Effects of Maize RNA on Fat Deposition of Mice

  • LI Meng ,
  • WEI Limin ,
  • CHEN Ting ,
  • HE Jiajian ,
  • XI Qianyun ,
  • ZHANG Jin ,
  • ZHANG Yongliang
Expand
  • 1. College of Biological Chemical Sciences and Engineering, Jiaxing University, Jiaxing 314000, China;
    2. South China Agricultural University, Guangzhou 510642, China

Received date: 2020-06-22

  Online published: 2021-02-04

Supported by

 

Abstract

The experiment was aimed to investigate the effects of maize RNA on fat deposition of mice. Twenty 28-day-old C57BL/6J mice were randomly divided into two groups. There were 10 replicates in each group, with one mouse per replicate. Normal saline (control group) and total RNA of maize (experiment group, 100 μg/d) were given by intragastric administration for 4 weeks. At the end of the experiment, growth performance, serum biochemical indexes, organ indexes, body imaging, body composition, and the relative levels of fat-related genes of the mice were detected. The results showed that the body weight, adipose tissue ratio and epididymal adipose index of the mice were reduced in the experimental group. Serum globulin (GLB), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C) and total cholesterol (TC) contents were significantly increased in the experimental group compared with the mice in the control group. Both adipogenesis genes (fatty acid synthetase and CCAAT enhancer binding protein alpha) and lipolytic genes (adipose triglyceride lipase and hormone sensitive lipase) relative mRNA expression levels in epididymal adipose tissue were significantly increased by maize RNA administration. In summary, maize RNA can promote the occurrence of fat metabolism and inhibit fat deposition. This study provides new evidences for plant-derived nucleic acids regulating fat metabolism in animals.

Cite this article

LI Meng , WEI Limin , CHEN Ting , HE Jiajian , XI Qianyun , ZHANG Jin , ZHANG Yongliang . Effects of Maize RNA on Fat Deposition of Mice[J]. Chinese Journal of Animal Nutrition, 2021 , 33(2) : 1091 -1099 . DOI: 10.3969/j.issn.1006-267x.2021.02.049

