Optimization of Enzymatic Hydrolysis Conditions of Rapeseed Meal by Response Surface Method

ZHU Xiaofeng; ZHANG Zhen; DING Liren; HANG Suqin

doi:10.3969/j.issn.1006-267x.2021.03.051

Chinese Journal of Animal Nutrition >

2021 , Vol. 33 >Issue 3: 1708 - 1715

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.03.051

Optimization of Enzymatic Hydrolysis Conditions of Rapeseed Meal by Response Surface Method

ZHU Xiaofeng ,
ZHANG Zhen ,
DING Liren ,
HANG Suqin

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1. National Joint Research Center for Animal Digestive Tract Nutrition, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China;
2. National Experimental Teaching Center for Animal Science, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China

Received date: 2020-08-14

Online published: 2021-03-18

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Abstract

Rapeseed meal (RSM) is a potential source for animal feed due to its relatively high crude protein content (32% to 40%). However, the high fiber content in RSM has limited its application in pig and poultry diets. The aim of this study was to optimized the conditions for enzymatic hydrolysis of RSM by complex fiber-degrading enzyme (cellulase+pectinase) through the response surface method to obtain a higher yield of reducing sugar and lower fiber content. Method: 1) the enzymatic hydrolysis conditions of pH (3.2 to 4.8), temperature (45 to 55 ℃), enzymatic time (18 to 24 h) and substrate concentration (RSM concentration, 100 to 200 g/L) were optimized by response surface experiment; 2) RSM was enzymatic hydrolyzed under the optimal conditions (optimum group), and the enzymatic hydrolysis conditions before optimization were used as a control (control group) to investigate the optimization effect. Results: 1) response surface experiment results showed that pH, temperature and enzymatic time had extremely significant effects on reducing sugar conversion ratio (P<0.01). The pH×temperature and temperature×enzymatic time had significant interactions on reducing sugar conversion ratio (P<0.05). 2) Compared with the control group (4.8 for pH, 50 ℃ for temperature, 24 h for enzymatic time, and 100 g/L for substrate concentration), the reducing sugar conversion ratio in the optimum group (4.0 for pH, 50 ℃ for temperature, 28 h for enzymatic time, and 200 g/L for substrate concentration) was significantly increased (P<0.05), and the contents of neutral detergent fiber and acid detergent fiber were significantly decreased (P<0.05). Conclusion: 1) the enzymatic hydrolysis conditions optimized by response surface method can effectively increase the reducing sugar production and reduce the fiber content of RSM; 2) the optimized conditions are 4.0 for pH, 50 ℃ for temperature, 28 h for enzymatic time, and 200 g/L for substrate concentration.

Key words： response surface method; cellulase; pectinase; enzymatic hydrolysis conditions; rapeseed meal; reducing sugar; fiber content

Cite this article

ZHU Xiaofeng , ZHANG Zhen , DING Liren , HANG Suqin . Optimization of Enzymatic Hydrolysis Conditions of Rapeseed Meal by Response Surface Method[J]. Chinese Journal of Animal Nutrition, 2021 , 33(3) : 1708 -1715 . DOI: 10.3969/j.issn.1006-267x.2021.03.051

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