Effects of Coated Sodium Butyrate on Serum Inflammatory Cytokines, Intestinal Permeability and Intestinal Morphology of Weaned Lambs Challenged with Lipopolysaccharide

WANG Kexin; ZUO Lijun; CHEN Xiang; JIANG Ning; PAN Chunyuan; LI Lingyan; ZHANG Aizhong

doi:10.3969/j.issn.1006-267x.2021.02.036

2021 , Vol. 33 >Issue 2: 954 - 964

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.02.036

Effects of Coated Sodium Butyrate on Serum Inflammatory Cytokines, Intestinal Permeability and Intestinal Morphology of Weaned Lambs Challenged with Lipopolysaccharide

WANG Kexin ,
ZUO Lijun ,
CHEN Xiang ,
JIANG Ning ,
PAN Chunyuan ,
LI Lingyan ,
ZHANG Aizhong

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Key Laboratory of Feed Resource Efficient Utilization and Nutrition Manipulation in Cold Region of Heilongjiang Province, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, China

Received date: 2020-07-15

Online published: 2021-02-04

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Abstract

This experiment was conducted to investigate the effects of coated sodium butyrate (CSB) on serum inflammatory cytokines, intestinal permeability and intestinal morphology in weaned lambs challenged with lipopolysaccharide (LPS). Twenty-four weaned lambs with an average body weight (11.79±0.54) kg were randomly allocated to 4 groups with 6 replicates per group and 1 lamb per replicate according to similar body weight: control group (CON), LPS group, CSB2L group and CSB3L group. CON and LPS groups were fed a basal diet, CSB2L and CSB3L groups were supplemented with 2 and 3 g/kg CSB, respectively. Three lambs in each group were slaughtered on the 28th day. LPS, CSB2L and CSB3L groups were intraperitoneally injected with 100 μg/kg BW LPS, and CON group was intraperitoneally injected with the same amount of sterile normal saline 3 h before slaughter on the 28th day. The results showed as follows: 1) the addition of CSB to the diet could significantly increase average daily feed intake (ADFI) of weaned lambs (P<0.05). The average daily gain (ADG) in CSB3L group was significantly higher than that in CON group or LPS group (P<0.05). 2) Compared with CON group, the contents of tumor necrosis factor-α (TNF-α), interleukin (IL)-1β and IL-8 in serum of weaned lambs were significantly higher in LPS group (P<0.05). The contents of serum TNF-α and IL-1β in CSB2L and CSB3L groups were significantly lower than those in LPS group (P<0.05), and the content of serum TNF-α was significantly higher than that in CON group (P<0.05). The content of serum IL-8 in CSB3L group was significantly lower than that in LPS group (P<0.05). The content of IL-10 in CSB2L and CSB3L groups was significantly higher than that in LPS group (P<0.05). 3) LPS challenge significantly increased the D-lactate (DLA) content and diamine oxidase (DAO) activity in serum of weaned lambs (P<0.05). CSB2L and CSB3L groups can significantly decreased the DLA content and DAO activity after LPS challenge (P<0.05). 4) The morphology and structure of intestinal mucosa were intact and the villi were arranged neatly in CON group. After challenge with LPS, the villi of jejunum and ileum were disorderly arranged, with different heights and swollen villi. In CSB2L and CSB3L groups, the villi of jejunum and ileum were relatively complete and neatly arranged, the degree of swelling decreased, and the epithelium was slightly exfoliated. In LPS group, villi epithelial cells were shed, glands were arranged disorderly and colonic villi fell were slightly shed. There was slight edema of cecum and colon in CSB2L group, and there was no obvious exfoliation and edema of cecal and colonic villous epithelium in CSB3L group. The villi height and villi height/crypt depth of the jejunum and ileum were significantly decreased (P<0.05). The decrease of villus height and villus height/crypt depth in jejunum and ileum were relieved in CSB2L and CSB3L groups (P<0.05). The villus height of duodenum in CSB2L and CSB3L groups was significantly higher than that in CON and LPS groups (P<0.05). Compared with CON group, ileum villi height and villi height/crypt depth were significantly increased in CSB3L group (P<0.05). It is concluded that dietary CSB can alleviate the stress of weaned lambs challenged with LPS, inhibit the increase of serum proinflammatory cytokines and intestinal permeability, then improve intestinal morphology and maintain intestinal health to a certain extent.

Key words： coated sodium butyrate; weaned lambs; lipopolysaccharide; intestinal health

Cite this article

WANG Kexin , ZUO Lijun , CHEN Xiang , JIANG Ning , PAN Chunyuan , LI Lingyan , ZHANG Aizhong . Effects of Coated Sodium Butyrate on Serum Inflammatory Cytokines, Intestinal Permeability and Intestinal Morphology of Weaned Lambs Challenged with Lipopolysaccharide[J]. Chinese Journal of Animal Nutrition, 2021 , 33(2) : 954 -964 . DOI: 10.3969/j.issn.1006-267x.2021.02.036

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