Regulating Effects of Methionine on Milk Protein and Lactose Synthesis in Bovine Mammary Epithelial Cells

ZHAO Yanli; YAN Sumei; GUO Xiaoyu; SHI Binlin; CHEN Lu

doi:10.3969/j.issn.1006-267x.2021.04.027

Chinese Journal of Animal Nutrition >

2021 , Vol. 33 >Issue 4: 2073 - 2082

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.04.027

Regulating Effects of Methionine on Milk Protein and Lactose Synthesis in Bovine Mammary Epithelial Cells

ZHAO Yanli ,
YAN Sumei ,
GUO Xiaoyu ,
SHI Binlin ,
CHEN Lu

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Inner Mongolia Key Laboratory of Animal Nutrition and Feed Science, College of Animal Sciences, Inner Mongolia Agricultural University, Hohhot 010018, China

Received date: 2020-09-18

Online published: 2021-04-15

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Abstract

This study was to detect the effects of methionine (Met) on lactose content, gene expression and protein expression of milk protein and lactose synthesis in bovine mammary epithelial cells (BMECs) to investigate the mechenism of Met regulating milk protein and lactose synthesis. The third generation of BMECs were divided into six groups with six replicates and cultured in medium with 0.13, 0.26, 0.39, 0.52, 0.65 and 0.78 mmol/L Met, respectively. After culturn at 37℃, 5% CO₂ for 48 h, cell viability, ATP content, the relative expression levels of genes involved in milk protein and lactose synthesis, and lactose content were detected. The results showed that compared with 0.13 and 0.78 mmol/L groups, the cell viability in 0.39 to 0.65 mmol/L groups was significantly increased (P<0.05), the relative expression levels of glucose transporter 1 (GLUT1) and α-whey albumin (LALBA) genes were significantly decreased in 0.26 to 0.52 mmol/L groups (P<0.05), and latose content was significantly increased in 0.39 mmol/L group (P<0.05). Conpared with 0.13 to 0.26 mmol/L groups, the relative expression levels of signal transduction and transcription factor 5 (STAT5) and eukaryotic initiation factor 4E (eIF4E) genes and the phosphoralation levels of mammalian target of rapamycin (mTOR) and ribosomal protein S6 kinase 1 (S6K1) in 0.39 to 0.52 mmol/L groups were significantly increased (P<0.05). Compared with the other groups, the ATP content, relative expression levels of β-casein (CSN2), κ-casein (CSN3) and tyrosine kinase 2 (JACK2) genes in 0.52 mmol/L group were significantly increased (P<0.05), but AMP-activated protein kinase (AMPK) phosphoralation level was significantly decreased (P<0.05). The relative expression level of mTOR was significantly greater in 0.26 to 0.52 mmol/L groups than that in 0.65 to 0.78 mmol/L groups (P<0.05). These results demonstrate that the optimal Met concentrations are 0.39 to 0.52 mmo/L for protein and gene expression of milk protein and lactose synthesis in BMECs.

Key words： dairy cow; bovine mammary epithelial cells; methionine; milk protein; lactose

Cite this article

ZHAO Yanli , YAN Sumei , GUO Xiaoyu , SHI Binlin , CHEN Lu . Regulating Effects of Methionine on Milk Protein and Lactose Synthesis in Bovine Mammary Epithelial Cells[J]. Chinese Journal of Animal Nutrition, 2021 , 33(4) : 2073 -2082 . DOI: 10.3969/j.issn.1006-267x.2021.04.027

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