Effects of Yeast Hydrolyzate on Growth, Antioxidant, Immune and Intestinal Flora of Juvenile Macrobrachium nipponense

  • XIONG Yunfeng ,
  • WU Jiawen ,
  • ZHOU Dongsheng ,
  • ZHENG Jinxian ,
  • QIU Tian ,
  • LI Qiulin ,
  • KONG Youqin ,
  • SHAO Xianping ,
  • YE Jinyun ,
  • DING Zhili
Expand
  • Zhejiang Provincial Key Laboratory of Aquatic Resources Conservation and Development, Key Laboratory of Aquatic Animal Genetic Breeding and Nutrition of Chinese Academy of Fishery Sciences, College of Life Science, Huzhou University, Huzhou 313000, China

Received date: 2020-09-17

  Online published: 2021-04-15

Supported by

 

Abstract

The purpose of this study was to study the effects of yeast hydrolyzate (YH) on growth, antioxidation, immunity and intestinal flora of Juvenile Macrobrachium nipponense. Five experimental diets were obtained by adding 0 (YH1), 0.5% (YH2), 1.0% (YH3), 2.0% (YH4) and 4.0% (YH5) YH to the basal diet with protein level of 37% and lipid level of 8%. A total of 1 000 Juvenile M. nipponense with an average weight of (0.104±0.003) g was randomly divided into 5 groups, each with 4 parallels (50 fish/parallel), and the feeding experiment lasted for 8 weeks. The results showed as follows:1) the survival rate of M. nipponense was not affected by the different YH levels in the diet (P>0.05), but when the YH level in the diet was 1.0%, the weight gain rate and specific growth rate of M. nipponense were significantly higher than those in YH1 group (P<0.05) and reached the highest. 2) Compared with YH1 and YH5 groups, the content of malondialdehyde (MDA) in hepatopancreas in YH3 and YH4 groups decreased significantly (P<0.05). The total antioxidant capacity (T-AOC) in hepatopancreas in YH2, YH3 and YH4 groups was significantly higher than that in YH1 and YH5 groups (P<0.05). The activity of superoxide dismutase (SOD) in YH4 group was significantly higher than that in YH1, YH2 and YH5 groups (P<0.05). Glutathione peroxidase (GSH-Px) activity in YH5 group was significantly lower than that in other groups (P<0.05). Nitric oxide (NO) content and nitric oxide synthase (NOS) activity in YH5 group were significantly higher than those in other groups (P<0.05). 3) At the phylum level, there were 8 dominant bacteria in the intestinal tract, which were Proteobacteria, Firmicutes, Tenericutes, Bacteroidetes, Cyanobacteria, Actinobacteria, Chlamydiae and Planctomycetes. At the genus level, there were 11 dominant bacteria in the intestinal tract, which were Pseudomonas, Shewanella, Aeromonas, Reyranella, Chitinibacter, Acinetobacter, Flavobacterium, Mycobacterium, Rhodobacter, Stenotrophomonas and Legionella. No beneficial bacteria at the genus level increased due to the addition of YH (P<0.05), but adding a certain amount of YH could inhibit the growth of pathogenic bacteria and potential pathogenic bacteria such as Shewanella, Aeromonas, Acinetobacter and Legionella. It can be seen that adding 1.0% YH to the diet can promote the growth of M. nipponense. When the addition level reaches 1.0% to 2.0%, the antioxidant capacity of prawns can be improved. When the addition level reaches 4.0%, it can significantly change the intestinal flora of prawns and improve the nonspecific immunity of prawns. According to growth performance and antioxidant capacity, the addition amount of YH in the diet is suggested to be 1.0%. According to the immune performance, the amount of YH in the diet is suggested to be 4.0%.

