Effects of Compound Plant Extracts on Performance, Intestinal Mucosal Morphology and Cecal Microflora Structure of Laying Hens

CHEN Xiangyu; GENG Haochuan; HOU Jianku; HOU Fei; LI Fuwei; WANG Fei; ZHU Yahao; LIU Meng; ZHOU Rongyan; CHEN Yifan; WANG Dehe; CHEN Hui

doi:10.3969/j.issn.1006-267x.2021.04.023

2021 , Vol. 33 >Issue 4: 2033 - 2043

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.04.023

Effects of Compound Plant Extracts on Performance, Intestinal Mucosal Morphology and Cecal Microflora Structure of Laying Hens

CHEN Xiangyu ,
GENG Haochuan ,
HOU Jianku ,
HOU Fei ,
LI Fuwei ,
WANG Fei ,
ZHU Yahao ,
LIU Meng ,
ZHOU Rongyan ,
CHEN Yifan ,
WANG Dehe ,
CHEN Hui

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1. College of Animal Science and Technology, Agricultural University of Hebei, Baoding 071001, China;
2. Xinji City Center for Animal Disease Prevention and Control, Xinji 052360, China;
3. Agricultural and Rural Bureau of Shunping County of Baoding City, Baoding 071001, China;
4. Institute of Poultry Science, Shandong Academy of Agricultural Sciences, Ji'nan 250023, China;
5. Beijing Dayou Hanteng Animal Husbandry Technology Co., Ltd., Beijing 101300, China

Received date: 2020-08-30

Online published: 2021-04-15

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Abstract

The purpose of this experiment was conducted to study the effects of adding compound plant extracts with different levels to the diet on the performance, intestinal mucosal morphology and cecal microflora structure of laying hens. A total of 960 healthy Hy-Line gray hens at the age of 42 weeks with similar body weight and laying rate were selected and randomly divided into 4 groups with 5 replicates in each group and 48 chickens in each replicate. Laying hens in control group were fed a basal diet, and those in test groups Ⅰ, Ⅱ and Ⅲ were fed experimental diets which supplemented with 150, 300 and 450 mg/kg of compound plant extracts into the basal diet, respectively. The pre-feeding period was 10 d and the formal period was 56 d. The results showed as follows:1) compared with the control group, diet supplemented with 300 and 450 mg/kg compound plant extracts significantly increased the laying rate of laying hens (P<0.05). 2) Compared with the control group, the crypt depth of jejunum and duodenum was significantly reduced (P<0.05), and the ratio of villi height to crypt depth of duodenum was significantly increased when the diet supplemented with 300 mg/kg compound plant extracts (P<0.05). 3) In the term of cecal microflora structure, at the phylum level, diet supplemented with 300 and 450 mg/kg compound plant extracts significantly increased the relative abundance of Bacteroidetes (P<0.05), and diet supplemented with 450 mg/kg compound plant extracts significantly decreased the relative abundance of Firmicutes compared with the control group (P<0.05); at the genus level, the relative abundance of Bacteroides in the group supplemented with 450 mg/kg compound plant extracts was significantly increased (P<0.05), and the relative abundance of Akkermansia had an increasing trend compared with the control group (P=0.063). In summary, under the condition of this experiment, adding 300 mg/kg compound plant extracts into the diet can increase the laying rate of laying hens, reduce the crypt depth of jejunum and duodenum, increase the ratio of villi height to crypt depth of duodenum, increase the relative abundance of Bacteroidetes in cecal microflora, and then improve the intestinal mucosal morphology and cecal microflora structure, and increase the performance of laying hens.

Key words： compound plant extracts; laying hens; intestinal mucosal morphology; cecal microflora

Cite this article

CHEN Xiangyu , GENG Haochuan , HOU Jianku , HOU Fei , LI Fuwei , WANG Fei , ZHU Yahao , LIU Meng , ZHOU Rongyan , CHEN Yifan , WANG Dehe , CHEN Hui . Effects of Compound Plant Extracts on Performance, Intestinal Mucosal Morphology and Cecal Microflora Structure of Laying Hens[J]. Chinese Journal of Animal Nutrition, 2021 , 33(4) : 2033 -2043 . DOI: 10.3969/j.issn.1006-267x.2021.04.023

