Effects of Nutritional Regulation on Reproductive Hormone Contents, Immune Function and Antioxidant Ability of Grazing Pregnant Cattle during Chronic Heat Stress Period

WU Sitong; Aorigrle; WANG Chunjie; Simujide; CHEN Hao; ZHANG Chen; CAO Jiaming; XU Ping; LIU Feihong

doi:10.3969/j.issn.1006-267x.2021.03.036

2021 , Vol. 33 >Issue 3: 1545 - 1554

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.03.036

Effects of Nutritional Regulation on Reproductive Hormone Contents, Immune Function and Antioxidant Ability of Grazing Pregnant Cattle during Chronic Heat Stress Period

WU Sitong ,
Aorigrle ,
WANG Chunjie ,
Simujide ,
CHEN Hao ,
ZHANG Chen ,
CAO Jiaming ,
XU Ping ,
LIU Feihong

Expand

1. College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China;
2. College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, China

Received date: 2020-08-01

Online published: 2021-03-18

Supported by

Fold

Abstract

The purpose of this experiment was to explore the effects of nutritional regulation on the reproductive hormone contents, immune function and antioxidant capacity of grazing pregnant cattle under chronic heat stress. The experiment selected 18 pregnant cows grazing on natural grassland with continuous high temperature in summer, and randomly divided them into a test group (nutrition regulation) and a control group (only grazing), each with 9 pregnant cows. The control group grazed naturally every day. On this basis, after grazing, the test group was fed 2.5 kg of concentrate every day. The pre-trial period was 10 days and the trial period was 60 days. The daily temperature and humidity index of the grassland were recorded. On days 1, 30 and 60 of the test period, blood was collected from the fasted jugular vein in the morning to determine the contents of reproductive hormones and related indicators of immunity and antioxidants, and to compare the difference in serum indexes between test group and control group and the difference between groups (different time points) within test group and control group. The results showed that the experimental animals were in a heat stress environment with a large daily temperature difference for a long time, and the serum progesterone (PROG) and estradiol (E2) contents in the test group were significantly higher than those in the control group on day 60 of test (P<0.05). Serum prolactin (PRL) and triiodothyronine (T3) contents in the test group were significantly increased (P<0.05). During the experiment period, there was no significant difference in serum follicle-stimulating hormone (FSH) content between groups within test group (P>0.05). On day 60 of test, serum interleukin-2 (IL-2) and interleukin-4 (IL-4) contents in the test group were significantly higher than those in the control group (P<0.05), and serum immunoglobulin A (IgA), immunoglobulin M (IgM) and immunoglobulin G (IgG) contents in the test group were increased significantly between groups within test group (P<0.05). There was no significant difference in serum lysozyme (LZ) content between groups (P>0.05). The serum glutathion peroxidase (GSH-Px) activity and total antioxidant capacity (T-AOC) in the test group were significantly increased (P<0.05), and the GSH-Px activity in the test group was significantly higher than that in the control group (P<0.05). There was no significant difference in serum superoxide dismutase (SOD) activity and MDA content between groups within groups (P>0.05), but the SOD activity in the test group tended to be higher than that in the control group. It can be seen that nutritional supplementation effectively improves the immune function and antioxidant capacity of pregnant cows under grazing conditions, and alleviates the hazards of heat stress to the health of beef cattle, and significantly increases the serum reproductive hormone contents of pregnant cattle, which is beneficial to the reproduction performance of cows.

Key words： pregnant cattle; heat stress; reproductive hormones; immune function; antioxidant capacity; nutrition regulation

Cite this article

WU Sitong , Aorigrle , WANG Chunjie , Simujide , CHEN Hao , ZHANG Chen , CAO Jiaming , XU Ping , LIU Feihong . Effects of Nutritional Regulation on Reproductive Hormone Contents, Immune Function and Antioxidant Ability of Grazing Pregnant Cattle during Chronic Heat Stress Period[J]. Chinese Journal of Animal Nutrition, 2021 , 33(3) : 1545 -1554 . DOI: 10.3969/j.issn.1006-267x.2021.03.036

References

[1] 李文杰,连帅,王立鹏.代谢组学在动物应激研究中的应用[J].中国生物制品学杂志,2019,32(4):482-484,488. LI W J,LIAN S,WANG L P,et al.Application of metabolomics in research on animal stress[J].Chinese Journal of Biologicals,2019,32(4):482-484,488.(in Chinese)
[2] DAS R,SAILO L,VERMA N,et al.Impact of heat stress on health and performance of dairy animals:a review[J].Veterinary World,2016,9(3):260-268.