Cite this article

XIONG Yunfeng , WU Jiawen , ZHOU Dongsheng , ZHENG Jinxian , QIU Tian , LI Qiulin , KONG Youqin , SHAO Xianping , YE Jinyun , DING Zhili . Effects of Yeast Hydrolyzate on Growth, Antioxidant, Immune and Intestinal Flora of Juvenile Macrobrachium nipponense[J]. Chinese Journal of Animal Nutrition, 2021 , 33(4) : 2199 -2212 . DOI: 10.3969/j.issn.1006-267x.2021.04.039

References

[1] 刘宝良,雷霁霖,贾睿,等.养殖密度对鱼类福利影响研究进展[J].中国工程科学,2014,16(9):100-105. LIU B L,LEI J L,JIA R,et al.A review:the influence of stocking density on fish welfare[J].China Engineering Science,2014,16(9):100-105.(in Chinese)
[2] LIN W,LI L,CHEN J,et al.Long-term crowding stress causes compromised nonspecific immunity and increases apoptosis of spleen in grass carp (Ctenopharyngodon idella)[J].Fish & Shellfish Immunology,2018,80:540-545.
[3] AMORIM M,PEREIRA J O,GOMES D,et al.Nutritional ingredients from spent brewer's yeast obtained by hydrolysis and selective membrane filtration integrated in a pilot process[J].Journal of Food Engineering,2016,185:42-47.
[4] 贺淼,黄鑫,陈中平,等.酵母水解物的消化吸收及营养作用[J].中国饲料,2014(9):38-41. HE M,HUANG X,CHEN Z P,et al.The digestion and absorption of yeast hydrolyzate and its nutritional value[J].Chinese Feed,2014(9):38-41.(in Chinese)
[5] 周慧琦,李彪,燕富永,等.酵母水解物对母猪生产性能和血液生理生化指标的影响[J].中国饲料,2015(3):38-41. ZHOU H Q,LI B,YAN F Y,et al.Effects of dietary supplement with autoolyzed yeast on production performance and blood biochemical indicators of sows[J].Chinese Feed,2015(3):38-41.(in Chinese)
[6] JIN M,XIONG J,ZHOU Q C,et al.Dietary yeast hydrolysate and brewer's yeast supplementation could enhance growth performance,innate immunity capacity and ammonia nitrogen stress resistance ability of Pacific white shrimp (Litopenaeus vannamei)[J].Fish & Shellfish Immunology,2018,82:121-129.
[7] ABDEL-TAWWAB M,ABDEL-RAHMAN A M,ISMAEL N E M.Evaluation of commercial live bakers' yeast,Saccharomyces cerevisiae as a growth and immunity promoter for fry Nile tilapia,Oreochromis niloticus (L.) challenged in situ with Aeromonas hydrophila[J].Aquaculture,2008,280(1/2/3/4):185-189.
[8] ZHANG R Q,JIANG Y,ZHOU L,et al.Effects of dietary yeast extract supplementation on growth,body composition,non-specific immunity,and antioxidant status of Chinese mitten crab (Eriocheir sinensis)[J].Fish & Shellfish Immunology,2019,86:1019-1025.
[9] 柳茜,杨文娇,吴振,等.酵母水解物对大菱鲆幼鱼非特异性免疫及抗应激能力的影响[J].饲料工业,2015,36(18):33-37. LIU Q,YANG W J,WU Z,et al.Effects of yeast hydrolysate on non-specific immunity and anti-stress abilities of juvenile turbot(Scophthalmus maximus)[J].Feed Industry,2015,36(18):33-37. (in Chinese)
[10] CHOCT M.Managing gut health through nutrition[J].British Poultry Science,2009,50(1):9-15.  
[11] HOOPER L V,MACPHERSON A J.Immune adaptations that maintain homeostasis with the intestinal microbiota[J].Nature Reviews Immunology,2010,10(3):159-169.  
[12] WONG S D,WALDROP T,SUMMERFELT S,et al.Aquacultured rainbow trout (Oncorhynchus mykiss) possess a large core intestinal microbiota that is resistant to variation in diet and rearing density[J].Applied and Environmental Microbiology,2013,79(16):4974-4984.  
