Effects of Dietary Bile Acid Level on Growth Performance, Nitrogen Metabolism and Serum Biochemical Indexes of Growing Raccoon Dogs

  • PAN Sumin ,
  • SHEN Shurui ,
  • ZHANG Haihua ,
  • LIU Xin ,
  • KONG Lingyu ,
  • WEI Xue
Expand
  • Hebei Key Laboratory of Specialty Animal Germplasm Resources Exploration and Innovation, College of Animal Science and Technology, Hebei Normal University of Science and Technology, Qinhuangdao 066000, China

Received date: 2020-10-28

  Online published: 2021-05-14

Supported by

 

Abstract

The purpose of this study was to investigate the effects of dietary bile acid level on the growth performance, nutrient apparent digestibilites, nitrogen metabolism and serum biochemical indexes of raccoon dogs during the growing period. A total of 64 male raccoon dogs aged 50 days were selected and randomly divided into 4 groups with 16 repetitions in each group and 1 raccoon dog in each repetition. Raccoon dogs in the 4 groups were fed a basal diet supplemented with bile acid levels of 0 (group Ⅰ), 100 (group Ⅱ), 300 (group Ⅲ), 500 mg/kg bile acids (group Ⅳ), respectively. The trial lasted for 90 days. The results showed as follows:1) the final weight of group Ⅱ was significantly higher than that of group Ⅰ (P<0.05). The material-to-weight ratio of group Ⅱ was significantly lower than that of group Ⅰ (P<0.05). 2) The dry matter apparent digestibility of group Ⅱ was significantly higher than that of group Ⅳ (P<0.05). The crude protein apparent digestibility of group Ⅰ was significantly higher than that of group Ⅳ (P<0.05), and the crude protein digestibility of group Ⅱ was significantly higher than that of group Ⅳ (P<0.01). The ether extract apparent digestibility of group Ⅰ was significantly lower than that of group Ⅱ, group Ⅲ and group Ⅳ (P<0.05). 3) Fecal nitrogen output of group Ⅱ and group Ⅰ was significantly lower than that of group Ⅳ (P<0.05). The nitrogen deposition of group Ⅱ was significantly higher than that of group Ⅳ (P<0.05). 4) The serum urea content of group Ⅳ was significantly higher than that of group Ⅰ (P<0.01), group Ⅲ was significantly higher than group Ⅰ (P<0.05), and group Ⅳ was significantly higher than group Ⅱ (P<0.05). The serum total protein and albumin contents of group Ⅱ, group Ⅲ and group Ⅳ were significantly higher than those of group Ⅰ (P<0.01). The serum alanine transaminase activity of group Ⅳ was significantly higher than that of group Ⅰ (P<0.01), and that of group Ⅳ was significantly higher than that of group Ⅱ and group Ⅲ (P<0.05). Serum alkaline phosphatase activity of group Ⅲ and group Ⅳ was significantly higher than that of group Ⅰ (P<0.01), and group Ⅳ was significantly higher than group Ⅱ (P<0.05). The serum glucose content of group Ⅳ was significantly higher than that of group Ⅰ (P<0.01). The serum high-density lipoprotein content of group Ⅲ and group Ⅳ was significantly higher than that of group Ⅰ (P<0.01), group Ⅱ was significantly higher than group Ⅰ (P<0.05), and group Ⅳ was significantly higher than group Ⅱ (P<0.05). The serum low density lipoprotein content of group Ⅳ was significantly higher than that of group Ⅰ (P<0.01), significantly higher than that of group Ⅱ (P<0.05). The serum cholesterol content of group Ⅲ and group Ⅳ was significantly higher than that of group Ⅰ (P<0.01), group Ⅱ was significantly higher than group Ⅰ (P<0.05), and group Ⅳ was significantly higher than group Ⅱ (P<0.05). Serum triglyceride content of group Ⅳ was significantly higher than that of group Ⅰ and group Ⅱ (P<0.01). Under the conditions of this experiment, the growth performance, dry matter apparent digestibility, crude protein apparent digestibility, ether extract apparent digestibility and nitrogen deposition of raccoon dogs are higher when the dietary bile acid addition level is 100 mg/kg. At this level, the animal body has better absorption of protein and fat, which is more beneficial to the growth of raccoon dogs.