[3] ALLAKHVERDIEV S I,KRESLAVSKI V D,KLIMOV V V,et al.Heat stress:an overview of molecular responses in photosynthesis[J].Photosynthesis Research,2008,98(1/2/3):541-550.
[4] DE VRIES A,RISCO C A.Trends and seasonality of reproductive performance in Florida and Georgia dairy herds from 1976 to 2002[J].Journal of Dairy Science,2005,88(9):3155-3165.

[5] SARTORI R,SARTOR-BERGFELT R,MERTENS S A,et al.Fertilization and early embryonic development in heifers and lactating cows in summer and lactating and dry cows in winter[J].Journal of Dairy Science,2002,85(11):2803-2812.

[6] 赵娟娟,冯曼,孙新胜,等.河北省舍饲绵羊的热应激评价及温湿参数与羊生理指标的相关性研究[J].畜牧兽医学报,2020,51(6):1342-1353. ZHAO J J,FENG M,SUN X S,et al.Evaluation of heat stress of housed sheep and correlation between temperature-humidity parameter and physiological parameter of sheep in Hebei province[J].Chinese Journal of Animal and Veterinary Sciences,2020,51(6):1342-1353.(in Chinese)
[7] 孔庆娟,郭丹丹,王铁岗.热应激对奶牛生长、繁殖性能及健康的研究进展[J].中国饲料,2020(8):5-8. KONG Q J,GUO D D,WANG T G,et al.Research progress of heat stress on growth,reproductive performance and health of dairy cows[J].China Feed,2020(8):5-8. (in Chinese)
[8] SILANIKOVE N,KOLUMAN N.Impact of climate change on the dairy industry in temperate zones:predications on the overall negative impact and on the positive role of dairy goats in adaptation to earth warming[J].Small Ruminant Research,2015,123(1):27-34.

[9] KADOKAWA H,SAKATANI M,HANSEN P J.Perspectives on improvement of reproduction in cattle during heat stress in a future Japan[J].Animal Science Journal,2012,83(6):439-445.

[10] 彭孝坤,胡建宏,张恩平.热应激对肉牛和肉羊生理生化指标及外周血miRNA表达水平的影响[J].家畜生态学报,2018,39(3):1-7. PENG X K,HU J H,ZHANG E P,et al.Effects of heat stress on physiological and biochemical indexes and expression levels of mirna in peripheral blood of beef cattle,sheep and goats[J].Journal of Domestic Animal Ecology,2018,39(3):1-7. (in Chinese)
[11] MIN L,LI D G,TONG X,et al.Nutritional strategies for alleviating the detrimental effects of heat stress in dairy cows:a review[J].International Journal of Biometeorology,2019,63(9):1283-1302.

[12] RHOADS R P,BAUMGARD L H,SUAGEE J K,et al.Nutritional interventions to alleviate the negative consequences of heat stress[J].Advances in Nutrition,2013,4(3):267-276.