[13] CARBALLO C,PINTO P I S,MATEUS A P,et al.Yeast β-glucans and microalgal extracts modulate the immune response and gut microbiome in Senegalese sole (Solea senegalensis)[J].Fish & Shellfish Immunology,2019,92:31-39.
[14] SUN S M,FU H T,GU Z M,et al.Effects of stocking density on the individual growth and differentiation of the oriental river prawn Macrobrachium nipponense (De Haan,1849) (Caridea:Palaemonidae)[J].Journal of Crustacean Biology,2016,36(6):769-775.  
[15] WANG W N,WANG A L,ZHANG Y J,et al.Effects of nitrite on lethal and immune response of Macrobrachium nipponense[J].Aquaculture,2004,232(1/2/3/4):679-686.
[16] 管越强,王慧春,李利.硫化物胁迫对日本沼虾呼吸代谢和能量代谢酶的影响[J].生态环境学报,2009,18(6):2017-2022. GUANG Y Q,WANG H C,LI L.Effects of sulphide on the enzyme of respiratory metabolism and energy metabolism of Macrobrachium nipponense[J].Ecology and Environmental Sciences,2009,18(6):2017-2022. (in Chinese)
[17] JAMWAL A,LEMIRE D,DRIESSNACK M,et al.Interactive effects of chronic dietary selenomethionine and cadmium exposure in rainbow trout (Oncorhynchus mykiss):a preliminary study[J].Chemosphere,2018,197:550-559.
[18] 郑纯纯,李小雨,聂欢,等.慢性铅胁迫对日本沼虾硫氧还蛋白和热应激蛋白系统基因表达及肠道菌群的影响[J].动物营养学报,2019,31(11):5378-5390. ZHENG C C,LI X Y,NIE H,et al.Effects of chronic lead exposure on expressions of thioredoxin and heat shock protein system genes and intestinal microbiota in Macrobrachium nipponense[J].Chinese Journal of Animal Nutrition,2019,31(11):5378-5390. (in Chinese)
[19] ANDRIAMIALINIRINA H J T,IRM M,TAJ S,et al.The effects of dietary yeast hydrolysate on growth,hematology,antioxidant enzyme activities and non-specific immunity of juvenile Nile tilapia,Oreochromis niloticus[J].Fish & Shellfish Immunology,2020,101:168-175.
[20] 高敏敏.酵母源饲料添加剂对异育银鲫生长、健康、氧化油脂损伤修复和抗病力的影响[D].硕士学位论文.苏州:苏州大学,2019. GAO M M.Effects of yeast source feed additives on growth,health,repairing of oxidized oil injury and disease resistance of allogynogenetic silver crucian carp (Carassius auratus gibelio ♀×Carassius auratus gibelio)[D].Master's Thesis.Soochow:Soochow University,2019. (in Chinese)
[21] 孙飞,吕昊,郁浓,等.饲料酵母培养物提高异育银鲫的抗氧化、免疫能力及CyHV-2病毒攻毒保护能力[J].中国粮油学报,2020,35(1):97-106. SUN F,LU H,YU N,et al.Diet yeast culture improves the ability to anti-oxidation,immunity and protection against CyHV-2 virus of the gibel carp[J].Chinese Journal of Cereals and Oils,2020,35(1):97-106. (in Chinese)
[22] 杨军,冯德品,董舰峰,等.免疫增强剂对齐口裂腹鱼生长性能和免疫功能的影响[J].水生态学杂志,2019,40(5):78-83. YANG J,FENG D P,DONG J F,et al.Effect of immunopotentiator on growth performance and immune function of Schizothorax prenanti[J].Journal of Water Ecology,2019,40(5):78-83. (in Chinese)
[23] 曾本和,杨文娇,吴振,等.酵母水解物对加州鲈幼鱼生长性能及免疫酶指标的影响[J].饲料工业,2016,37(14):11-15. ZENG B H,YANG W J,WU Z,et al.Effects of yeast hydrolyzate on growth and immune enzyme index of juvenile Micropterus salmoides[J].Feed Industry,2016,37(14):11-15. (in Chinese)
[24] GONG Y L,YANG F,HU J P,et al.Effects of dietary yeast hydrolysate on the growth,antioxidant response,immune response and disease resistance of largemouth bass (Micropterus salmoides)[J].Fish & Shellfish Immunology,2019,94:548-557.
