Effects of Betaine on Growth Performance, Organ Indices, Serum Biochemical Parameters and Spleen Inflammatory Factor mRNA Expression of Geese Challenged by Lipopolysaccharide

YANG Jinjin; YANG Zhi; YANG Yu; LING Jingru; XIAO Xia; YANG Haiming; WANG Zhiyue

doi:10.3969/j.issn.1006-267x.2021.04.024

2021 , Vol. 33 >Issue 4: 2044 - 2054

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.04.024

Effects of Betaine on Growth Performance, Organ Indices, Serum Biochemical Parameters and Spleen Inflammatory Factor mRNA Expression of Geese Challenged by Lipopolysaccharide

YANG Jinjin ,
YANG Zhi ,
YANG Yu ,
LING Jingru ,
XIAO Xia ,
YANG Haiming ,
WANG Zhiyue

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1. College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China;
2. Institutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou 225009, China

Received date: 2020-08-31

Online published: 2021-04-15

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Abstract

The present study aimed to investigate the effects of dietary betaine (Bet) on growth performance, organ indices, serum biochemical parameters and spleen inflammatory factor expression of geese challenged by lipopolysaccharide (LPS). The experiment was designed as a 2×2 double-factor trial design and the principal factors were LPS (intraperitoneal injection of LPS or physiological saline) and Bet (dietary no added or added 0.06% Bet). A total of 168 healthy fifteen-day-old Jiangnan white male geese with similar body weight were selected and randomly divided into 4 groups:Ctrl group (control group, no added Bet+intraperitoneal injection 1.5 mg/kg BW physiological saline), Bet group (added 0.06% Bet+intraperitoneal injection 1.5 mg/kg BW physiological saline), LPS group (no added Bet+intraperitoneal injection 1.5 mg/kg BW LPS) and Bet+LPS group (added 0.06% Bet+intraperitoneal injection 1.5 mg/kg BW LPS), with 6 replicates per group and 7 geese per replicate. Geese were injected in the morning at 16, 18 and 20 days of age,respectively. At 21 and 28 days of age, geese weighed and slaughtered. The experiment were divided into stress period (16 to 21 days of age) and recovery period (22 to 28 days of age). The results showed as follows:1) during the stress period, LPS challenge significantly decreased the body weigh at 21 days of age, average daily feed intake (ADFI) and average daily gain (ADG) of geese (P≤0.05), and significantly increased the ratio of feed to gain (F/G) (P≤0.05); adding betaine had a tendency to increase the ADFI (P=0.080) and body weigh at 21 days of age of geese (P=0.090); LPS challenge and adding betaine had significant interaction on ADFI of geese (P≤0.05), and had a tendency of significant interaction on the body weigh at 21 days of age (P=0.075). During the recovery period, LPS challenge significantly decreased the body weigh at 28 days of age, ADFI and F/G of geese (P≤0.05), and had no significant effect on ADG (P>0.05); adding betaine had no significant effect on the growth performance of geese (P>0.05). 2) At 21 days of age, LPS challenge significantly decrease the thymus index of geese (P≤0.05), while adding betaine significantly increased the thymus index of geese (P≤0.05). 3) At 21 days of age, LPS challenge significantly decrease the serum albumin content and albumin/globulin (P≤0.05), and significantly increased the serum globulin content of geese(P≤0.05); LPS challenge and adding betaine had significant interaction on serum albumin content of geese (P≤0.05). 4) At 21 days of age, LPS challenge significantly increased the mRNA relative expression of interleukin-1β (IL-1β) in spleen of geese (P≤0.05), and adding betaine significantly decrease the mRNA relative expression of IL-1β in spleen of geese (P≤0.05). At 28 days of age, LPS challenge and adding betaine had no significant effects on mRNA relative expression of inflammatory factors in spleen of geese (P>0.05). In conclusion, LPS challenge reduce the growth performance and immune performance of geese; and dietary added 0.06% betaine can improve the growth performance of geese, increase the thymus index and reduce the mRNA relative expression of inflammatory factor IL-1β in spleen, and partly effectively alleviate negative impact of geese challenged by LPS. Betaine can alleviate LPS stress during the stress period more obviously than recovery period.

Key words： betaine; geese; lipopolysaccharide; growth performance; inflammatory factors

Cite this article

YANG Jinjin , YANG Zhi , YANG Yu , LING Jingru , XIAO Xia , YANG Haiming , WANG Zhiyue . Effects of Betaine on Growth Performance, Organ Indices, Serum Biochemical Parameters and Spleen Inflammatory Factor mRNA Expression of Geese Challenged by Lipopolysaccharide[J]. Chinese Journal of Animal Nutrition, 2021 , 33(4) : 2044 -2054 . DOI: 10.3969/j.issn.1006-267x.2021.04.024

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