Daily Dynamic Changes of Rumen pH, Digestive Enzyme Activities and Protozoa Number in Dairy Cows

  • YANG Baoyu ,
  • WANG Jiao ,
  • YAN Yinan ,
  • AYGUL·Amar ,
  • ZHANG Sujiang
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  • 1. College of Animal Science, Tarim University, Alar 843300, China;
    2. Key Laboratory of Tarim Animal Husbandry Science and Technology, Xinjiang Production & Construction Corps, Alar 843300, China

Received date: 2020-08-31

  Online published: 2021-03-18

Supported by

 

Abstract

The purpose of this experiment was to study the daily dynamic changes of rumen pH, digestive enzyme activities, protozoa number and protozoa species. Three Holstein cows with body weight of (500±30) kg, lactation parity of 2 to 4, good health and permanent rumen fistula were selected as rumen fluid donors. The pre-test period was 14 days and the trial period was 7 days. The results showed as follow: 1) the rumen pH changed regularly with time, that is, the rumen pH was higher before feeding, and the rumen pH decreased rapidly with the diet entering into the rumen after feeding, and then the rumen pH gradually returned to the before feeding level with the decrease of rumen diet, and the rumen pH before and after feeding was significantly different (P<0.05). 2) The activities of protease, amylase and xylanase increased rapidly, while those of pectinase and cellulase increased slowly. Finally, the activities of these five enzymes gradually recovered to the original level, and the activities of the last three enzymes could be maintained at a high level for a long time. The activities of five enzymes were significantly different (P<0.05), except amylase and xylanase activities before and after the second feeding (P>0.05). 3) The results showed that the number of protozoa was higher before feeding, and the content of rumen was diluted after feeding, which led to the decrease of the number of protozoa, and then gradually recovered to or even higher than the original level with the emptying of chyme, but there was no significant difference in the number of protozoa before and after feeding (P>0.05). 4) Morphological identification of the protozoa was carried out, 6 genera and 12 species of protozoa were observed, the genus Dasytricha, Entodinium, Elytroplastron, Eremplastron, Epidinium and Metadinium, of which the most species of the genus Entodinium. The results of this study provide basic data for rumen function regulation and protozoa identification for rumen digestive enzyme activities, microbial flora and protozoa species before and after feeding.

Cite this article

YANG Baoyu , WANG Jiao , YAN Yinan , AYGUL·Amar , ZHANG Sujiang . Daily Dynamic Changes of Rumen pH, Digestive Enzyme Activities and Protozoa Number in Dairy Cows[J]. Chinese Journal of Animal Nutrition, 2021 , 33(3) : 1534 -1544 . DOI: 10.3969/j.issn.1006-267x.2021.03.035

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