[25] 陈晓瑛,王国霞,邹青,等.酵母产品对凡纳滨对虾的诱食效果及生长性能的影响[J].饲料工业,2020,41(12):42-49. CHEN X Y,WANG G X,ZOU Q,et al.Effects of the yeast products on feed attractants and growth performance of Litopenaeus vannamei[J].Feed Industry,2020,41(12):42-49. (in Chinese)
[26] ZHU M,WU S J.The growth performance and nonspecific immunity of loach Paramisgurnus dabryanus as affected by dietary β-1,3-glucan[J].Fish & Shellfish Immunology,2018,83:368-372.
[27] 王际英,姜柯君,夏斌,等.小肽对星斑川鲽幼鱼消化酶活性、抗氧化能力和生化组成的影响[J].中国水产科学,2014,21(6):1154-1164. WANG J Y,JIANG K J,XIA B,et al.Effects of small dietary peptides on digestive enzyme activity,antioxidative capability,and biochemical composition in tissues of juvenile starry flounder Platichthys stellatus[J].Chinese Fisheries Science,2014,21(6):1154-1164. (in Chinese)
[28] WANG T,CHENG Y Z,CHEN X Y,et al.Effects of small peptides,probiotics,prebiotics,and synbiotics on growth performance,digestive enzymes,and oxidative stress in orange-spotted grouper,Epinephelus coioides,juveniles reared in artificial seawater[J].Chinese Journal of Oceanology and Limnology,2017,35(1):89-97.  
[29] ZHU M,WU S J.The growth performance and nonspecific immunity of loach Paramisgurnus dabryanus as affected by dietary β-1,3-glucan[J].Fish & Shellfish Immunology,2018,83:368-372.
[30] YANG X Y,CHI S Y,TAN B P,et al.Yeast hydrolysate helping the complex plant proteins to improve the growth performance and feed utilization of Litopenaeus vannamei[J].Aquaculture Reports,2020,17:100375.
[31] 聂琴,周小辉,杨凡,等.酵母水解物与乌贼副产品的营养价值比较[J].中国饲料,2014(11):42-43. NIE Q,ZHOU X H,YANG F,et al.Comparison of nutrition value in yeast hydrolysate and squid visceral meal[J].Chinese Feed,2014(11):42-43. (in Chinese)
[32] OLSÉN K H,LUNDH T.Feeding stimulants in an omnivorous species,crucian carp Carassius carassius (Linnaeus 1758)[J].Aquaculture Reports,2016,4:66-73
[33] FATTMAN C L,SCHAEFER L M,OURY T D.Extracellular superoxide dismutase in biology and medicine[J].Free Radical Biology and Medicine,2003,35(3):236-256.  
