Effects of Biofilm and Planktonic Lactobacillus plantarum LR-39 on Serum Immune Indexes and Intestinal Microbiota Structure of Beagle Dogs

CHEN Zedong; GUO Shuyu; ZHU Jianping; LI Zihan; LU Wei; LIU Lei

doi:10.3969/j.issn.1006-267x.2021.04.040

2021 , Vol. 33 >Issue 4: 2213 - 2223

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.04.040

Effects of Biofilm and Planktonic Lactobacillus plantarum LR-39 on Serum Immune Indexes and Intestinal Microbiota Structure of Beagle Dogs

CHEN Zedong ,
GUO Shuyu ,
ZHU Jianping ,
LI Zihan ,
LU Wei ,
LIU Lei

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1. Department of Pet Science and Technology, Jiangsu Agri-Animal Husbandry Vocational College, Taizhou 225300, China;
2. School of Food and Biological Engineering, Xihua University, Chengdu 610039, China

Received date: 2020-09-02

Online published: 2021-04-15

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Abstract

This experiment was to compare the effects of biofilm and planktonic Lactobacillus plantarum LR-39 on serum immune indexes and intestinal microbiota structure of beagle dogs. The planktonic and biofilm Lactobacillus plantarum LR-39 were prepared, and the survival rates of two different biological states of Lactobacillus plantarum LR-39 treated with acid and bile salt environment were compared by colony counting method. Fifteen 3.5-month-old beagle dogs were selected and randomly divided into biofilm group, planktonic group and control group,each group contained 5 dogs. The biofilm group and the planktonic group were gavaged with 1 mL (1×10⁹ CFU/mL) biofilm and planktonic Lactobacillus plantarum LR-39 after feeding dog food every morning, respectively, and the control group was gavaged with equal volume phosphate buffered saline (PBS), continuous gavage 28 days. The contents of immunoglobulin G (IgG), secretory immunoglobulin A (sIgA), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α) in serum were detected, and intestinal microbiota were detected by 16S rDNA high throughput sequencing analysis. The results showed as follows:1) after treated with acid and bile salt environment, the survival rate of biofilm Lactobacillus plantarum LR-39 was significantly higher than planktonic Lactobacillus plantarum LR-39 (P<0.05 or P<0.01). 2) Compared with the control group, the contents of IgG and sIgA in serum of biofilm group were significantly increased (P<0.01); compared with the planktonic group, the serum sIgA content of biofilm group was significantly increased (P<0.01), and the serum IL-6 content was tend to decrease (0.05≤P<0.10); compared with the control group, the contents of IgG, sIgA, IL-6 and TNF-α in serum of planktonic group were no significant difference (P>0.05). 3) The α diversity analysis showed that compared with the control group, the Observed species index, Chao index and Ace index of biofilm group and planktonic group were increased, the Shannon index of biofilm group was increased, and the Simpson index was decreased; the Shannon index of planktonic group was decreased, and the Simpson index was increased. The principal coordinate analysis (PCoA) results showed that there was obvious clustering among three groups of samples. 4) At the phylum level, compared with the control group, the relative abundances of Firmicutes in intestinal tract of biofilm group were increased, and the relative abundances of Bacteroidetes, Fusobacteria, Proteobacteria and Actinobacteria in intestinal tract were decreased; the relative abundances of Bacteroidetes and Fusobacteria in intestinal tract of planktonic group were increased, and the relative abundances of Firmicutes and Actinobacteria in intestinal tract were decreased. 5) At the genus level, compared with the control group, the relative abundances of Bifidobacterium, Lactobacillus, Allobaculum and Peptostreptococcus in intestinal tract of biofilm group were increased; the relative abundances of Lactobacillus, Peptostreptococcus, Fusobacterium, Prevotella, Sutterella in intestinal tract of planktonic group were increased. 6) LEfSe analysis showed that the dominant genus of biofilm group was Lactobacillus, the dominant genus of planktonic group was Fusobacterium, and the dominant genera of control group included Collinsella, Catenibacterium and Eubacterium. 7) Spearman correlation analysis showed that there was no significant correlation between the contents of IgG, sIgA, IL-6 and TNF-α and the relative abundance of intestinal enriched flora (P>0.05). In conclusion, biofilm and planktonic Lactobacillus plantarum LR-39 have different effects on intestinal microbiota structure of dogs, the biofilm Lactobacillus plantarum LR-39 has stronger acid and bile salt resistance, and can effectively adjust the intestinal microbiota structure and improve the body immune level.

Key words： Lactobacillus plantarum; biofilm; dogs; immune indexes; intestinal microbiota; high-throughput sequencing

Cite this article

CHEN Zedong , GUO Shuyu , ZHU Jianping , LI Zihan , LU Wei , LIU Lei . Effects of Biofilm and Planktonic Lactobacillus plantarum LR-39 on Serum Immune Indexes and Intestinal Microbiota Structure of Beagle Dogs[J]. Chinese Journal of Animal Nutrition, 2021 , 33(4) : 2213 -2223 . DOI: 10.3969/j.issn.1006-267x.2021.04.040

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