References

[1] 金立志,杨江涛.植物提取物的抗氧化特性及其在动物无抗饲料中的应用研究进展[J].中国畜牧杂志,2020,56(4):29-34. JIN L Z,YANG J T.Antioxidative actions of phytogenics and their application in AGP-free diets for domestic animals[J].Chinese Journal of Animal Science,2020,56(4):29-34.(in Chinese)
[2] 肖传明,王蕾,黄远荣,等.常见植物提取物在饲料中的主要应用研究进展[J].广东饲料,2016,25(12):29-31. XIAO C M,WANG L,HUANG Y R,et al.Research progress on main application of common plant extracts in feed[J].Guangdong Feed,2016,25(12):29-31.(in Chinese)
[3] 王晓杰,黄立新,张彩虹,等.植物提取物饲料添加剂的研究进展[J].生物质化学工程,2018,52(3):50-58. WANG X J,HUANG L X,ZHANG C H,et al.Research development of botanical extract as feed additive in poultry[J].Biomass Chemical Engineering,2018,52(3):50-58.(in Chinese)
[4] 徐腾腾,张腾龙,王丽芳,等.复合植物提取物对奶牛生产性能及血清免疫、抗氧化指标的影响[J].动物营养学报,2019,31(12):5707-5718. XU T T,ZHANG T L,WANG L F,et al.Effects of compound plant extracts on production,serum immune and antioxidant indexes of dairy cows[J].Chinese Journal of Animal Nutrition,2019,31(12):5707-5718.(in Chinese)
[5] 陶勇,吉慧帆,吴楚筠,等.复合植物提取物对苏姜猪生长性能、养分消化率及粪中微生物比例的影响[J].西北农业学报,2019,28(12):1942-1947. TAO Y,JI H F,WU C J,et al.Effects of compound plant extracts on growth performance,nutrient apparent digestibility and manure microbial proportion in Sujiang pigs[J].Acta Agriculturae Boreali-Occidentalis Sinica,2019,28(12):1942-1947.(in Chinese)
[6] 秦兴然,王莉,叶宝娜,等.浅谈大蒜素在蛋鸡生产中的应用[J].中国畜牧兽医文摘,2015,31(5):224. QIN X R,WANG L,YE B N,et al.Talking about the application of allicin in layer production[J].Abstracts of Chinese Animal Husbandry and Veterinary,2015,31(5):224.(in Chinese)
[7] KHAN S H,SARDAR R,ASHRAF M A.Effects of dietary garlic on performance and serum and egg yolk cholesterol concentration in laying hens[J].Asian Journal of Poultry Science,2007,1(1):22-27.
[8] 何云,王浓章,周培军,等.大蒜、杜仲添加剂对雏鸡生产性能和肠道微生物的影响[J].兽药与饲料添加剂,2004,9(4):6-9. HE Y,WANG N Z,ZHOU P J,et al.Effects of garlic and eucommia ulmoides additives on performance and intestinal microbes of chicks[J].Veterinary Pharmaceuticals and Feed Additives,2004,9(4):6-9.(in Chinese)
[9] 赵姝娴,贾俊涛,赵宇飞.枯草芽孢杆菌和菊粉对产蛋后期蛋鸡生产性能、蛋品质及肠道绒毛形态的影响[J].中国饲料,2018(24):44-48. ZHAO S X,JIA J T,ZHAO Y F.Dietary supplementation of bacillus subtilis and inulin on laying performance,egg quality and intestinal morphology of laying hens in late production phase[J].China Feed,2018(24):44-48.(in Chinese)
[10] 蔡超,魏艳红,曲湘勇,等.菊粉的生物学功能及其在畜禽生产中的应用[J].中国饲料,2013(3):37-40. CAI C,WEI Y H,QU X Y,et al.Biochemical function of inulin and its application in livestock and poultry production[J].China Feed,2013(3):37-40.(in Chinese)
[11] 徐静,张子儒,王德贺,等.饮水中添加大蒜精油对蛋鸡生长性能、肠道组织形态及盲肠菌群的影响[J].动物营养学报,2021,33(1):308-316. XU J,ZHANG Z R,WANG D H,et al.Effects of adding garlic essential oil into drinking water on growth performance,intestinal tissue morphology and cecum microbial flora of layer hens[J].Chinese Journal of Animal Nutrition,2021,33(1):308-316.(in Chinese)
[12] 奚文博,严昌国,崔莲花.益生元-菊粉在动物营养中的研究进展[J].饲料研究,2017(21):8-12,21. XI W B,YAN C G,CUI L H.Research progress of prebiotic-inulin in animal nutrition[J].Feed Research,2017(21):8-12,21.(in Chinese)
[13] 卢德勋.饲料营养活性物质(nutricines):一个亟待重新审视的研究领域[J].饲料工业,2020,41(3):1-5. LU D X.Feed nutricines:a research area that is worth to examine closely[J].Feed Industry,2020,41(3):1-5.(in Chinese)
[14] 易中华,张建云.膳食纤维与菊粉、低聚果糖对肠道生理功能的影响[J].中国动物保健,2008(12):102-104. YI Z H,ZHANG J Y.The influence of dietary fiber,inulin and oligofructose on the physiological function of intestine[J].China Animal Health,2008(12):102-104.(in Chinese)
[15] 陈泽,上官明军,杜彬,等.日粮中添加不同水平菊粉对白羽肉鸡生产的影响[J].山西农业科学,2017,45(9):1530-1533. CHEN Z,SHANGGUAN M J,DU B,et al.Effects of different levels of inulin in daily ration on performance of white feather broilers[J].Journal of Shanxi Agricultural Sciences,2017,45(9):1530-1533.(in Chinese)
[16] 徐静,陈一凡,王德贺,等.大蒜精油对0-4周龄蛋雏鸡生长性能、肠道组织形态、免疫机能和血清生化指标的影响[J].动物营养学报,2020,32(12):5684-5691. XU J,CHEN Y F,WANG D H,et al.Effects of garlic essential oil on growth performance,intestinal tissue morphology,immune function and serum biochemical indexes of 0 to 4 weeks old layer chickens[J].Chinese Journal of Animal Nutrition2020,32(12):5684-5691.(in Chinese)
[17] 王凡,张学科,张学艳,等.大蒜素对蛋雏鸡免疫器官抗氧化能力及生产性能的影响[J].饲料博览,2019(10):19-23. WANG F,ZHANG X K,ZHANG X Y,et al.Effects of allicin on immune organs antioxidant function and growth performance of laying hens[J].Feed Review,2019(10):19-23.(in Chinese)
[18] 金永才,刘璇,王国伟,等.复合植物提取物对"农大3号"小型蛋鸡父母代种鸡产蛋性能和种蛋品质的影响[J].中国家禽,2020,42(3):103-105. JIN Y C,LIU X,WANG G W,et al.Effects of compound plant extracts on laying performance and egg quality of dwarf parent stock layer of Nongda No.3[J].China Poultry,2020,42(3):103-105.(in Chinese)
[19] WANG M,YANG C,WANG Q Y,et al.The growth performance,intestinal digestive and absorptive capabilities in piglets with different lengths of small intestines[J].Animal,2020,14(6):1196-1203.

