Effects of Different Probiotics on Growth Performance, Serum Biochemical Indexes and Fecal Microflora of Weaned Goats

LU Jiawei; WANG Jingang; GAO Qin; LIU Zifei; CHEN Zili; HUANG Qunhao; ZHANG Yanli; WANG Feng

doi:10.3969/j.issn.1006-267x.2021.05.034

Chinese Journal of Animal Nutrition >

2021 , Vol. 33 >Issue 5: 2752 - 2764

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.05.034

Effects of Different Probiotics on Growth Performance, Serum Biochemical Indexes and Fecal Microflora of Weaned Goats

LU Jiawei ,
WANG Jingang ,
GAO Qin ,
LIU Zifei ,
CHEN Zili ,
HUANG Qunhao ,
ZHANG Yanli ,
WANG Feng

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1. College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China;
2. Research of Haimen Goats, Nanjing Agricultural University, Haimen 216121, China;
3. Animal Husbandry and Veterinary Station of Haimen District, Nantong City, Nantong 226100, China

Received date: 2020-11-03

Online published: 2021-05-14

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Abstract

This experiment aimed at exploring the effects of Bacillus subtilis and Bacillus licheniformis feeding alone and combined on the growth performance, diarrhea rate, serum biochemical indexes and fecal microflora of weaned goats. Sixty 3-month-old Haimen goats were selected and randomly divided into 4 groups with 3 replicates in each group and 5 goats in each replicate. Control group (C group) was fed a basal diet; Bacillus subtilis group (BS group) was fed the basal diet + 400 mg/kg of Bacillus subtilis (effective bacteria number was 1×10¹¹ CFU/g); Bacillus licheniformis group (BL group) was fed the basal diet + 400 mg/kg of Bacillus licheniformis (effective bacteria count was 1×10¹¹ CFU/g); BS_BL group (combined group) was fed the basic diet + 400 mg/kg of Bacillus subtilis+ 400 mg/kg of Bacillus licheniformis (effective bacteria number was 1×10¹¹ CFU/g). The pre-feeding period lasted for 10 d and the experimental period lasted for 30 d. The results showed as follows:1) on day 10 of the experiment, the weight of weaned goats in the BS_BL group was significantly higher than that in the C, BS and BL groups (P<0.01); on day 30, there was no significant difference in the weight of weaned goats among the C, BS, BL and BS_BL groups (P>0.05); on days 1 to 30, the average daily gain (ADG) in the BS_BL group was significantly higher than that in the C, BS and BL groups (P<0.01); on days 21 to 30 of the experiment, the ADG in the BL and the BS_BL groups was significantly higher than that in the C and BS groups (P<0.01); on days 1 to 30, there were no significant difference in ADG between experimental groups and C group (P>0.05); the average daily feed intake (ADFI) in the BS_BL group was significantly higher than that in the C, BS and BL groups on days 21 to 30 of the experiment (P<0.01); the ADFI in the BS_BL group was significantly higher than that in the C, BS and BL groups on days 1 to 30 of the experiment (P<0.05); on days 1 to 30 of the experiment, the feed to gain ratio (F/G) had no significant difference among groups (P>0.05). 2) Compared with C group, the diarrhea rate in BS, BL and BS_BL groups was significantly reduced on days 1 to 10 and days 11 to 20 of the experiment (P<0.05), and the diarrhea rate in BS_BL group was significantly reduced on days 1 to 30 of the experiment (P<0.05). 3) Compared with C group, on day 30 of the experiment, the activities of alkaline phosphatase (ALP) and lactate dehydrogenase (LDH) and the content of glucose (GLU) in serum in the BS group were significantly reduced (P<0.05), the activity of aspartate aminotransferase (AST) and the content of albumin (ALB) in serum were extremely significantly reduced (P<0.01), the content of urea nitrogen (UN) in serum in the BL group was significantly increased (P<0.05), and the activity of AST and the content of GLU in serum in the BS_BL group were significantly reduced (P<0.05). 4) The 16S rDNA sequencing of feces found that Chao 1 and observed-species indexes showed that the BS_BL group contained more species, and the Shannon and Simpson indexes showed that the BS_BL group had higher diversity. The dominant phyla of bacteria in C group were Proteobacteria, Bacteroidetes and Actinobacteria; the dominant phyla of bacteria in BS group were Actinobacteria, Proteobacteria and Bacteroidetes; the dominant phyla of bacteria in BL group were Bacteroidetes, Proteobacteria and Planctomycetes; the dominant phyla of bacteria in BS_BL group were Bacteroidetes, Proteobacteria and Firmicutes. The dominant genus of bacteria in C group were Sorangium, Pedobacter and Sphaerimonospora; the dominant genus of bacteria in BS group were Nonomuraea, Altererythrobacte and Sorangium; the dominant genus in the BL group belonged to Parapedobacter, Nonomuraea and Planctomicrobium; the dominant genus of bacteria in BS_BL group were Marinelabiliaceae_unclassified, Pseudomonas, Clostridiales_vadinBB60_group_unclassified. In summary, the combined feeding of Bacillus subtilis and Bacillus licheniformis can significantly improve the growth performance of weaned goats, reduce the rate of diarrhea, improve serum biochemical indexes, and change the diversity and abundance of fecal microflora.

Key words： probiotics; weaned goats; growth performance; serum biochemical indexes; fecal microflora

Cite this article

LU Jiawei , WANG Jingang , GAO Qin , LIU Zifei , CHEN Zili , HUANG Qunhao , ZHANG Yanli , WANG Feng . Effects of Different Probiotics on Growth Performance, Serum Biochemical Indexes and Fecal Microflora of Weaned Goats[J]. Chinese Journal of Animal Nutrition, 2021 , 33(5) : 2752 -2764 . DOI: 10.3969/j.issn.1006-267x.2021.05.034

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