Effects of Buckwheat Straw and Alfalfa Hay Treated by Enzyme and Bacteria on Growth Performance, Slaughter Performance, Rumen Bacterial Diversity and Carbohydrate-Active Enzymes of Tan Sheep

  • JIANG Biwei ,
  • WANG Tian ,
  • ZHOU Yuxiang ,
  • LI Fei
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  • 1. College of Agriculture, Ningxia University, Yinchuan 750021, China;
    2. Ningxia Vocational and Technical College, Yinchuan 750021, China

Received date: 2020-09-02

  Online published: 2021-04-15

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Abstract

This experiment was conducted to study the effects of buckwheat straw and alfalfa hay treated by enzyme and bacteria on growth performance, slaughter performance, rumen bacterial diversity and carbohydrate-active enzymes (CAZy) of Tan sheep. Twenty 3-month-old Ningxia Tan sheep with similar body weight and good health condition were randomly divided into 2 groups with 10 sheep in each group. Control group and trail group had the same concentrate, and the ratio of concentrate to roughage was 30:70, but the control group used untreated buckwheat straw and alfalfa hay (the ratio of straw to alfalfa was 20:80) as roughage, and the trial group used buckwheat straw and alfalfa hay which treated by compound probiotics (main components were yeast, Bacillus subtilis and lactic acid bacteria) and cellulase (activity ≥ 10 000 U/g). The pre feeding period was 15 days and the normal feeding period was 60 days. In the morning of the end of feeding experiment, rumen fluid was collected from fasting oral cavity and DNA was extracted for rumen bacterial diversity and metagenome analysis. After rumen fluid was collected, five sheep in each group with body weight closed to the average body weight were fasted for 24 h, and slaughtered after water deprivation for 2 h. Slaughtering performance and meat quality indexes were measured. The results showed as follows:1) the total gain and average daily gain of Tan sheep in the trial group were significantly higher than those in the control group (P<0.01), and the feed/gain was significantly lower than that in the control group (P<0.01). 2) There were no significant differences in slaughter performance, meat quality indexes and mutton nutrient contents between the trial group and the control group (P>0.05). 3) The Chao index and ACE index in the trial group were significantly lower than those in the control group (P<0.01); at the phylum level, the abundance of Firmicum in the trial group was significantly higher than that in the control group (P<0.05), and the abundances of Bacteroides and Kiritimatiella were significantly lower than those in the control group (P<0.01). At the genus level, the abundances of Prevotella_1 and Kurthia in the trial group was significantly higher than that in the control group (P<0.01). 4) The result of CAZy annotation showed that, in level A, the main families of experimental group and control group were glycoside hydrolase (GH), glycosyltransferase (GT) and carbohydrate binding module (CBM); in level B, the abundances of 12 genes were significantly up-regulated (P<0.05), and the abundances of 3 genes were down regulated (P<0.05). It is concluded that feeding Tan sheep with buckwheat straw and alfalfa hay treated by enzyme and bacteria can improve the growth performance, and tend to improve the meat color and meat quality, at the same time, it is beneficial to the formation of fiber degrading bacteria in the rumen, and affect the microbial diversity of rumen and the gene abundances of some CAZy of Tan sheep. Under the condition of this experiment, the feeding effect of buckwheat straw and alfalfa hay treated by enzyme and bacteria is better, which is beneficial to Tan sheep breeding, can popularize use.

Cite this article

JIANG Biwei , WANG Tian , ZHOU Yuxiang , LI Fei . Effects of Buckwheat Straw and Alfalfa Hay Treated by Enzyme and Bacteria on Growth Performance, Slaughter Performance, Rumen Bacterial Diversity and Carbohydrate-Active Enzymes of Tan Sheep[J]. Chinese Journal of Animal Nutrition, 2021 , 33(4) : 2335 -2346 . DOI: 10.3969/j.issn.1006-267x.2021.04.051

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