Effects of High Energy Diet on Liver miRNA Expression Profile of Laying Hens during Growing Period

  • WANG Xingguo ,
  • LU Jian ,
  • GUAN Shuyong ,
  • QU Liang ,
  • SUN Pingjiang ,
  • GUO Jun ,
  • HU Yuping ,
  • DOU Taocun ,
  • MA Meng ,
  • LI Yongfeng ,
  • WANG Kehua
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  • 1. Jiangsu Institute of Poultry Science, Yangzhou 225125, China;
    2. Nanjing Agricultural University, Nanjing 210095, China;
    3. Jiangsu Yukou Poultry Industry Co., Ltd., Suqian 223733, China

Received date: 2020-07-16

  Online published: 2021-02-04

Supported by

 

Abstract

In current study, miRNAs in liver were detected by small RNA sequencing of laying hens fed with high-energy or low-energy during the growing period, and their expression levels were analyzed. Target genes of differentially expressed miRNAs were predicted, and differentially expressed target genes were used to undergo gene ontology (GO) and kyoto encyclopedia of genes and genomes (KEGG) enrichment analysis. The results showed that compared with low energy group, the expression levels of 2 miRNAs in high energy group were up-regulated and 6 miRNAs were down-regulated (P<0.05). Forty target genes of differentially expressed miRNAs were predicted in differentially expressed genes, and the number of predicted target genes of miR-15a was the biggest for 26. Analysis of GO and KEGG of differentially expressed target genes showed that immune and regulation of molecular function were the most enriched processes, and the significantly enriched signaling pathways were related with immune and lipid metabolism. It is indicates that energy intake regulates immune and lipid metabolism of laying hens by influencing expression of miRNAs and then upregulates or downregulates expression of immune and lipid metabolism related target genes in liver, and finally affects production performance.

Cite this article

WANG Xingguo , LU Jian , GUAN Shuyong , QU Liang , SUN Pingjiang , GUO Jun , HU Yuping , DOU Taocun , MA Meng , LI Yongfeng , WANG Kehua . Effects of High Energy Diet on Liver miRNA Expression Profile of Laying Hens during Growing Period[J]. Chinese Journal of Animal Nutrition, 2021 , 33(2) : 1081 -1090 . DOI: 10.3969/j.issn.1006-267x.2021.02.048

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