Effects of Feeding Colostrum on Intestinal Permeability and Mucosa-Attached Microbial Composition of Neonatal Goats

YANG Chao; TIAN Quanhua; YANG Li; CHENG Yan; HE Zhixiong; TAN Zhiliang

doi:10.3969/j.issn.1006-267x.2021.03.032

Chinese Journal of Animal Nutrition >

2021 , Vol. 33 >Issue 3: 1503 - 1512

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.03.032

Effects of Feeding Colostrum on Intestinal Permeability and Mucosa-Attached Microbial Composition of Neonatal Goats

YANG Chao ,
TIAN Quanhua ,
YANG Li ,
CHENG Yan ,
HE Zhixiong ,
TAN Zhiliang

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1. Hunan Provincial Key Laboratory of Nutritional Physiology and Metabolic Process, South Central Experimental Station of Animal Nutrition and Feed Science in the Ministry of Agriculture, Hunan Research Center of Livestock & Poultry Sciences, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Key Laboratory for Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China;
2. University of Chinese Academy of Sciences, Beijing 100049, China;
3. School of Environmental Ecology and Biological Engineering, Wuhan Institute of Technology, Wuhan 430205, China;
4. College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China

Received date: 2020-08-24

Online published: 2021-03-18

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Abstract

The objective of this study was to investigate the effects of feeding colostrum on intestinal permeability and mucosa-attached microbial composition of neonatal goats. Sixteen healthy neonatal Ganxi black goats, which had similar birth weight [(1.99±0.08) kg] and did not receive colostrum from their dams, were selected and randomly divided into 2 groups with 8 goats of each group. Goats in milk replacer group and colostrum group received 5% of body weight of milk replacer and bovine colostrum containing 15.43 mg/mL of immunoglobulin G during the first 2 h after birth, respectively. To investigate the changes of intestinal permeability in neonatal goats between 2 groups, six neonatal goats were selected, and 0.5 g mannitol and 1.0 g lactulose were added into milk replacer or colostrum to feed goats. All experimental goats were humanly slaughtered at 12 h after birth; meanwhile, jejunum tissue was collected to determine intestinal mucosa-attached microbial diversity. The results showed as follows: based on 16S sequencing result, the Shannon index of jejunal mucosal-attached microbiota in colostrum group was significantly higher than that in milk replacer group (P<0.05), however, the Simpson index in colostrum group was significantly lower than that in milk replacer group (P<0.05). On the phylum level, the relative abundance of Proteobacteria in jejunal mucosal-attached microbiota in colostrum group was significantly lower than that in milk replacer group (P<0.05), whereas, the relative abundances of Firmicutes and Epsilonbacteraeota were significantly higher than those in milk replacer group (P<0.05). On the genus level, the relative abundance of Escherichia-Shigella in jejunal mucosal-attached microbiota in colostrum group was significantly lower than that in milk replacer group (P<0.05), however, the relative abundances of Lactobacillus and Bifidobacterium were significantly higher than those in milk replacer group (P<0.05). In conclusion, feeding colostrum has no effect on intestinal permeability, but can improve the diversity of intestinal microbiota, promotes the colonization of beneficial bacteria such as Firmicutes, Lactobacillus and Bifidobacterium, inhibits the colonization of harmful bacteria such as Proteobacteria and Escherichia-Shigella in neonatal goats, which is conducive to the establishment of healthy intestinal microbial community in neonatal goats.

Key words： colostrum; neonatal goats; intestinal permeability; mucosa-attached microbial composition

Cite this article

YANG Chao , TIAN Quanhua , YANG Li , CHENG Yan , HE Zhixiong , TAN Zhiliang . Effects of Feeding Colostrum on Intestinal Permeability and Mucosa-Attached Microbial Composition of Neonatal Goats[J]. Chinese Journal of Animal Nutrition, 2021 , 33(3) : 1503 -1512 . DOI: 10.3969/j.issn.1006-267x.2021.03.032

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