Effects of Folic Acid and Vitamin B12 on Performance, Small Intestinal Barrier Function and Related Gene Expression of Laying Hens

WU Xiaotian; WEN Zhao; LI Ruirui; BAI Yan; WANG Rui; YANG Yu

doi:10.3969/j.issn.1006-267x.2021.05.022

2021 , Vol. 33 >Issue 5: 2616 - 2630

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.05.022

Effects of Folic Acid and Vitamin B₁₂ on Performance, Small Intestinal Barrier Function and Related Gene Expression of Laying Hens

WU Xiaotian ,
WEN Zhao ,
LI Ruirui ,
BAI Yan ,
WANG Rui ,
YANG Yu

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College of Animal Science, Shanxi Agricultural University, Taigu 030801, China

Received date: 2020-12-30

Online published: 2021-05-14

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Abstract

The aim of this study was to investigate the effects of dietary different levels of folic acid and vitamin B₁₂ on performance, egg quality, small intestinal tissue morphology, small intestinal barrier function and related gene expression of Jinghong laying hens at peak production. A total of 972 Jinghong laying hens at 28 weeks of age were randomly divided into 9 groups with 6 replicates per group and 18 hens per replicate. Using a two factors with three levels experimental design, the experimental diets were supplemented with folic acid and vitamin B₁₂ at different doses in the basal diet, the folic acid was set at three supplemental levels of 0, 2 and 5 mg/kg, and vitamin B₁₂ was set at three supplemental levels of 0, 25 and 75 μg/kg, respectively. The adjustment period lasted for 2 weeks, and the experimental period lasted for 8 weeks. The results showed as follows:1) dietary folic acid and vitamin B₁₂ and their interaction had no significant effects on the performance of Jinghong laying hens (P>0.05). 2) Dietary 2 mg/kg folic acid significantly increased the egg shape index, egg shell thickness, yolk color and Haugh unit (P<0.05), dietary 25 μg/kg vitamin B₁₂ significantly increased the egg shell thickness and Haugh unit (P<0.05), and their interaction significantly increased the Haugh unit (P<0.05). 3) Dietary 2 mg/kg folic acid significantly increased the villus height and villus height to crypt depth ratio (V/C) in jejunum and ileum (P<0.05), dietary 25 μg/kg vitamin B₁₂ significantly increased the villus height and V/C in duodenum (P<0.05). The interaction of folic acid and vitamin B₁₂ had a significant effect on the villus height and V/C in duodenum and jejunum (P<0.05), which in the 2 mg/kg folic acid and 25 μg/kg vitamin B₁₂ supplemental group were significantly higher than those in the untreated group (P<0.05). 4) Dietary folic acid and vitamin B₁₂ and their interaction had significant effects on the mRNA relative expression levels of occludin and mucins 2 (MUC2) in duodenum and jejunum (P<0.05), which in the 2 mg/kg folic acid and 25 μg/kg vitamin B₁₂ supplemental group were significantly higher than those in the untreated group (P<0.05). 5) Dietary 2 mg/kg folic acid significantly decreased the mRNA relative expression levels of Toll-like receptor 4 (TLR4), nuclear factor-κB (NF-κB), tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) (P<0.05), and significantly increased the interleukin-13 (IL-13) mRNA relative expression level (P<0.05) in duodenum, jejunum and ileum; dietary 25 μg/kg vitamin B₁₂ significantly decreased the mRNA relative expression levels of TLR4 and TNF-α (P<0.05), and significantly increased the interleukin-10 (IL-10) mRNA expression level in duodenum, jejunum and ileum (P<0.05). The interaction of folic acid and vitamin B₁₂ had a significant effect on the mRNA relative expression levels of TLR4, NF-κB, TNF-α, IL-1β and IL-10 in duodenum and jejunum (P<0.05). The mRNA relative expression levels of TLR4, NF-κB, TNF-α and IL-1β in duodenum and jejunum in the 2 mg/kg folic acid and 25 μg/kg vitamin B₁₂ supplemental group were significantly lower than those in the untreated group (P<0.05), and the IL-10 mRNA relative expression level in duodenum and jejunum was significantly higher than that in the untreated group (P<0.05). In conclusion, appropriate level of dietary folic acid and vitamin B₁₂ can improve the egg Haugh unit, small intestinal tissue morphology, small intestinal barrier function and expression of genes related to intestinal inflammation of Jinghong laying hens at peak production. It is better to add 2 mg/kg folic acid and 25 μg/kg vitamin B₁₂ to the diet (containing 1.35 mg/kg folic acid and 25 μg/kg vitamin B₁₂).

Key words： folic acid; vitamin B₁₂; laying hens; performance; intestinal morphology; intestinal barrier function

Cite this article

WU Xiaotian , WEN Zhao , LI Ruirui , BAI Yan , WANG Rui , YANG Yu . Effects of Folic Acid and Vitamin B₁₂ on Performance, Small Intestinal Barrier Function and Related Gene Expression of Laying Hens[J]. Chinese Journal of Animal Nutrition, 2021 , 33(5) : 2616 -2630 . DOI: 10.3969/j.issn.1006-267x.2021.05.022

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