Analysis of Differentially Expressed Genes for Adipogenic Differentiation of Yanbian Bovine Skeletal Muscle Satellite Cells Induced by Oleic Acid Based on Transcriptome Sequencing

SUN Jianfu; SUN Bin; ZHANG Junfang; WANG Ying; CUI Yan; LI Qiang; CHOI Seong; SHIN Jong; LI Xiangzi

doi:10.3969/j.issn.1006-267x.2021.04.045

2021 , Vol. 33 >Issue 4: 2263 - 2277

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.04.045

Analysis of Differentially Expressed Genes for Adipogenic Differentiation of Yanbian Bovine Skeletal Muscle Satellite Cells Induced by Oleic Acid Based on Transcriptome Sequencing

SUN Jianfu ,
SUN Bin ,
ZHANG Junfang ,
WANG Ying ,
CUI Yan ,
LI Qiang ,
CHOI Seong ,
SHIN Jong ,
LI Xiangzi

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1. Northeast Cold Region Beef Cattle Science and Technology Innovation Ministry of Education Engineering Research Center, Yanbian University, Yanji 133002, China;
2. College of Animal Life Sciences, Chungbuk National University, Cheongju 361-763, South Korea;
3. College of Animal Life Sciences, Gangwon National University, Chuncheon 200-701, South Korea

Received date: 2020-10-22

Online published: 2021-04-15

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Abstract

This study aimed to study the molecular regulation mechanism of oleic acid (OA)-induced adipogenic differentiation of Yanbian bovine skeletal muscle satellite cells. The skeletal muscle satellite cells isolated from the 12-day-old Yanbian cattle were cultured in vitro, and different OA concentrations were added to induce differentiation media for differentiation. The experiment set up a blank control group[CON group, 5% horse serum (HS)], 3 OA induction groups:OAL group (5% HS+50 μmol/L), OAM group (5% HS+100 μmol/L), OAH group (5% HS+200 μmol/L). After 96 h of induction, transcriptome sequencing and analysis of Yanbian bovine skeletal muscle satellite cells were performed. The results showed as follows:a total of 13 951 single genes were obtained. The differentially expressed genes of the 4 groups were compared and found that compared with the CON group, the OAL group had 3 412 differentially expressed genes, the OAM group had 1 045 differentially expressed genes, and there were 1 428 differentially expressed genes in OAH group, and there were a total of 278 differentially expressed genes between each groups. GO enrichment analysis showd that differential genes were involved in a variety of biological processes, including metabolic processes, cellular processes, and biological regulation processes. In terms of molecular functions, most gene functions were related to binding activity, transcription regulation activity, and catalytic activity; in the category of cell components, most of the genes were enriched in cells, organelles, membranes and so on. KEGG enrichment analysis showed that the main enrichment pathways of differentially expressed genes were AMP-activated protein kinase signaling pathway, peroxisome proliferators-activated receptors signaling pathway, fatty acid metabolism pathway, fatty acid degradation pathway, etc. The results of real-time PCR showed that the five genes were consistent with the transcriptome sequencing results, which indicated the reliability of the sequencing results. This experiment completes the transcriptome sequencing analysis of the adipogenic differentiation of Yanbian bovine skeletal muscle satellite cells induced by OA, obtained functional annotation information of different genes, and initially revealed the potential genes and pathways of OA-induced adipogenic differentiation of bovine skeletal muscle satellite cells. In-depth exploration of the molecular mechanism of OA-induced adipogenic differentiation of bovine skeletal muscle satellite cells and related metabolic pathways lay the foundation.

Key words： transcriptome sequencing; oleic acid; Yanbian bovine skeletal muscle satellite cells; adipogenic differentiation

Cite this article

SUN Jianfu , SUN Bin , ZHANG Junfang , WANG Ying , CUI Yan , LI Qiang , CHOI Seong , SHIN Jong , LI Xiangzi . Analysis of Differentially Expressed Genes for Adipogenic Differentiation of Yanbian Bovine Skeletal Muscle Satellite Cells Induced by Oleic Acid Based on Transcriptome Sequencing[J]. Chinese Journal of Animal Nutrition, 2021 , 33(4) : 2263 -2277 . DOI: 10.3969/j.issn.1006-267x.2021.04.045

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