry,2005,27(20):12-15. (in Chinese)
[35] PAIVA D,WALK C,MCELROY A.Dietary calcium,phosphorus,and phytase effects on bird performance,intestinal morphology,mineral digestibility,and bone ash during a natural necrotic enteritis episode[J].Poultry Science,2014,93(11):2752-2762.

[36] THOMAS D V,RAVINDRAN V.Effect of cereal type on the performance,gastrointestinal tract development and intestinal morphology of the newly hatched broiler chick[J].The Journal of Poultry Science,2008,45(1):46-50.

[37] 蒋桂韬,林谦,张旭,等.日粮赖氨酸水平对12-18周龄雌性临武鸭生长性能及肠道结构的影响[J].家畜生态学报,2014,35(11):33-36. JIANG G T,LIN Q,ZHANG X,et al.Effect of diet lys level on growth performance and intestinal villi structure of 12 to 18 weeks female Linwu ducks[J].Acta Ecologae Animalis Domastici,2014,35(11):33-36. (in Chinese)
[38] 杨麒.日粮赖氨酸水平对0-3周龄肉仔鸡生长性能及肠道发育的影响[D].硕士学位论文.哈尔滨:东北农业大学,2018. YANG L.Effects of dietary lysine levels on growth performance and intestinal development of 0 to 3 weeks old broilers[D].Master’s Thesis.Harbin:Northeast Agricultural University,2018. (in Chinese)
[39] 陈娜娜,马莲香,侯川川,等.蛋氨酸锌对蛋鸡生产性能、肠道形态、组织学结构及盲肠微生物菌群的影响[J].中国畜牧杂志,2017,53(9):102-108. CHEN N N,MA L X,HOU C C,et al.Effects of zinc-methionine on performance,intestinal morphology,histological structure and cecal microbial flora of laying hens[J].Chinese Journal of Animal Science,2017,53(9):102-108. (in Chinese)
[40] KATO Y,YU D H,SCHWARTZ M Z.Glucagonlike peptide-2 enhances small intestinal absorptive function and mucosal mass in vivo[J].Journal of Pediatric Surgery,1999,34(1):18-21.

[41] EWTUSHIK A L,BERTOLO R F P,BALL R O.Intestinal development of early-weaned piglets receiving diets supplemented with selected amino acids or polyamines[J].Canadian Journal of Animal Science,2000,80(4):653-662.

[42] SELLE P H,RAVINDRAN V,RAVINDRAN G,et al.Effects of dietary lysine and microbial phytase on growth performance and nutrient utilisation of broiler chickens[J].Asian Australasian Journal of Animal Sciences,2007,20(7):1100-1107.

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article

References