Effects of Resveratrol on Ileal Mucosal Morphology, Antioxidant Capacity, Tight Junction Protein and Inflammatory Factor mRNA Expression in Oxidative Stress Piglets

FU Qingyao; SHI Liguang; ZHOU Hanlin; CAO Ting; MA Zhonghua; JIANG Hongzheng; XUN Wenjuan

doi:10.3969/j.issn.1006-267x.2021.02.057

2021 , Vol. 33 >Issue 2: 1163 - 1172

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.02.057

Effects of Resveratrol on Ileal Mucosal Morphology, Antioxidant Capacity, Tight Junction Protein and Inflammatory Factor mRNA Expression in Oxidative Stress Piglets

FU Qingyao ,
SHI Liguang ,
ZHOU Hanlin ,
CAO Ting ,
MA Zhonghua ,
JIANG Hongzheng ,
XUN Wenjuan

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1. College of Animal Science and Technology, Hainan University, Haikou 570228, China;
2. Institute of Tropical Crop Variety Resources, Chinese Academy of Tropical Agricultural Sciences, Danzhou 571737, China

Received date: 2020-07-06

Online published: 2021-02-04

Supported by

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Abstract

The purpose of this experiment was to study resveratrol (Res) on the intestinal mucosal structure, antioxidant capacity, tight junction protein and inflammatory factor mRNA expression in oxidative stress piglets. Thirty 28-day-old three-way cross (Duroc×Landrace×Yorkshire) weaned piglets with good health and similar parity were selected and randomly divided into 5 groups, with 6 replicates in each group and 1 piglet in each replicate.The control group was fed a basal diet, and the experimental groups were intraperitoneally injected with Diquat+Res of 0, 10, 30 and 90 mg/kg were added to the basal diet, respectively. In the early morning of the 15th day of the experiment, the piglets in the experimental groups (Diquat group, Diquat+10 mg/kg Res group, Diquat+30 mg/kg Res group and Diquat+90 mg/kg Res group) were intraperitoneally injected with Diquat at a dose of 10 mg/kg BW to establish an oxidative stress model, and the control group only injected the same dose of sterilized saline as a reference. The trial period was 21 days. The results showed that: compared with the control group, the villus height and the ratio of villus height/crypt depth (V/C) of the Diquat group were significantly reduced (P<0.05), and the crypt depth was significantly increased (P<0.05); the content of malondialdehyde (MDA) increased significantly (P<0.05), the activity of glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) were significantly reduced in intestinal mucosa (P<0.05), intestinal mucosal interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) contents were significantly increased (P<0.05), while the intestinal mucosal interleukin-10 (IL-10) content was significantly decreased (P<0.05); the relative expression levels of zonules atresia protein-1 (ZO-1), Occludin and transmembrane protein (Claudin) in the intestinal mucosa were significantly decreased (P<0.05). Compared with the Diquat group, the ileal villi height and V/C in the Diquat+30 mg/kg Res group and Diquat+90 mg/kg Res group were significantly increased (P<0.05); the MDA content of the intestinal mucosa was significantly decreased (P<0.05), GSH-Px and SOD activities were significantly increased (P<0.05), among which the Diquat+90 mg/kg Res group had the most significant effect; intestinal mucosal ZO-1, Occludin and Claudin mRNA relative expression levels were all significantly increased (P<0.05), and there was no significant difference among different Res dose groups (P>0.05); intestinal mucosal IL-1β, TNF-α and IL-6 contents were significantly reduced (P<0.05), while IL-10 content was significantly increased (P<0.05). In summary, Res can increase the activity of antioxidant enzymes in the ileum of oxidative stress piglets, improve the morphological structure of the ileal mucosa, inhibit the occurrence of intestinal inflammation and maintain intestinal health.

Key words： resveratrol; piglets; ileal mucosa; oxidative stress; intestinal health

Cite this article

FU Qingyao , SHI Liguang , ZHOU Hanlin , CAO Ting , MA Zhonghua , JIANG Hongzheng , XUN Wenjuan . Effects of Resveratrol on Ileal Mucosal Morphology, Antioxidant Capacity, Tight Junction Protein and Inflammatory Factor mRNA Expression in Oxidative Stress Piglets[J]. Chinese Journal of Animal Nutrition, 2021 , 33(2) : 1163 -1172 . DOI: 10.3969/j.issn.1006-267x.2021.02.057

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