Effects of Adding Protease on Metabolizable Energy Values of Rapeseed Meal in Meat Ducks

  • YU Shuang ,
  • WANG Jianping ,
  • ZENG Qiufeng ,
  • DING Xuemei ,
  • BAI Shiping ,
  • ZHANG Keying
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  • Key Laboratory for Animal Disease Resistance Nutrition of the Ministry of Education, Key Laboratory of Animal Disease Resistance nutrition of Ministry of Agriculture and Rural Affairs, Key Laboratory of Animal Disease Resistance Nutrition of Sichuan Province, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu 611130, China

Received date: 2020-11-06

  Online published: 2021-05-14

Supported by

 

Abstract

The purpose of this experiment was to evaluate the improvement effect of adding protease on the metabolizable energy values of rapeseed meal and the nutrient true availabilities of diets for meat ducks. Healthy cherry-grain male ducks over 19 weeks of age were selected for metabolism test by true metabolizable energy (TME) method combined with set algorithm, and 5 rapeseed meals with different protein solubilities were selected (named A1, A2, A3, A4 and A5, respectively). Rapeseed meal was used to replace corn at 40%, and soybean meal was used to replace corn at 35%. Thirteen groups were set, including 1 corn group, 1 soybean meal group, 1 hungry endogenous group, 5 rapeseed meal groups without protease addition, and 5 rapeseed meal groups with protease addition (the addition level of protease preparation was 200 mg/kg, and the protease activity in the protease preparation was 1×105 U/g). Each group had 12 replicates with 1 duck per replicate. The results showed as follows:1) the true availabilities of dry matter (DM), ether extract (EE), crude protein (CP), neutral detergent fiber (NDF), acid detergent fiber (ADF), crude ash (Ash), calcium (Ca) and phosphorus (P) in rapeseed meal diets were increased by 3.73% (P<0.05), 9.92% (P<0.05), 8.51% (P<0.05), 15.78% (P<0.05), 17.09% (P<0.05), 2.12% (P>0.05), 8.55% (P<0.05), 2.81% (P>0.05) and 12.96% (P<0.05) by adding protease, respectively. 2) Adding protease could significantly improve the apparent metabolizable energy (AME), TME, nitrogen correction apparent metabolizable energy (AMEn) and nitrogen correction true metabolizable energy (TMEn) of rapeseed meal (P<0.05), and the improvement degrees of them were 2.01, 2.01, 1.80 and 1.80 MJ/kg, respectively. It can be concluded that adding protease can improve the metabolizable energy values of rapeseed meal and the nutrient true availabilities of rapeseed meal diet.

Cite this article

YU Shuang , WANG Jianping , ZENG Qiufeng , DING Xuemei , BAI Shiping , ZHANG Keying . Effects of Adding Protease on Metabolizable Energy Values of Rapeseed Meal in Meat Ducks[J]. Chinese Journal of Animal Nutrition, 2021 , 33(5) : 2672 -2680 . DOI: 10.3969/j.issn.1006-267x.2021.05.027