[20] 张耀文,马文峰,张志丹,等.天蚕素抗菌肽对产蛋后期蛋鸡生产性能、蛋品质及肠道黏膜形态的影响[J].家畜生态学报,2020,41(5):30-35. ZHANG Y W,MA W F,ZHANG Z D,et al.Effects of antimicrobial peptides on laying performance,egg quality and intestinal morphology of hens during the late laying period[J].Journal of Domestic Animal Ecology,2020,41(5):30-35.(in Chinese)
[21] 周建民,马友彪,张海军,等.白酒糟酵母培养物对肉仔鸡生长性能、血清抗氧化指标和肠道形态结构的影响[J].动物营养学报,2019,31(5):2357-2366. ZHOU J M,MA Y B,ZHANG H J,et al.Effects of yeast culture using distiller's grains on growth performance,serum antioxidant indices and intestinal morphology of broilers[J].Chinese Journal of Animal Nutrition,2019,31(5):2357-2366.(in Chinese)
[22] JIN L Z,BAIDOO S K,MARQUARDT R R,et al.In vitro inhibition of adhesion of enterotoxigenic Escherichia coli K88 to piglet intestinal mucus by egg-yolk antibodies[J].FEMS Immunology & Medical Microbiology,1998,21(4):313-321.

[23] PLAZA-DÍAZ J,RUIZ-OJEDA F J,GIL-CAMPOS M,et al.Immune-mediated mechanisms of action of probiotics and synbiotics in treating pediatric intestinal diseases[J].Nutrients,2018,10(1):42.
[24] 陶振阳,张耀文,曹忠洋,等.粪肠球菌对产蛋后期蛋鸡生产性能、蛋品质及肠道组织形态的影响[J].中国畜牧兽医,2020,47(5):1421-1428. TAO Z Y,ZHANG Y W,CAO Z Y,et al.Effects of Enterococcus faecalis on Production performance,egg quality and intestinal morphology of laying hens during the late laying period[J].Chinese Animal Husbandry and Veterinary Medicine,2020,47(5):1421-1428.(in Chinese)
[25] 赵铭伟,奉玲丽,张琰芳,等.鞣花酸对断奶仔猪小肠黏膜形态及肠黏膜屏障功能的影响[J].黑龙江畜牧兽医,2020(21):126-130,135. ZHAO M W,FENG L L,ZHANG Y F,et al.The effect of ellagic acid on the intestinal mucosal morphology and intestinal barrier function in weaned piglets[J].Heilongjiang Animal Science and Veterinary Medicine,2020(21):126-130,135.(in Chinese)
[26] YOUNG V B.The intestinal microbiota in health and disease[J].Current Opinion in Gastroenterology,2012,28(1):63-69.