[34] 董亮,何永志,王远亮,等.超氧化物歧化酶(SOD)的应用研究进展[J].中国农业科技导报,2013,15(5):53-58. DONG L,HE Y Z,WANG Y L,et al.Research progress on application of superoxide dismutase (SOD)[J].Journal of Agricultural Science and Techology,2013,15(5):53-58. (in Chinese)
[35] 蒋敏敏,叶金云,邵仙萍,等.酵母水解物对青鱼幼鱼生长性能、肌肉品质及肝胰脏抗氧化指标和组织形态的影响[J].动物营养学报,2020,32(5):2326-2341. JIANG M M,YE J Y,SHAO X P,et al.Effects of yeast hydrolysate on growth performance,muscle quality,hepatopancreases antioxidant indexes and tissue morphology of juvenile black carp (Mylopharyngodon piceus)[J].Chinese Journal of Animal Nutrition,2020,32(5):2326-2341. (in Chinese)
[36] 彭凯,孙育平,王国霞,等.5种酵母源物质对草鱼生长性能、肌肉品质和血清指标的影响[J].饲料工业,2018,39(20):16-22. PENG K,SUN Y P,WANG G X,et al.Effects of 5 yeast based additives on growth performance,meat quality and serum parameters of Ctenopharyngodon idella[J].Feed Industry,2018,39(20):16-22. (in Chinese)
[37] 丁斌,邢爽,刘露,等.酿酒酵母在动物生产中的应用研究进展[J].饲料研究,2019,42(7):114-116. DING B,XING S,LIU L,et al.Research progress on application of Saccharomyces cerevisiae in animal production[J].Feed Research,2019,42(7):114-116. (in Chinese)
[38] 曹俊明,吴春玉,黄燕华,等.β-葡聚糖对花鲈免疫和抗氧化指标的影响[J].水产科学,2015,34(1):1-7. CAO J M,WU C Y,HUANG Y H,et al.Effects of dietary β-glucan on immunity and antioxidation indices in japanese seabass Lateolabrax japonicus[J].Fisheries Science,2015,34(1):1-7. (in Chinese)
[39] 李会峰.水飞蓟素、菊粉和β-葡聚糖对缓解凡纳滨对虾低盐应激的效应研究[D].硕士学位论文.上海:华东师范大学,2019. LI H F.Benificial effects of silymarin,inulin and β-glucan in relieving low-salinity caused stress in Litopenaeus vannamei[D].Master's Thesis.Shanghai:East China Normal University,2019.
[40] 王广军,谢骏,余德光,等.一氧化氮及一氧化氮合酶在水生动物免疫系统中的研究进展[J].水产科技,2009(3):1-6,13. WANG G J,XIE J,YU D G,et al.The advances of nitric oxide and nitric oxide synthase in immune system of aquatic animals[J].Fisheries Science & Technology,2009(3):1-6,13. (in Chinese)
[41] FRANCHINI A,FONTANILI P,OTTAVIANI E.Invertebrate immunocytes:relationship between phagocytosis and nitric oxide production[J].Comparative Biochemistry and Physiology Part B:Biochemistry and Molecular Biology,1995,110(2):403-407.  
[42] 张国启.壳寡糖对黄河鲤生产性能、免疫指标和肠道菌群影响的研究[D].硕士学位论文.郑州:河南工业大学,2012. ZHANG G Q.Effect of chitosan oligosaccharide on growth performance,immunity parameters and intestinal flora of the yellow river carp[D].Master's Thesis.Zhengzhou:Henan University of Technology,2012. (in Chinese)
[43] RAHIMNEJAD S,YUAN X Y,LIU W B,et al.Evaluation of antioxidant capacity and immunomodulatory effects of yeast hydrolysates for hepatocytes of blunt snout bream (Megalobrama amblycephala)[J].Fish & Shellfish Immunology,2020,106:142-148.
[44] COSCELLI G,BERMÚDEZ R,RONZA P,et al.Immunohistochemical study of inducible nitric oxide synthase and tumour necrosis factor alpha response in turbot (Scophthalmus maximus) experimentally infected with Aeromonas salmonicida subsp. salmonicida[J].Fish & Shellfish Immunology,2016,56:294-302.
[45] ANDRIALINIRINA H J T,IRM M,TAJ S,et al.The effects of dietary yeast hydrolysate on growth,hematology,antioxidant enzyme activities and non-specific immunity of juvenile Nile tilapia,Oreochromis niloticus[J].Fish & Shellfish Immunology,2020,101:168-175.
[46] 陈超然,陈萱,陈昌福,等.酵母β-葡聚糖对受免异育银鲫免疫应答的增强作用[J].华中农业大学学报,2003,22(4):380-384. CHEN C R,CHEN X,CHEN C F,et al.Enhancement of immune response in gibel carb (Carassius auratus gibelio) inoculated with aeromonas hydrophila bacterin using yeast β-glucan[J].Journal of Huazhong Agricultural University,2003,22(4):380-384. (in Chinese)
[47] KAZUŃ B,MALACZEWSKA J,KAZUŃ K,et al.Dietary administration of β-1,3/1,6-glucan and Lactobacillus plantarum improves innate immune response and increases the number of intestine immune cells in roach (Rutilus rutilus)[J].BMC Veterinary Research,2020,16:216.
