Chinese Journal of Animal Nutrition >

2021 , Vol. 33 >Issue 2: 792 - 801

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.02.020

Effects of Low Protein Diet Supplemented with Zinc-Montmorillonite on Growth Performance, Immune Organ Development and Intestinal Tissue Morphology of Broilers

  • QIN Shizhen ,
  • WANG Haibo ,
  • LI Jinlu ,
  • MA Xiaodong ,
  • PAI Wengang ,
  • YANG Minmin ,
  • WU Zhenyi ,
  • CHE Yuyan ,
  • GONG Liyuan ,
  • SHI Zhaoguo
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  • College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China

Received date: 2020-07-13

  Online published: 2021-02-04

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Abstract

This experiment was conducted to investigate the effects of low protein diet supplemented with zinc-montmorillonite (Zn-MMT) on growth performance, immune organ development and intestinal tissue morphology of broilers. A total of two hundred and eighty-eight 1-day-old male Cobb broilers with similar body weight were randomly assigned into 6 groups with 6 replicates per group and 8 broilers per replicate. The broilers in the control group were fed a corn-soybean meal type low protein basal diet, those in the positive control group [zinc sulfate (ZnSO4) group] were fed the basal diet supplemented with 40 mg/kg ZnSO4, and those in the four experimental groups were fed the basal diets supplemented with 20, 40, 60 and 80 Zn-MMT (in terms of zinc content), respectively. The chickens were free to feed and drink, and the experiment lasted for 42 days. The results showed as follows: 1) compared with the control group and ZnSO4 group, low protein diets supplemented with 60 and 80 mg/kg Zn-MMT significantly reduced the average daily gain (ADG) of broilers from 1 to 21 days of age (P<0.05), and dietary 80 mg/kg Zn-MMT significantly increased the feed to gain ratio (F/G) of broilers from 1 to 21 days of age (P<0.05). Dietary Zn-MMT had no significant effects on the ADG, average daily feed intake and F/G of broilers from 22 to 42 and 1 to 42 days of age as well as the ADG of broilers from 1 to 21 days of age (P>0.05). 2) Compared with the control group, low protein diets supplemented with Zn-MMT had no significant effects on immune organ index of broilers at 21 and 42 days of age (P>0.05). While compared with the ZnSO4 group, low protein diets supplemented with Zn-MMT significantly increased the thymus index and bursa of Fabricius index of broilers at 21 days of age (P<0.05), and dietary 80 mg/kg Zn-MMT significantly increased the thymus index of broilers at 42 days of age (P<0.05). 3) Compared with the control group, low protein diets supplemented with 20 and 60 mg/kg Zn-MMT significantly increased the leg percentage of broilers at 42 days of age (P<0.05), however, compared with the ZnSO4 group, low protein diets supplemented with Zn-MMT had no significant effects on slaughter performance of broilers at 42 days of age (P>0.05). 4) Compared with the control group, low protein diets supplemented with 40 and 60 mg/kg Zn-MMT significantly reduced the crypt depth (CD) in duodenum of broilers (P<0.05), and dietary 60 mg/kg Zn-MMT significantly reduced the CD in jejunum of broilers (P<0.05); dietary 20 mg/kg Zn-MMT significantly reduced the CD in ileum of broilers and significantly increased the ratio of villus height (VH) to CD (V/C) in ileum of broilers (P<0.05); meanwhile, dietary 80 mg/kg Zn-MMT significantly reduced the V/C in jejunum of broilers (P<0.05). Compared with the ZnSO4 group, low protein diet supplemented with 20 mg/kg Zn-MMT significantly increased the V/C in ileum of broilers and significantly decreased the CD in ileum of broilers (P<0.05); dietary 40 mg/kg Zn-MMT significantly increased the VH in jejunum of broilers and significantly reduced the CD in duodenum of broilers (P<0.05); dietary 20 and 60 mg/kg Zn-MMT significantly reduced the CD in jejunum of broilers (P<0.05); at the same time, dietary 20, 40 and 60 mg/kg Zn-MMT significantly increased the V/C in jejunum of broilers (P<0.05). To sum up, low protein diets supplemented with Zn-MMT have no significant effect on the growth performance of broilers in later growth stage, but compared to the ZnSO4 supplementation, dietary Zn-MMT can significantly increase the small intestinal VH and V/C, decrease the small intestinal CD, and promote the intestine development of broilers.

Key words： znic-montmorillonite; low protein; broilers; growth performance; intestine development; immune

Cite this article

QIN Shizhen , WANG Haibo , LI Jinlu , MA Xiaodong , PAI Wengang , YANG Minmin , WU Zhenyi , CHE Yuyan , GONG Liyuan , SHI Zhaoguo . Effects of Low Protein Diet Supplemented with Zinc-Montmorillonite on Growth Performance, Immune Organ Development and Intestinal Tissue Morphology of Broilers[J]. Chinese Journal of Animal Nutrition, 2021 , 33(2) : 792 -801 . DOI: 10.3969/j.issn.1006-267x.2021.02.020

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