Effects of Dietary Choline Chloride Content on Rumen Microbiota and Plasma Trimethylamine N-Oxide Content in Hu Sheep

  • ZHOU Yang ,
  • MEI Shijun ,
  • MAO Shengyong ,
  • JIN Wei
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  • 1. National Joint Research Center for Animal Digestive Tract Nutrition, Jiangsu Province Key Laboratory of Gastrointestinal Nutrition and Animal Health, Laboratory of Gastrointestinal Microbiology, Nanjing Agricultural University, Nanjing 210095, China;
    2. National Experimental Teaching Center for Animal Science, Nanjing Agricultural University, Nanjing 210095, China

Received date: 2020-10-22

  Online published: 2021-05-14

Supported by

 

Abstract

The aim of this study was to investigate the response of rumen microbiota and plasma trimethylamine N-oxide (TMAO) content to the change of dietary choline chloride content in Hu sheep. Twelve healthy male Hu-sheep with similar body weight in fattening period were randomly arranged into control and experimantal groups, 6 in each group. In the control group, sheep were fed a basal diet. In experimental group, sheep were supplemented with 0.05% choline chloride based on the basal diet for 28 days. Blood samples and rumen contents were collected before morning feeding at days 27 and 28, respectively. The results showed as follows:there was no significant difference in growth performance and rumen fermentation parameters between experimental group and control group (P>0.05). The plasma TMAO content and rumen microbial populations were analyzed using targeted metabonomics, high-throughput sequencing and real-time PCR technologies. Results showed that TMAO content in plasma tended to increase in experimental group (P=0.075). PCoA analysis showed that the choline chloride changed the bacterial community structure. LDA analysis showed that Veillonellaceae and Oribacterium enriched in control group, Bacteroidaceae, Bacteroides, Mollicutes, Tenericutes, Gammaproteobacteria and RF9 enriched in experimantal group. Real-time PCR results showed that choline chloride promoted Methanomassiliicoccales proliferation (P=0.016), but did not affect the choline-degrading bacterial cutC gene abundance (P=0.462). The addition of 0.05% choline chloride change the community structure of rumen bacteria and methanogens in Hu sheep, and has a tendency to increase the TMAO content in plasma.

Cite this article

ZHOU Yang , MEI Shijun , MAO Shengyong , JIN Wei . Effects of Dietary Choline Chloride Content on Rumen Microbiota and Plasma Trimethylamine N-Oxide Content in Hu Sheep[J]. Chinese Journal of Animal Nutrition, 2021 , 33(5) : 2786 -2794 . DOI: 10.3969/j.issn.1006-267x.2021.05.037

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