[27] 徐凤文.饲料中添加绞股蓝粉对蛋鸡生产性能、蛋品质、血清生化指标和盲肠微生物区系影响的研究[D].硕士学位论文.杨凌:西北农林科技大学,2019. XU F W.Effect of gypenia powder added to feed on performance,egg quality,serum biochemical indexes and caecum microflora of laying hens[D].Master's Thesis.Yangling:Northwest A&F University,2019.(in Chinese)
[28] 肖发沂,孙汝江.植物提取物在家禽生产中的应用进展[J].家禽科学,2018(2):51-53. XIAO F Y,SUN R J.Research progress on plant extracts in poultry production[J]Poultry Science,2018(2):51-53.(in Chinese)
[29] GRICE E A,KONG H H,CONLAN S,et al.Topographical and temporal diversity of the human skin microbiome[J].Science,2009,324(5931):1190-1192.

[30] 曾李,倪冬姣,熊爱军,等.发酵茶籽粕对海兰褐蛋鸡产蛋性能、鸡蛋品质、血清生化指标和肠道菌群结构的影响[J].国外畜牧学(猪与禽),2020,40(3):75-80. ZENG L,NI D J,XIONG A J,et al.Effect of fermented tea seed meal on laying performance,egg quality,serum biochemical indexes and intestinal flora structure of Hailan brown layer[J].Animal Science Abroad (Pigs & Poultry),2020,40(3):75-80.(in Chinese)
[31] 徐帅,林奕岑,周梦佳,等.基于高通量测定肉鸡回肠微生物多样性及PICRUSt基因预测分析[J].动物营养学报,2016,28(8):2581-2588. XU S,LIN Y C,ZHOU M J,et al.Analyzing of ileum microbial diversity of chickens by high-throughput sequencing and PICRUSt predicted[J].Chinese Journal of Animal Nutrition,2016,28(8):2581-2588.(in Chinese)
[32] 张亚楠,魏单平,韩瑞丽,等.高产期不同产蛋水平蛋鸡肠道微生物群落特征[J].中国兽医学报,2017,37(6):1179-1185. ZHANG Y N,WEI S P,HAN R L,et al.The character of the intestinal microbiota associated with laying performance in hens[J].Chinese Journal of Veterinary Science,2017,37(6):1179-1185.(in Chinese)
[33] 张璐,周林康,廖榕玉,等.高脂饮食中添加短链菊粉对小鼠肠道菌群的影响[J].现代生物医学进展,2017,17(22):4201-4206,4258. ZHANG L,ZHOU L K,LIAO R Y,et al.Effect of short-chain inulin supplement on the gut microbiota in mice fed by high fat diet[J].Progress in Modern Biomedicine,2017,17(22):4201-4206,4258.(in Chinese)
[34] 王俊,虞龙飞,张祺满,等.植物提取物和抗生素对肉鸡生产性能和肠道菌群和代谢功能的研究[C]//中国畜牧兽医学会动物微生态学分会第五届第十三次全国学术研讨会论文集.广州:中国畜牧兽医学会,2018:240. WANG J,YU L F,ZHANG Q M,et al.On the effect of extracts and antibiotics on broiler performance,intestinal flora and metabolic function[C]//Proceedings of the Fifth 13th National Academic Symposium of the Animal Microecology Branch of the Chinese Society of Animal Husbandry and Veterinary Medicine.Guangzhou:Chinese Society of Animal Husbandry and Veterinary Medicine,2018:240.(in Chinese)
[35] HOOPER L V.Bacterial contributions to mammalian gut development[J].Trends in Microbiology,2004,12(3):129-134.

[36] 杨建平,王笑笑,李新锋,等.饲粮中添加复合植物精油对58周龄海兰褐壳蛋鸡生产性能、蛋品质和肠道菌群结构的影响[J].动物营养学报,2020,32(6):2869-2879. YANG J P,WANG X X,LI X F,et al.Effects of compound plant essential oils on performance,egg quality and cecal microflora of 58-week-old Hy-Line brown laying hens[J].Chinese Journal of Animal Nutrition,2020,32(6):2869-2879.(in Chinese)
[37] GRANDER C,ADOLPH T E,WIESER V,et al.Recovery of ethanol-induced Akkermansia muciniphila depletion ameliorates alcoholic liver disease[J].Gut,2018,67(5):891-901.

[38] BRAHE L K,LE CHATELIER E,PRIFTI E,et al.Specific gut microbiota features and metabolic markers in postmenopausal women with obesity[J].Nutrition & Diabetes,2015,5(6):e159.
[39] NAGAO-KITAMOTO H,LESLIE J L,KITAMOTO S,et al.Interleukin-22-mediated host glycosylation prevents Clostridioides difficile infection by modulating the metabolic activity of the gut microbiota[J].Nature Medicine,2020,26(4):608-617.

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