Effects of Tannin on Rumen Protozoa Number and Population Diversity in Sheep

  • NIU Xiaoyu ,
  • LI Dabiao ,
  • LI Li ,
  • XIE Yongfang ,
  • ZHU Shouqian ,
  • WANG Jingyao
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  • Inner Mongolia Key Laboratory of Animal Nutrition and Feed Science, College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China

Received date: 2020-08-05

  Online published: 2021-03-18

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Abstract

The purpose of this experiment was to investigate the effects of different levels of tannin in the diet on the number and population diversity of protozoa in the rumen of sheep.A total of 15 healthy sheep with weight about 45 kg were selected and randomly divided into 3 groups: group Ⅰ (control group, without tannin), group Ⅱ (2% tannin) and group Ⅲ (4% tannin),with 5 sheep in each group. The pre-trial period was 14 days and the formal trial period was 20 days. The rumen fluid was collected at days 18 and 19 during the trial period before morning feeding and after morning feeding for 6 h using rumen fluid oral sampler. Microscope counting method was used to detect the number of protozoa in sheep rumen fluid samples. High-throughput sequencing technology was used to detect the population diversity. The results showed as follows: 1) before morning feeding and after morning feeding for 6 h, the number of protozoa in the group Ⅲ was significantly lower than that in the control group (P<0.05), and the group Ⅱ had a tendency to be lower than the control group, but the statistical analysis was not significant (P>0.05). 2) Diets containing 2% and 4% tannins significantly reduced the Chao 1 index of sheep rumen protozoa (P<0.05), Shannon index, Simpson index and Ace index had a decreasing trend (P>0.05). 3) At the genus level, the relative abundance of Entodinium of rumen protozoa in group Ⅲ was significantly higher than that in control group and group Ⅱ (P<0.05); the relative abundance of Epidinium of group Ⅱ and group Ⅲ tended to be lower than control group (P=0.06). The relative abundance of Isotricha in group Ⅱ and group Ⅲ tended to be lower than control group (P=0.07); the relative abundance of other protozoa in the rumen had no significant difference compared with control group (P>0.05). It can be seen that when the tannin content in the diet is 2% and 4%, the number of protozoa in the rumen fluid of sheep is significantly reduce; it has no significant effect on the diversity of protozoan population.

Key words: sheep; tannin; protozoa; diversity

Cite this article

NIU Xiaoyu , LI Dabiao , LI Li , XIE Yongfang , ZHU Shouqian , WANG Jingyao . Effects of Tannin on Rumen Protozoa Number and Population Diversity in Sheep[J]. Chinese Journal of Animal Nutrition, 2021 , 33(3) : 1783 -1791 . DOI: 10.3969/j.issn.1006-267x.2021.03.059

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