Interaction between Rumen Microbial Density and Short Chain Fatty Acid Concentration in Tibetan Sheep during Warm and Cold Seasons

  • SHI Hao ,
  • SHA Yuzhu ,
  • WEI Hong ,
  • LYU Weibing ,
  • WEN Yuliang ,
  • LIU Xiu ,
  • LUO Yuzhu ,
  • WANG Jiqing ,
  • LI Shaobin ,
  • HU Jiang
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  • Gansu Key Laboratory of Herbivore Biotechnology, College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China

Received date: 2020-07-21

  Online published: 2021-02-04

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Abstract

The adaptation mechanism of Tibetan sheep to the severe nutrient deficiency in the cold season was analyzed in view of the production reality of the serious imbalance of nutrient supply in the warm season and the cold season. In this study, twelve one full year of life (plus or minus 1 months) health of grazing sheep ewes were selected, using gas chromatograph and fluorescence quantitative PCR, respectively detected the rumen short chain fatty acid (SCFA) concentration and microbial flora density of Tibetan sheep in warm (July) and cold (December) season, and analyzed the interaction relationship between rumen SCFA concentration and microbial flora density. The results showed that the concentration and ratio of acetic acid and propionic acid in the rumen of Tibetan sheep in the cold season were significantly higher than those in the warm season (P<0.05), while the other SCFA concentrations were not significantly different between the warm season and the cold season (P>0.05). By analyzing the proportion of various SCFA, it was found that the proportion of acetic acid was significantly higher in the cold season than in the warm season (P<0.05), the proportion of butyric acid was significantly higher in the warm season than in the cold season (P<0.05), and the proportion of isobutyric acid was not significantly different between the warm season and the cold season (P>0.05). As the season change, the relative density of Ruminococcus flavefaciens, Ruminococcus albus, Fibrobacter succinogenes, Butyrivibrio fibrisolvens, Ruminobacter amylophilus and Methanogenic bacteria were significantly higher in the warm season than the cold season (P<0.05). Correlation analysis showed that the concentration of acetic acid and the relative density of Ruminococcus flavefaciens, the concentration of propionic acid and the relative density of Ruminococcus albus had a significant positive correlation (P<0.01), the concentration of acetic acid and the relative density of Ruminococcus albus, Ruminobacter amylophilus, Butyrivibrio fibrisolvens and methanogenic bacteria had a significant negative correlation (P<0.05), butyric acid concentration and 6 kinds of microorganism strains relative density had no significant correlation (P>0.05). Therefore, the interaction between the rumen microflora density of Tibetan sheep and the concentration of SCFA is significantly different, and this interaction mechanism may play an important role in the adaptation of Tibetan sheep to warm and cold seasons. The research results will provide references for the development of Tibetan sheep industry and the protection and utilization of germplasm resources.

Cite this article

SHI Hao , SHA Yuzhu , WEI Hong , LYU Weibing , WEN Yuliang , LIU Xiu , LUO Yuzhu , WANG Jiqing , LI Shaobin , HU Jiang . Interaction between Rumen Microbial Density and Short Chain Fatty Acid Concentration in Tibetan Sheep during Warm and Cold Seasons[J]. Chinese Journal of Animal Nutrition, 2021 , 33(2) : 1191 -1200 . DOI: 10.3969/j.issn.1006-267x.2021.02.060

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