References

[1] 陈丝丝,李媛,徐可叶,等.脂肪细胞特性及其在肥胖相关炎症中的作用[J].中国细胞生物学学报,2019,41(5):979-984. CHEN S S,LI Y,XU K Y,et al.Characteristics of adipocytes and their role in obesity-related inflammation[J].Chinese Journal of Cell Biology,2019,41(5):979-984.(in Chinese)
[2] KHAN M I,JO C,TARIQ M R.Meat flavor precursors and factors influencing flavor precursors-a systematic review[J].Meat Science,2015,110:278-284.
[3] CARVER J D,WALKER W A.The role of nucleotides in human nutrition[J].The Journal of Nutritional Biochemistry,1995,6(2):58-72.  
[4] Al-OKBI S Y,MOHAMED R S,AL-SIEDY E S K,et al.Functional foods for management of diarrhea and malnutrition in rats emphasizing on nucleotides role[J].Recent Patents on Food,Nutrition & Agriculture,2020,doi:10.2174/2212798411666200410084202.
[5] 杨倬,秦文,王晶波,等.新型Foxo-1反义RNA两种给药方式的药效学、药动学和安全性观察[J].药学学报,2019,54(7):1251-1256. YANG Z,QING W,WANG J B,et al.Observation on efficacy,pharmacokinetic behaviors and safety of new modified RNA oligonucleotide targeting Foxo-1 via two routes of administration[J].Acta Pharmaceutica Sinica,2019,54(7):1251-1256.(in Chinese)
[6] LI M,CHEN T,HE J J,et al.Plant MIR167e-5p inhibits enterocyte proliferation by targeting β-catenin[J].Cells,2019,8(11):1385.
[7] LI M,CHEN T,WANG R,et al.Plant MiR156 regulates intestinal growth in mammals by targeting the Wnt/β-catenin pathway[J].American Journal of Physiology:Cell Physiology,2019,317(3):C434-C448.
[8] JANG B C.Artesunate inhibits adipogeneis in 3T3-L1 preadipocytes by reducing the expression and/or phosphorylation levels of C/EBP-α,PPAR-γ,FAS,perilipin A,and STAT-3[J].Biochemical and Biophysical Research Communications,2016,474(1):220-225.  
[9] MORAK M,SCHMIDINGER H,RIESENHUBER G,et al.Adipose triglyceride lipase (ATGL) and hormone-sensitive lipase (HSL) deficiencies affect expression of lipolytic activities in mouse adipose tissues[J].Molecular & Cellular Proteomics,2012,11(12):1777-1789.  
[10] PISTOR K E,SEPA-KISHI D M,HUNG S,et al.Lipolysis,lipogenesis,and adiposity are reduced while fatty acid oxidation is increased in visceral and subcutaneous adipocytes of endurance-trained rats[J].Adipocyte,2014,4(1):22-31.
[11] 孙成娟,许厚强,赵佳福,等.从江香猪肌内和皮下脂肪前体细胞分化过程中相关基因的表达[J].农业生物技术学报,2017,25(12):1979-1988. SUN C J,XU H Q,ZHAO J F,et al.The expression of related genes on intramuscular and subcutaneous preadipocytes during differentiation in Congjiang Xiang pig (Sus scrofa)[J].Journal of Agricultural Biotechnology,2017,25(12):1979-1988.(in Chinese)
[12] KLEIN S,KLEIN K,LUU K,et al.Effect of weight loss on whole body and cellular lipid metabolism in severely obese humans[J].The American Journal of Physiology:Endocrinology and Metabolism,1996,270(5):E739-E745.
[13] SCHOISWOHL G,STEFANOVIC-RACI M,MENKE M N,et al.Impact of reduced ATGL-mediated adipocyte lipolysis on obesity-associated insulin resistance and inflammation in male mice[J].Endocrinology,2015,156(10):3610-3624.  
[14] 苏晓明.小肠RNA对辐射损伤后肠道免疫的影响[D].硕士学位论文.西安:第四军医大学,2006. SU X M.Effects of intestinal RNA on intestinal immune after radiation damage[D].Master's Thesis.Xi'an:Gourth Military Medical University,2006.(in Chinese)
[15] ZHANG L,HOU D X,CHEN X,et al.Exogenous plant MIR168a specifically targets mammalian LDLRAP1:evidence of cross-kingdom regulation by microRNA[J].Cell Research,2012,22(1):107-126.  
[16] ZHOU Z,LI X H,LIU J X,et al.Honeysuckle-encoded atypical microRNA2911 directly targets influenza A viruses[J].Cell Research,2015,25(1):39-49.  
[17] 王胜男.食物成分和运动训练对雄性小鼠体重调节的影响及其生理生化机制[D].硕士学位论文.广西:广西师范大学,2017. WANG S N.Effects of food composition and exercise training on body weight regulation and physiological and biochemical mechanisms in male mice[D].Master's Thesis.Guangxi:Guangxi Normal University,2017.(in Chinese)
[18] SANDERSON I R,HE Y P.Nucleotide uptake and metabolism by intestinal epithelial cells[J].The Journal of Nutrition,1994,124(Suppl.1):131S-137S.
[19] ZHANG Y L,GUO H L,ZHANG C S,et al.AMP as a low-energy charge signal autonomously initiates assembly of AXIN-AMPK-LKB1 complex for AMPK activation[J].Cell Metabolism,2013,18(4):546-555.  
[20] 李解.外源核苷酸对斑马鱼脂代谢和免疫的调控和相关机理研究[D].硕士学位论文.北京:中国农业科学院,2019. LI J.The regulation of lipid metabolism and immunity of zebrafish by dietary supplemented nucleotides and the associated mechanisms.[D].Master's Thesis.Beijing:Chinese Academy of Agricultural Sciences,2019.(in Chinese)
[21] LI J J,YANG Z Y,YU B,et al.Methylation protects miRNAs and siRNAs from a 3'-end uridylation activity in Arabidopsis[J].Current Biology,2005,15(16):1501-1507.  
[22] RONG D W,SUN H D,LI Z X,et al.An emerging function of circRNA-miRNAs-mRNA axis in human diseases[J].Oncotarget,2015,8(42):73271-73281.
[23] 陈辉.猪脂肪沉积相关的MicroRNA研究进展[J].猪业科学,2019,36(6):112-115. CHEN H.Research progress of microRNA related to porcine fat deposition[J].Swine industry science,2019,36(6):112-115.(in Chinese)
[24] SUN L,GOFF L A,TRAPNELL C,et al.Long noncoding RNAs regulate adipogenesis[J].Proceedings of the National Academy of Sciences of the United States of America,2013,110(9):3387-3392.  
[25] LIANG G M,YANG Y L,NIU G L,et al.Genome-wide profiling of Sus scrofa circular RNAs across nine organs and three developmental stages[J].DNA Research,2017,24(5):523-535.  
[26] LUO Y,WANG P J,WANG X,et al.Detection of dietetically absorbed maize-derived microRNAs in pigs[J].Scientific Reports,2017,7:645.
[27] WITWER K.Alternative miRNAs? human sequences misidentified as plant miRNAs in plant studies and in human plasma[J].F1000research,2018,7:244.
Outlines

/