[48] JIANG C Y,WANG P,LIU S S,et al.Administration of yeast glucan on immunity of offspring in turbot Scophthalmus maximus:a trans-generational immune-enhancing effect[J].Journal of Ocean University of China,2019,18(4):997-1003.  
[49] MEENA D K,DAS P,KUMAR S,et al.Beta-glucan:an ideal immunostimulant in aquaculture (a review)[J].Fish Physiology and Biochemistry,2013,39(3):431-457.  
[50] 李改娟,赵全东,高娜,等.鱼类健康与肠道微生物关系研究进展[J].河北渔业,2020(8):56-58. LI G J,ZHAO Q D,GAO N,et al.Research progress on the relationship between gut microbes and fish health[J].Hebei Fishery,2020(8):56-58. (in Chinese)
[51] FOYSAL M J,ALAM M,MOMTAZ F,et al.Dietary supplementation of garlic (Allium sativum) modulates gut microbiota and health status of tilapia (Oreochromis niloticus) against Streptococcus iniae infection[J].Aquaculture Research,2019,50(8):2107-2116.  
[52] GAJARDO K,JARAMILLO-TORRES A,KORTNER T M,et al.Alternative protein sources in the diet modulate microbiota and functionality in the distal intestine of atlantic salmon (Salmo salar)[J].Applied and Environmental Microbiology,2017,83(5):e02615-16.
[53] WANG C,SUN G X,LI S S,et al.Intestinal microbiota of healthy and unhealthy Atlantic salmon Salmo salar L. in a recirculating aquaculture system[J].Journal of Oceanology and Limnology,2017,36(2):414-426.
[54] DEHLER C E,SECOMBES C J,MARTIN S A M.Environmental and physiological factors shape the gut microbiota of Atlantic salmon parr (Salmo salar L.)[J].Aquaculture,2017,467:149-157.
[55] 陈一铭,顾泽茂,李莉娟,等.白斑综合征病毒感染对克氏原螯虾肠道菌群的影响[J].华中农业大学学报,2020,39(2):40-46. CHEN Y M,GU Z M,LI L J,et al.Effect of white spot syndrome virus (WSSV) infection on intestinal flora composition of Procambarus clarkii[J].Journal of Huazhong Agricultural University,2020,39(2):40-46. (in Chinese)
[56] 韩凤禄.大豆抗原蛋白对中华绒螯蟹生长性能和肠道健康的影响及其改善对策[D].博士学位论文.上海:华东师范大学,2020. HAN F L.Effect of soybean antigen protein on growth performance and intestinal health of Chinese mitten crab (Eriocheir sinensis) and reguiation strategy[D].Ph.D.Thesis.Shanghai:East China Normal University,2020. (in Chinese)
[57] 孙振丽,宣引明,张皓,等.南美白对虾养殖环境及其肠道细菌多样性分析[J].中国水产科学,2016,23(3):594-605. SUN Z L,XUAN Y M,ZHANG H,et al.Bacterial diversity of Penaeus vannamei boone intestine and aquaculture environment[J].Chinese Fisheries Science,2016,23(3):594-605. (in Chinese)
[58] 王闻卿,朱林英,郝莉鹏,等.气单胞菌研究概况[J].疾病监测,2016,31(7):591-597. WANG W Q,ZHU LY,HAO L P,et al.Progress in research of Aeromonas[J].Disease Surveillance,2016,31(7):591-597. (in Chinese)
[59] 商宝娣,杨星,李正友,等.希瓦氏菌的研究进展[J].福建农业,2015(7):152-154. SHANG B D,YANG X,LI Z Y,et al.Research progress of Shewanella[J].Fujian Agriculture,2015(7):152-154. (in Chinese)
[60] 刘志刚,卢迈新,可小丽,等.尼罗罗非鱼肠道及养殖环境中菌群结构与链球菌病的相关性[J].水产学报,2018,42(10):1635-1647. LIU Z G,LU M X,KE X L,et al.Correlation between microbial structure in intestine tract and aquaculture environment of tilapia (Oreochromis niloticus) and Streptococcosis[J].Journal of Fisheries of China,2018,42(10):1635-1647. (in Chinese)
[61] 尹景峰.军团菌的研究进展[J].现代农业,2017(10):77-81. YIN J F.Research progress of Legionella[J].Modern Agriculture,2017(10):77-81. (in Chinese)
[62] ZHOU M,LIANG R S,MO J F,et al.Effects of brewer's yeast hydrolysate on the growth performance and the intestinal bacterial diversity of largemouth bass (Micropterus salmoides)[J].Aquaculture,2018,484:139-144.
