Effects of Diets with Different Protein Levels on Body Condition, Serum Biochemical Indexes, Short Chain Fatty Acid Concentrations in Feces and Gut Microbiota Structure of Obese Dogs

  • LU Jiang ,
  • ZHU Daoxian ,
  • LIU Li ,
  • HAO Fuxing ,
  • WU Zhi ,
  • LU Jinye ,
  • LU Wei ,
  • LIU Jing
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  • 1. Department of Pet Science and Technology, Jiangsu Agri-Animal Husbandry Vocational College, Taizhou 225300, China;
    2. Department of Animal Medicine, Jiangsu Agri-Animal Husbandry Vocational College, Taizhou 225300, China;
    3. Jiangsu Provincial Key Laboratory of Veterinary Bio-Pharmaceutical High Technology Research, Taizhou 225300, China

Received date: 2020-09-07

  Online published: 2021-04-15

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Abstract

The aim of this study was to evaluate the effects of diets with different protein levels on body condition, serum biochemical indexes, short chain fatty acid concentrations in feces and gut microbiota structure of obese dogs. Thirty obese Labrador dogs were randomly and equally divided into three groups:low protein group (LP group), dogs in this groups were fed low protein diet which had 20.58% crude protein; medium protein group (MP group), dogs in this groups were fed medium protein diet which had 27.77% crude protein; high protein group (HP group), dogs in this groups were fed high protein diet which had 39.04% crude protein. The experimental period was 45 days. The results showed as follows:1) compared with LP group and MP group, the body weight, body fat rate, and the contents of serum triglyceride (TG), total cholesterol (TC) and low density lipoprotein (LDL) in HP group were significantly decreased (P<0.05). 2) Compared with LP group and MP Group, the concentrations of acetic acid and total short chain fatty acids in feces of HP group were significantly decreased (P<0.05 or P<0.01), and butyric acid concentration was significantly increased (P<0.05). Body fat rate was positively correlated with the concentrations of acetic acid and total short chain fatty acids in feces, but negatively correlated with butyric acid concentration. 3) The Simpson index of gut microbiota and the relative abundance of Firmicutes were significantly increased (P<0.05), while the relative abundance of Bacteroides and the ratio of Bacteroides/Firmicutes were decreased in HP group compared with those in LP group and MP group (P<0.05). At genus level, Clostridium, Ruminococcus, Faecalibacterium and so on were mainly enriched in HP group. The gene functions of gut microbiota in HP group were mainly concentrated in seven KEGG pathways (level 3), including flavone and flavonol biosynthesis, tryptophan metabolism, limonene and pinene degradation and so on. In summary, high protein diet can regulate the gut microflora structure and short chain fatty acids metabolism, and promote the effect of weight loss and lipid reduction in obese dogs.

Cite this article

LU Jiang , ZHU Daoxian , LIU Li , HAO Fuxing , WU Zhi , LU Jinye , LU Wei , LIU Jing . Effects of Diets with Different Protein Levels on Body Condition, Serum Biochemical Indexes, Short Chain Fatty Acid Concentrations in Feces and Gut Microbiota Structure of Obese Dogs[J]. Chinese Journal of Animal Nutrition, 2021 , 33(4) : 2224 -2234 . DOI: 10.3969/j.issn.1006-267x.2021.04.041

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