[13] 扎木嘎.不同营养水平日粮对母羊繁殖性能影响的研究[D].硕士学位论文.呼和浩特:内蒙古农业大学,2016:17-21. ZHA M G.The study of effects of different nutrition levels diet on the reproductive performance of ewes[D].Master's Thesis.Hohhot:Inner Mongolia Agricultural University,2016:17-21. (in Chinese)
[14] 闫方权.热应激奶牛日粮适宜净能需要量的研究[D].硕士学位论文.雅安:四川农业大学,2015:15-16. YAN F Q.Study on dietary optimum net energy level in dairy cows under heat stress[D].Master's Thesis.Ya'an:Sichuan Agricultural University,2015:15-16. (in Chinese)
[15] 陈浩,王纯洁,斯木吉德,等.育成期补饲对放牧牛生长性能、体尺指标及血清抗氧化、生化指标的影响[J].动物营养学报,2020,32(11):5267-5274. CHEN H,WANG C J,SI M J D,et al.Effects of supplementary feeding on growth performance,body size indexes and serum antioxidant,biochemical indexes of grazing cows during growing period[J].Chinese Journal of Animal Nutrition,2020,32(11):5267-5274.(in Chinese)
[16] ROSS J W,HALE B J,SEIBERT J T,et al.Molecular mechanisms through which heat stress compromises reproduction in pigs[J].Journal of Animal Science,2019,97(Suppl.2):120.
[17] 罗玉江.北疆部分规模化牛场奶牛BVD,IBR,BPI和Brucellosis的流行病学调查[D].硕士学位论文.石河子:石河子大学,2015:34-48. LUO Y J.Epidemiological investigation of BVD,IBR,BPI and brucellosis in part of scale dairy farms of the Northern Xinjiang[D].Master's Thesis.Shihezi:Shihezi University,2015:34-48. (in Chinese)
[18] 唐攀喜.叶黄素-中草药复方合剂对高温环境中蛋鸡生产性能和蛋品质的影响[D].硕士学位论文.湛江:广东海洋大学,2013:67-82. TANG P X.Influence of lutein-Chinese herbal mixture on performance and egg quality of Lohmann laying hen during high environmental temperature[D].Master's Thesis.Zhanjiang:Guangdong Ocean University,2013:67-82.(in Chinese)
[19] 宋代军,何钦,姚焰础.热应激对不同泌乳阶段奶牛生产性能和血清激素浓度的影响[J].动物营养学报,2013,25(10):2294-2302. SONG D J,HE Q,YAO Y C.Effects of heat stress on performance and serum hormone concentrations of dairy cows at different lactation stages[J].Chinese Journal of Animal Nutrition,2013,25(10):2294-2302. (in Chinese)
[20] 李朝明.荷斯坦奶牛、娟姗牛和娟荷杂交牛热适应性的研究[D].硕士学位论文.重庆:西南大学,2013:45-62. LI C M.Study on heat tolerance of Jersey,Holstein and Jersey X Holstein dairy cows[D].Master's Thesis.Chongqing:Southwest University,2013:45-62. (in Chinese)
[21] MANDEL S J,SPENCER C A,HOLLOWELL J G.Are detection and treatment of thyroid insufficiency in pregnancy feasible?[J].Thyroid,2005,15(1):44-53.

[22] 李妍,李晓蒙,李秋凤,等.不同营养水平日粮对奶公牛直线育肥性能的影响[J].草业学报,2016,25(1):273-279. LI Y,LI X M,LI Q F,et al.Nutrition effects on growth of Holstein bulls[J].Acta Prataculturae Sinica,2016,25(1):273-279. (in Chinese)
[23] 宋宏立,王清,林英庭.日粮中果寡糖对奶山羊血清生化指标、抗氧化指标及免疫机能的影响[J].畜牧与兽医,2019,51(8):41-46. SONG H L,WANG Q,LIN Y T.Effects of fructooligosaccharide in diet on serum bichemical indices,antioxidant indices and immune function of dairy goat[J].Animal Husbandry & Veterinary Medicine,2019,51(8):41-46. (in Chinese)
[24] 烟玉华,韩秀欣.畜禽生产影响因素之温差[J].养殖与饲料,2014(7):20-21. YAN Y H,HAN X X.Temperature difference of factors affecting livestock production[J].Animals Breeding and Feed,2014(7):20-21. (in Chinese)
[25] 刘嘉莉,窦金焕,胡丽蓉,等.热应激对奶牛生理和免疫功能的影响及其机理[J].中国畜牧兽医,2018,45(1):263-270. LIU J L,DOU J H,HU L R,et al.Effects and mechanism of heat stress on physiological and immune system in dairy cows[J].China Animal Husbandry & Veterinary Medicine,2018,45(1):263-270. (in Chinese)
[26] 石璐璐,王哲奇,徐元庆,等.热应激对绵羊血清免疫和抗氧化指标及相关基因相对表达量的影响[J].动物营养学报,2020,32(11):5275-5284. SHI L L,WANG Z Q,XU Y Q,et al.Effects of heat stress on serum immune and antioxidative indexes and relative expression of related genes in sheep[J].Chinese Journal of Animal Nutrition,2020,32(11):5275-5284.(in Chinese)
[27] JAUNIAUX E,WATSON A L,HEMPSTOCK J,et al.Onset of maternal arterial blood flow and placental oxidative stress:a possible factor in human early pregnancy failure[J].The American Journal of Pathology,2000,157(6):2111-2122.

[28] MYATT L,CUI X L.Oxidative stress in the placenta[J].Histochemistry and Cell Biology,2004,122(4):369-382.

[29] 王玲,罗宗刚,蔡明成,等.热应激对肉牛血清离子、酶活性、抗氧化及免疫指标的影响[J].西南师范大学学报(自然科学版),2017,42(12):37-41. WANG L,LUO Z G,CAI M C,et al.Effect of heat stress on serum ionic concentration,enzymatic activity,antioxidant and immune index in beef cattle[J].Journal of Southwest China Normal University (Natural Science Edition),2017,42(12):37-41. (in Chinese)

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article

References