[63] 赵月季,郭海朋,张德民.不同养殖模式对凡纳滨对虾肠道菌群的影响[J/OL].水产学报,2020:1-11.(2020-09-02)[2020-09-06].http://kns.cnki.net/kcms/detail/31.1283.S.20200902.1408.005.html. ZHAO Y J,GUO H P,ZHANG D M.Effects of different culture patterns on the intestinal of Litopenaeus vannamei[J/OL].Journal of Fisheries of China,2020:1-11.(2020-09-02)[2020-09-06].http://kns.cnki.net/kcms/detail/31.1283.S.20200902.1408.005.html. (in Chinese)
[64] ZHANG H L,DING Q W,WANG A R,et al.Effects of dietary sodium acetate on food intake,weight gain,intestinal digestive enzyme activities,energy metabolism and gut microbiota in cultured fish:Zebrafish as a model[J].Aquaculture,2020,523:735188.
[65] 孟现尧,朱晓振,贾晨晨,等.菊芋全粉与地衣芽孢杆菌对凡纳滨对虾生长性能、免疫能力及肠道菌群变化的影响[J].中国饲料,2020(15):77-83. MENG X Y,ZHU X Z,JIA C C,et al.Effects of Jerusalem artichoke powder and Bacillus licheniformis on growth performance,immunity and intestinal microorganism of Litopenaeus vannamei[J].Chinese Feed,2020(15):77-83. (in Chinese)
[66] AYIKU S,SHEN J F,TAN B P,et al.Effects of dietary yeast culture on shrimp growth, immune response, intestinal health and disease resistance against Vibrio harveyi[J].Fish & Shellfish Immunology,2020,102:286-295.
[67] 孔祎頔,田佳鑫,陈秀梅,等.水产动物益生菌及其对肠道菌群影响的研究进展[J].中国畜牧杂志,2019,55(1):29-33. KONG Y Q,TIAN J X,CHEN X M,et al.Aquatic animal probiotics and its effects on intestinal flora[J].Chinese Journal of Animal Husbandry,2019,55(1):29-33. (in Chinese)
[68] KIM D H,BRUNT J,AUSTIN B.Microbial diversity of intestinal contents and mucus in rainbow trout (Oncorhynchus mykiss)[J].Journal of Applied Microbiology,2017,102(6):1654-1664.
[69] ZHAXI Y P,MENG X Q,WANG W H,et al.Duan-Nai-An,a yeast probiotic,improves intestinal mucosa integrity and immune function in weaned piglets[J].Scientific Reports,2020,10(1):4556.
[70] 杜宗君,苏健,巫红萍,等.甘露寡糖对泥鳅生产性能、肠道菌群和非特异性免疫功能的影响[J].四川农业大学学报,2019,37(1):129-133. DU Z J,SU J,WU H P,et al.Effect of mannose oligosaccharides on the production performance,intestinal flora and non-specific immune function of Misgurnus anguillicaudatus[J].Journal of Sichuan Agricultural University,2019,37(1):129-133. (in Chinese)
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