Correlation Analysis between Relative Abundance of Butyrate-Producing Bacteria and Butyrate Metabolism in Intestine of Jinhua and Landrace Pigs

  • ZHAO Guangmin ,
  • LIU Xiuting ,
  • DAI Bing ,
  • YANG Hua ,
  • LYU Wentao ,
  • WANG Yuanxia ,
  • XIAO Yingping
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  • 1. College of Animal Science, Zhejiang A&F University, Hangzhou 311300, China;
    2. State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China;
    3. Institute of Agro-Product Safety and Nutrition, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received date: 2020-10-17

  Online published: 2021-05-14

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Abstract

The objective of this study was to investigate the relative abundance of butyrate-producing bacteria and its correlation with butyrate metabolism in the intestine of Jinhua and Landrace pigs. Ten Jinhua pigs and 10 Landrace pigs at 240 days of age raised under the same conditions were selected, and the contents of different intestinal segments were collected respectively. The relative abundance of butyrate-producing bacteria in different intestinal segments were revealed by high throughput sequencing, quantitative PCR was performed to determine the relative expression levels of butyryl-CoA:acetyl-CoA transferase and butyrate kinase genes. The content of butyrate was measured by gas chromatography. The results showed as follows:1) the relative abundance of Blautia, Butyricicoccus, Butyricimonas, Faecalibacterium and [Eubacterium] hallii group in jejunum and ileum of Jinhua pigs were significantly lower than those of Landrace pigs (P<0.05). However, the relative abundance of Oscillospira and Roseburia in Jinhua pigs was significantly higher than that of Landrace pigs (P<0.05). There were significant positive correlations among the relative abundance of Blautia, Butyricicoccus, Faecalibacterium and [Eubacterium] hallii group in Jinhua pigs and Landrace pigs (r=0.48 to 0.88,P<0.01). 2) The relative expression levels of butyl-CoA:acetyl-CoA transferase and butyrate kinase genes in the ileum and colon of Jinhua pigs were significantly lower than those of Landrace pigs (P<0.05). 3) The contents of butyrate in jejunum, ileum and colon of Jinhua pigs were significantly lower than those of Landrace pigs (P<0.05). 4) The relative expression levels of butyl-CoA:acetyl-CoA transferase and butyrate kinase genes were positively correlated with the content of butyrate (r=0.570 4 to 0.722 1,P<0.01), and the correlation coefficient of Landrace pigs was higher than that of Jinhua pigs. It is concluded that the relative abundance of butyrate-producing bacteria in jejunum and ileum of Jinhua pigs are significantly lower than those of Landrace pigs. Meanwhile, the relative expression levels of butyrate-producing key functional genes of butyrate kinase and butyl-CoA:acetyl-CoA transferase in colon of Jinhua pigs are significantly lower than those of Landrace pigs. Correspondingly, the content of butyrate in jejunum, ileum and colon of Jinhua pigs is significantly lower than that of Landrace pigs. This also explains to some extent that the intestinal butyrate content of Landrace pigs is higher than Jinhua pigs.

Cite this article

ZHAO Guangmin , LIU Xiuting , DAI Bing , YANG Hua , LYU Wentao , WANG Yuanxia , XIAO Yingping . Correlation Analysis between Relative Abundance of Butyrate-Producing Bacteria and Butyrate Metabolism in Intestine of Jinhua and Landrace Pigs[J]. Chinese Journal of Animal Nutrition, 2021 , 33(5) : 2595 -2604 . DOI: 10.3969/j.issn.1006-267x.2021.05.020

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