Difference in Two Types of Oxygen Bomb Calorimeter to Determine Gross Energy and Its Effects on Energy Digestibility of Samples

  • DU Zhongyuan ,
  • SU Yanfang ,
  • CHEN Kaixuan ,
  • ZHANG Hu ,
  • GAO Qingtao ,
  • ZHAO Feng
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  • State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China

Received date: 2020-06-28

  Online published: 2021-02-04

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Abstract

The objective of this experiment was to investigate the difference in the two types of oxygen bomb calorimeter to determine gross energy (GE) and its effects on energy digestibility of samples. A two-sample comparison experimental design was adopted to study the difference in GE and its coefficient of variation for four diets, three feed ingredients and four feces determined with two types of oxygen bomb calorimeters, as well as the difference in the enzymatic hydrolysate gross energy (EHGE) and energy digestibility calculated from the GE of diets, feed ingredients and corresponding residues after simulated digestion. In the determination of intra-day and inter-day variation, the GE of the samples were measured four times daily with once every 3 h from 09:00 to 21:00 and continued to measure for 4 d. In the determination of diet energy digestibility in the simulated digestion, each sample contained 10 replicates. The undigested residues were dried and randomly divided into 2 groups. The two types of oxygen bomb calorimeter were used to measure the GE of each diets or feed ingredients in duplicate, and the GE of each undigested residues only once. The results showed as follows: 1) there were significant differences between the GE measured by the two types of oxygen bomb calorimeter for each of diets and feed ingredients (P<0.05), while no difference was observed between the GE of feces. 2) The smaller intra-day, inter-day and the total coefficients of variation of GE for feces were observed in the oxygen bomb calorimeter type 2 compared with oxygen bomb calorimeter type 1. 3) There were significant differences in the EHGE in each of diet 3 and cottonseed meal and energy digestibilities in each of diet 3 and three feed ingredients calculated from the GE of samples and undigested residues measured by two types of oxygen bomb calorimeter (P<0.05). In conclusion, two types of oxygen bomb calorimeter present a difference in GE measurement for diets and feed ingredients, and present a great difference in the repeatability of fecal GE. Differences in GE measured by two types of oxygen bomb calorimeter lead to differences in EHGE and energy digestibility in some samples, but the extent of this difference is much less than the difference in GE.

Cite this article

DU Zhongyuan , SU Yanfang , CHEN Kaixuan , ZHANG Hu , GAO Qingtao , ZHAO Feng . Difference in Two Types of Oxygen Bomb Calorimeter to Determine Gross Energy and Its Effects on Energy Digestibility of Samples[J]. Chinese Journal of Animal Nutrition, 2021 , 33(2) : 1128 -1136 . DOI: 10.3969/j.issn.1006-267x.2021.02.053

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