Cite this article

PAN Sumin , SHEN Shurui , ZHANG Haihua , LIU Xin , KONG Lingyu , WEI Xue . Effects of Dietary Bile Acid Level on Growth Performance, Nitrogen Metabolism and Serum Biochemical Indexes of Growing Raccoon Dogs[J]. Chinese Journal of Animal Nutrition, 2021 , 33(5) : 2887 -2895 . DOI: 10.3969/j.issn.1006-267x.2021.05.046

References

[1] 王进,孙磊,韩凤福.胆汁酸对生猪生产性能的影响[J].畜牧兽医科技信息,2018(1):18-19. WANG J,SUN L,HAN F F.Effect of bile acid on performance of pig[J].Animal Husbandry and Veterinary Science and Technology Information,2018(1):18-19.(in Chinese)
[2] 程伟伟,王少琨,王进圣,等.日粮添加胆汁酸对肉鸡生产性能、血清生化指标与屠宰性能的影响[J].中国家禽,2017,39(3):31-35. CHENG W W,WANG S K,WANG J S,et al.Effects of dietary bile acids on production performance,serum biochemical index and slaughter performance in broilers[J].China Poultry,2017,39(3):31-35.(in Chinese)
[3] 杨玉芝,刘敬盛,李燕舞,等.胆汁酸对"817"肉杂鸡生产性能的影响[J].中国家禽,2009,31(12):62-63,65. YANG Y Z,LIU J S,LI Y W,et al.Effect of bile acids on performance of "817" broilers[J].China Poultry,2009,31(12):62-63,65.(in Chinese)
[4] 曾本和,向枭,周兴华,等.高脂饲料中胆汁酸水平对齐口裂腹鱼肠道组织结构及脂肪代谢酶活性的影响[J].水产学报,2016,40(9):1340-1348. ZENG B H,XIANG X,ZHOU X H,et al.Effects of bile acid level in high lipid diet on intestinal structure and lipid metabolism enzyme activity of juvenile Schizothorax prenanti[J].Journal of Fisheries of China,2016,40(9):1340-1348.(in Chinese)
[5] 李漠.育成期和冬毛期貉饲粮蛋白和脂肪添加水平的研究[D].硕士学位论文.秦皇岛:河北科技师范学院,2018. LI M.Optimal levels of dietary protein and fat supplement for growing and fur development raccoon dogs[D].Master's Thesis.Qinhuangdao:Hebei Normal University of Science and Technology,2018.(in Chinese)
[6] 巩文洋,李默,牛一兵,等.日粮粗蛋白质和粗脂肪水平对生长貉营养物质消化率和氮代谢的影响[J].中国畜牧兽医,2020,47(3):763-770. GONG W Y,LI M,NIU Y B,et al.Effects of dietary protein and ether extract levels on nutrient digestibility and nitrogen metabolism of growing raccoon dogs[J].China Animal Husbandry & Veterinary Medicine,2020,47(3):763-770.(in Chinese)
[7] 王亚琪.复合饲料添加剂在乌苏里貉生产中的应用效果研究[D].硕士学位论文.秦皇岛:河北科技师范学院,2019. WANG Y Q.Study on the application effect of compound feed additive in the production of raccoon dog[D].Master's Thesis.Qinhuangdao:Hebei Normal University of Science and Technology,2019.(in Chinese)
[8] 杨家昶.应激对肉鸡胆汁酸代谢影响机制的研究[D].硕士学位论文.泰安:山东农业大学,2016. YANG J C.Research of effects of stress on poultry bile acid metabolism pathway[D].Master's Thesis.Tai'an:Shandong Agricultural University,2016.(in Chinese)
[9] ZHANG Y Q,LEE F Y,BARRERA G,et al.Activation of the nuclear receptor FXR improves hyperglycemia and hyperlipidemia in diabetic mice[J].Proceedings of the National Academy of Sciences of the United States of America,2006,103(4):1006-1011.  
[10] 左旭冬.鸡蛋内胆汁酸沉积的调控及其对子代生长与肌纤维发育的影响[D].硕士学位论文.广州:华南农业大学,2016. ZUO X D.Regulation of bile acid deposition in eggs and the effect on growth performance and muscle fiber development in offspring[D].Master's Thesis.Guangzhou:South China Agricultural University,2016.(in Chinese)
