Effects of Dietary Threonine in Low Fish Meal Diet on Growth Performance, Body Composition, Amino Acid Composition in Muscle of Triploid Oncorhynchus mykiss

WANG Yaling; WANG Chang'an; LIU Hongbai; LU Shaoxia; ZHANG Yin; XU Zhe

doi:10.3969/j.issn.1006-267x.2021.04.056

2021 , Vol. 33 >Issue 4: 2390 - 2400

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.04.056

Effects of Dietary Threonine in Low Fish Meal Diet on Growth Performance, Body Composition, Amino Acid Composition in Muscle of Triploid Oncorhynchus mykiss

WANG Yaling ,
WANG Chang'an ,
LIU Hongbai ,
LU Shaoxia ,
ZHANG Yin ,
XU Zhe

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1. Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China;
2. College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China;
3. Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214000, China

Received date: 2020-08-14

Online published: 2021-04-15

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Abstract

The purpose of this study was to investigate the effects of dietary threonine in low fish meal diet on growth performance, body composition and amino acid composition in muscle of triploid rainbow trout. The triploid rainbow trout (Oncorhynchus mykiss) with an initial body weight of (18.42±0.20) g was used to the feeding trial for 56 d. Fish reared in a 200 L water aquarium with a water temperature of (15±1)℃. Five kinds of isonitrogenic and isolipidic diets with dietary L-threonine levels of 0.45% (control), 0.76%, 1.09%, 1.29% and 1.64% were prepared based on a basal diet. A total of 450 triploid rainbow trout were randomly divided into 5 groups with 3 replicates per group and 30 replicates per group. The results showed as follows:dietary threonine (0.76% to 1.64%) levels did not significantly affect the feed conversion rate and the protein efficiency compared with the control group (P>0.05). The final body weight,weight gain rate and specific growth rate had significant effects (P<0.05). Based on a quadratic regression analysis between the specific growth rate and protein efficiency, the optimum level was 1.2% to 1.3%(2.89% to 3.13% of dietary crude protein). With the increase of dietary threonine levels, the moisture content of the whole body decreased significantly (P<0.05), and crude lipid contents in 0.75% group was significantly lower than that in the control group (P<0.05). Crude protein contents first increased and then decreased with the increase of threonine level. There were no significant differences in ash contents (P>0.05). The profiles of threonine, methionine, isoleucine, phenylalanine, lysine, aspartic acid, alanine, proline in muscle had no significant difference among the groups (P>0.05), while the contents of valine, leucine, histidine, arginine, serine, glutamic, glycine, cysteine, tyrosine changed significantly, especially those in 1.29% group were significantly higher compared with the control (P<0.05). Therefore, the supplementation of threonine in low fish meal diet can significantly enhance body protein content of triploid rainbow trout. In conclusion, the addition of threonine in low fish meal feed (15%) has the significant growth-promoting effect on triploid rainbow trout, and the optimum requirement is 1.2% to 1.3%.

Key words： threonine; triploid Oncorhynchus mykiss; requirement

Cite this article

WANG Yaling , WANG Chang'an , LIU Hongbai , LU Shaoxia , ZHANG Yin , XU Zhe . Effects of Dietary Threonine in Low Fish Meal Diet on Growth Performance, Body Composition, Amino Acid Composition in Muscle of Triploid Oncorhynchus mykiss[J]. Chinese Journal of Animal Nutrition, 2021 , 33(4) : 2390 -2400 . DOI: 10.3969/j.issn.1006-267x.2021.04.056

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