Effects of Grain Dietary Fiber on Lipid Metabolism and Intestinal Microflora in Mice with High Cholesterol Diet

FU Nanyan; WU Zhengping; ZHOU Linhua; HAN Xiaoqun; YANG Jing; ZHOU Zhixing

doi:10.3969/j.issn.1006-267x.2021.02.054

Chinese Journal of Animal Nutrition >

2021 , Vol. 33 >Issue 2: 1137 - 1144

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.02.054

Effects of Grain Dietary Fiber on Lipid Metabolism and Intestinal Microflora in Mice with High Cholesterol Diet

FU Nanyan ,
WU Zhengping ,
ZHOU Linhua ,
HAN Xiaoqun ,
YANG Jing ,
ZHOU Zhixing

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Medical College of Yichun University, Yichun 336000, China

Received date: 2020-07-15

Online published: 2021-02-04

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Abstract

The purpose of this study was to investigate the effects of grain dietary fiber (DF) on lipid metabolism and intestinal flora in mice with high cholesterol diet. Thirty C57BL/6J mice were selected and randomly divided into four groups according to body weight. They were fed a basal diet (CO group, n=8) and the basal diet+5 g/kg DF (CO+DF group, n=8), high fat and high cholesterol diet (HF group, n=7) and the high fat and high cholesterol diet+5 g/kg DF diet (HF+DF group, n=7). The experiment period was 8 weeks, and the growth performance, serum and liver biochemical indicators, the contents of fecal total cholesterol (TC), short-chain fatty acid (SCFA), related liver cholesterol and bile acid (BA) protein expression and intestinal flora of each group were measured. The results showed as follows: 1) the final body weight in the CO+DF group was significantly lower than that in the CO group (P<0.05). Compared with the HF group, the CO group and the HF+DF group had significant reductions in body weight at 4 weeks and final (P<0.05), and the weights of liver and fat were significantly reduced (P<0.05). Compared with the HF group, the feeding efficiency in DF+HF group significantly reduced (P<0.05). 2) The contents of TC, triglyceride (TG), the activities of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in serum and the contents of TC and TG in liver in CO+DF group were significantly lower than those in CO group (P<0.05), CO group and HF+DF group were significantly lower than HF group (P<0.05), fecal TC content in HF+DF group was significantly lower than that in the HF group (P<0.05). 3) DF did not affect the protein expression of sterol regulatory element binding protein-2 (SREBP-2), 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR), liver X receptor α (LXRα), microtubule-associated protein 1 light chain 3 (LC3-Ⅰ) and -Ⅱ (P>0.05). The expression level of cholesterol 7α-hydroxylase (Cyp7a1) in the CO+DF group was significantly lower than that in the CO group (P<0.05), but there was no significant difference between the HF and HF+DF groups (P>0.05). 4) The fecal total SCFA and propionate contents in the HF+DF group were significantly higher than those in the HF group (P<0.05). Compared with the corresponding control group, the fecal butyrate content in the CO+DF and HF+DF groups increased significantly (P<0.05). 5) At the phylum level, DF reduced Firmicutes relative abundance, and the relative abundance of Bacteroidetes in the HF+DF group was significantly higher than that in the HF group. The relative abundance of Clostridium ⅪⅤa was significantly increased in the CO+DF and HF+DF groups (P<0.05). In addition, compared to other groups, only the relative abundance of Akkermansia in the HF+DF group increased significantly (P<0.05). In conclusion, it's shown that adding 5 g DF per kilogram of diet can reduce body weight and fat content of mice fed 45% fat diet, reduce the contents of TC and TG and the activities of AST and ALT in serum, the contents of TC and TG in liver, reduce the expression level of cholesterol synthesis-related protein Cyp7a1, and increase total SCFA content, intestinal beneficial flora and the relative abundance of dominant flora.

Key words： dietary fiber; mice; intestinal flora; cholesterol

Cite this article

FU Nanyan , WU Zhengping , ZHOU Linhua , HAN Xiaoqun , YANG Jing , ZHOU Zhixing . Effects of Grain Dietary Fiber on Lipid Metabolism and Intestinal Microflora in Mice with High Cholesterol Diet[J]. Chinese Journal of Animal Nutrition, 2021 , 33(2) : 1137 -1144 . DOI: 10.3969/j.issn.1006-267x.2021.02.054

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