Effects of Subacute Ruminal Acidosis on Rumen Abnormal Metabolites, Rumen Bacterial Flora and Blood Related Indexes of Sheep

HAN Guohao; GAO Xiaosha; DUAN Jinwei; ZHANG Huiqin; ZHENG Yan; MA Linfeng; HE Jinxing; HUO Nairui; PEI Caixia; GU Shaopeng

doi:10.3969/j.issn.1006-267x.2021.04.035

2021 , Vol. 33 >Issue 4: 2158 - 2167

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.04.035

Effects of Subacute Ruminal Acidosis on Rumen Abnormal Metabolites, Rumen Bacterial Flora and Blood Related Indexes of Sheep

HAN Guohao ,
GAO Xiaosha ,
DUAN Jinwei ,
ZHANG Huiqin ,
ZHENG Yan ,
MA Linfeng ,
HE Jinxing ,
HUO Nairui ,
PEI Caixia ,
GU Shaopeng

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1. College of Veterinary Medicine, Shanxi Agricultural University, Taigu 030801, China;
2. College of Animal Science, Shanxi Agricultural University, Taigu 030801, China

Received date: 2020-08-01

Online published: 2021-04-15

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Abstract

In order to provide a theoretical basis for prevention and cure of sheep subacute ruminal acidosis (SARA), the effects of rumen abnormal metabolites, rumen bacterial flora, blood gas and serum biochemical indexes were explored after sheep SARA-induced by gradually increasing the concentrate to forage ratio. Fifty 2-month-old weaned Dorper sheep (♂)×Hu sheep (♀) F1 generations were randomly divided into two groups. The control group (CK group, n=15) was fed a basal diet with concentrate to forage ratio of 50:50, while the SARA induction groups (n=35) were fed diets with gradually increased the concentrate to forage ratios of 50:50, 70:30, 80:20, 90:10 and 100:0 for 7 d at each ratio. Sheep in the SARA induction groups whose rumen fluid pH was between 5.2 and 5.6 for more than 3 h constituted the SARA model group. The rumen fluid was collected at 1, 2, 3, 4, 5, 6 and 8 h after morning feeding on the 7th day of each ratio, and the rumen abnormal metabolites contents and bacterial flora structure were measured. Then, the jugular vein blood was collected from the CK group and the SARA model group, and the blood gas and serum biochemical indexes were measured. The results showed as follows:1) when the concentrate to forage ratio was 100:0, rumen pH of 27 sheep in SARA induction group was 5.2 to 5.6 and lasted for more than 3 h, which were made into the SARA model group. Furthermore, some sheep had diarrhoea, and the rumen fluid was yellowish, smelly and sticky. 2) The Chao1 index of SARA model group was significantly lower than that of CK group (P<0.05), the rumen bacterial flora structure changed, the relative abundance of lactic acid-producing bacteria increased, and the genes related to carbohydrate metabolism in the rumen bacterial flora increased. 3) The contents of lactic acid, histamine and endotoxin in the rumen fluid and blood of SARA model group were significantly higher than those of CK group (P<0.05). 4) The contents of total amount of carbon dioxide, actual bicarbonate and total blood alkali in blood of SARA model group were significantly lower than those of CK group (P<0.05). 5) The serum alanine aminotransferase activity of SARA model group was significantly lower than that of CK group (P<0.05), and both serum biochemical indexes were in the normal range. In summary, the richness of rumen bacterial flora decreases, the bacterial flora structure changes and the lactic acid-producing bacteria relative abundance increases in SARA sheep, the contents of lactic acid, endotoxin and histamine in blood and rumen fluid increase, and metabolic acidosis occurs.

Key words： subacute ruminal acidosis; sheep; rumen bacterial flora; metabolic acidosis; inflammatory response

Cite this article

HAN Guohao , GAO Xiaosha , DUAN Jinwei , ZHANG Huiqin , ZHENG Yan , MA Linfeng , HE Jinxing , HUO Nairui , PEI Caixia , GU Shaopeng . Effects of Subacute Ruminal Acidosis on Rumen Abnormal Metabolites, Rumen Bacterial Flora and Blood Related Indexes of Sheep[J]. Chinese Journal of Animal Nutrition, 2021 , 33(4) : 2158 -2167 . DOI: 10.3969/j.issn.1006-267x.2021.04.035

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