References

[1] 张妮娅.添加酶制剂对改善"双低"菜籽饼粕营养物质及能量代谢的研究[D].硕士学位论文.武汉:华中农业大学,2003. ZHANG N Y.Study on improving nutrient and energy metabolism of double low rapeseed cake by adding enzyme preparation[D].Master's Thesis.Wuhan:Huazhong Agricultural University,2003.(in Chinese)
[2] GHAZI S,ROOKE J A,GALBRAITH H,et al.The potential for the improvement of the nutritive value of soya-bean meal by different proteases in broiler chicks and broiler cockerels[J].British Poultry Science,2002,43(1):70-77.  
[3] 汪银锋,李素平,张莹蕾,等.饲用酶制剂在饲料中的应用[J].饲料博览(技术版),2008(5):27-30. WANG Y F,LI S P,ZHANG Y L,et al.Application of forage enzyme preparation in feed[J].Feed Review (Technical Version),2008(5):27-30.(in Chinese)
[4] ANGEL C R,SAYLO R W,VIEIRA S L,et al.Effects of a monocomponent protease on performance and protein utilization in 7- to 22-day-old broiler chickens[J].Poultry Science,2011,90(10):2281-2286.  
[5] RUBIO L A,BRENES A,CENTENO C.Effects of feeding growing broiler chickens with practical diets containing sweet lupin (Lupinus angustifolius) seed meal[J].British Poultry Science,2003,44(3):391-397.  
[6] BAREKATAIN M R,ANTIPATIS C,CHOCT M,et al.Interaction between protease and xylanase in broiler chicken diets containing sorghum distillers' dried grains with solubles[J].Animal Feed Science and Technology,2013,182(1/2/3/4):71-81.
[7] LIU S Y,SELLE P H,COURT S G,et al.Protease supplementation of sorghum-based broiler diets enhances amino acid digestibility coefficients in four small intestinal sites and accelerates their rates of digestion[J].Animal Feed Science and Technology,2013,183(3/4):175-183.
[8] 张开心,张扬,张克英,等.蛋白酶对肉鸭菜籽粕标准回肠氨基酸消化率的影响[J].动物营养学报,2019,31(7):3285-3293. ZHANG K X,ZHANG Y,ZHANG K Y,et al.Effects of protease on standard ileum amino acid digestibility of rapeseed meal in meat ducks[J].Chinese Journal of Animal Nutrition,2019,31(7):3285-3293.(in Chinese)
[9] ADEOLA O,NYACHOTI C M,RAGLAND D.Energy and nutrient utilization responses of ducks to enzyme supplementation of soybean meal and wheat[J].Canadian Journal of Animal Science,2007,87(2):199-205.  
[10] 李东东,李宗锐,丁雪梅,等.不同粗蛋白质水平饲粮添加外源蛋白酶对肉鸡生产性能、血清生化指标和抗氧化功能的影响[J].动物营养学报,2015,27(9):2820-2831. LI D D,LI Z R,DING X M,et al.Effects of different crude protein levels diet supplemented with exogenous protease on performance,serum biochemical indices and antioxidant function of broilers[J].Chinese Journal of Animal Nutrition,2015,27(9):2820-2831.(in Chinese)
[11] FREITAS D M,VIEIRA S L,ANGEL C R,et al.Performance and nutrient utilization of broilers fed diets supplemented with a novel mono-component protease[J].Journal of Applied Poultry Research,2011,20(3):322-334.  
[12] YANG Z B,YANG W R,JIANG S Z,et al.Effects of a thermotolerant multi-enzyme product on nutrient and energy utilization of broilers fed mash or crumbled corn-soybean meal diets[J].Journal of Applied Poultry Research,2010,19(1):38-45.  
[13] 郭正富,杨小琴,李军.高粱替代玉米日粮添加蛋白酶对肉鸡生长性能、养分利用率和肉质的影响[J].中国饲料,2018(4):23-28. GUO Z F,YANG X Q,LI J.Effects of sorghum substitutes corn diet with proteases supplementation on growth performance,nutrient utilization and meat quality of broilers[J].China Feed,2018(4):23-28.(in Chinese)
[14] 李霞.溢多杂粕酶在肉鸭杂粕饲粮中的应用效果及作用机理研究[D].硕士学位论文.重庆:西南大学,2008. LI X.Study on application and mechanism of Yiduo enzyme preparation in corn-rapeseed meal diet of duck[D].Master's Thesis.Chongqing:Southwest University,2008.(in Chinese)
[15] 奚刚,许梓荣.中性蛋白酶制剂对丝毛乌骨鸡氮利用率及内源性蛋白质消化酶活性的影响[J].浙江大学学报(农业与生命科学版),1998,24(4):399-404. XI G,XU Z R.Effect of neutral protease preparation on nitrogen utilization rate and endogenous protein-digestive enzyme activity of silky black bone chicken[J].Journal of Zhejiang University (Agriculture and Life Sciences Edition),1998,24(4):399-404.(in Chinese)
[16] 胡秀华.不同酶制剂对肉鸡饲粮养分回肠消化率的影响及作用机理研究[D].硕士学位论文.雅安:四川农业大学,2006. HU X H.The effects of different enzyme preparation on the ileal nutrient digestibility and the mechanisms in broilers[D].Master's Thesis.Ya'an:Sichuan Agricultural University,2006.(in Chinese)
[17] DOUGLAS M W,PARSONS C M,BEDFORD M R.Effect of various soybean meal sources and avizyme on chick growth performance and ileal digestible energy[J].Journal of Applied Poultry Research,2000,9(1):74-80.  
[18] GHAZI S,ROOKE J A,GALBRAITH H.Improvement of the nutritive value of soybean meal by protease and α-galactosidase treatment in broiler cockerels and broiler chicks[J].British Poultry Science,2003,44(3):410-418.  
[19] 唐德富,郝生燕,汝应俊,等.不同来源菜籽粕有效能值的变异度分析及酶制剂的添加效果研究[J].中国饲料,2014(17):27-30,36. TANG D F,HAO S Y,RU Y J,et al.Study on available energy variability from different rapeseed meal and feeding effects of enzyme supplementation[J].China Feed,2014(17):27-30,36.(in Chinese)
[20] 刘干,姜宁,张爱忠.蛋白酶的生物学意义及在肉鸡日粮中的应用研究进展[J].饲料博览,2018(11):11-12,16. LIU G,JIANG N,ZHANG A Z.Application and research progress of protease in broiler diet[J].Feed Review,2018(11):11-12,16.(in Chinese)
[21] ANDERSON-HAFERMANN J C,ZHANG Y C,PARSONS C M.Effects of processing on the nutritional quality of canola meal[J].Poultry Science,1993,72(2):326-333.  
[22] PASTUSZEWSKA B,JABŁECKI G,BURACZEWSKA L,et al.The protein value of differently processed rapeseed solvent meal and cake assessed by in vitro methods and in tests with rats[J].Animal Feed Science and Technology,2003,106(1/2/3/4):175-188.
[23] 唐精,张宝彤,李爱琴,等.蛋白溶解度在四种饼粕品质评价中的应用[J].广东饲料,2013,22(7):32-34. TANG J,ZHANG B T,LI A Q,et al.Application of protein solubility in quality evaluation of four kinds of cake[J].Guangdong Feed,2013,22(7):32-34.(in Chinese)
[24] 初雷,李爱科,高玉鹏,等.菜籽粕品质指标变化规律及相互关系研究[J].中国油脂,2009,34(11):20-23. CHU L,LI A K,GAO Y P,et al.Changes and relationships of the quality indexes of rapeseed meal[J].China Oils and Fats,2009,34(11):20-23.(in Chinese)
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