[11] 鲁慧文,郭若婷,陈红兴,等.杜洛克公猪不同阶段料重比的相关性研究[J].猪业科学,2019,36(8):102-104. LU H W,GUO R T,CHENG H X,et al.Study on feed specific gravity of Duroc boar at different stages[J].Pig Science,2019,36(8):102-104.(in Chinese)
[12] 赵盼月.饲料中添加胆汁酸对欧洲鳗鲡幼鱼生长、血清生化指标、肠道菌群及肝脏代谢的影响[D].硕士学位论文.厦门:集美大学,2019. ZHAO P Y.Effects of dietary bile acid supplementation on growth performance,serum biochemical parameters,intestinal flora and liver metabonomics of juvenile European eel[D].Master's Thesis.Xiamen:Jimei University,2019.(in Chinese)
[13] 孙建珍.添加胆汁酸对大菱鲆(Scophthalmus maximus)幼鱼脂肪利用能力的影响[D].硕士学位论文.上海:上海海洋大学,2014. SUN J Z.Effects of dietary lipid and bile acid levels on growth,body composition,digestibility physiology and lipid metabolism of juvenile turbot (Scophthalmus maximus)[D].Master's Thesis.Shanghai:Shanghai Ocean University,2014.(in Chinese)
[14] 吴明海.奶牛日粮中添加胆汁酸盐饲喂效果研究[D].硕士学位论文.石河子:石河子大学,2019. WU M H.Study on the effects of bile salt in dairy cows diet[D].Master's Thesis.Shihezi:Shihezi University,2019.(in Chinese)
[15] 蔡元丽,谢幼梅,魏可峰,等.大豆磷脂及其在动物饲料中的应用[J].中国饲料,2002(10):8-10. CAI Y L,XIE Y M,WEI K F,et al.Soybean phospholipid and its application in animal feed[J].Chinese Feed,2002(10):8-10.(in Chinese)
[16] 唐精,王继强,张宝彤,等.胆汁酸的代谢与生理功能[J].海洋与渔业,2011(7):65-66. TANG J,WANG J Q,ZHNAG B T,et al.Metabolism and physiological function of bile acids[J].Ocean and Fishery,2011(7):65-66.(in Chinese)
[17] MONTE M J,MARIN J J G,ANTELO A,et al.Bile acids:chemistry,physiology,and pathophysiology[J].World Journal of Gastroenterology,2009,15(7):804-816.  
[18] BORG B S,LIBAL G W,WAHLSTROM R C.Tryptophan and threonine requirements of young pigs and their effects on serum calcium,phosphorus and zinc concentrations[J].Journal of Animal Science,1987,64(4):1070-1078.  
[19] MALMLOF K.Amino acid in farm animal nutrition metabolism,partition and consequences[J].Swedish Journal of Agricultural Research,1988,18(4):191-193.
[20] 李红军.那西肽对肉仔鸡生长性能的影响及其作用机理的探讨[D].硕士学位论文.杭州:浙江大学,2004. LI H J.Effeet of nosiheptide on growth performance and approach to the mechanism in broilers[D].Master's Thesis.Hangzhou:Zhejiang University,2004.(in Chinese)
[21] 王灿,苗志敏,李长贵,等.人体血尿酸水平对血清谷丙转氨酶和谷草转氨酶水平的影响[J].山东医药,2010,50(29):1-3. WANG C,MIAO Z M,LI C G,et al.Impact of uric acid level on alanine amiotransferase and aspartate aminotransferase[J].Shandong Medicine,2010,50(29):1-3.(in Chinese)
[22] 张玲,张卫东,张小玲,等.氯化胆碱、肌醇和胆汁酸对鲤鱼生长、血清生化及免疫指标的影响[J].饲料工业,2008,29(22):14-16. ZHANG L,ZHANG W D,ZHANG X L,et al.The effects of choline chloride,inositol and bile acid on growth performance,biochemical and immune indexes of carps[J].Feed Industry,2008,29(22):14-16.(in Chinese)
[23] WOOLLETT L A,BUCKLEY D D,YAO L H,et al.Cholic acid supplementation enhances cholesterol absorption in humans[J].Gastroenterology,2004,126(3):724-731.  
[24] 周书耘,刘永坚,梁海鸥,等.饲料中添加胆汁酸对军曹鱼生长及体组成的影响[J].南方水产,2010,6(4):20-25. ZHOU S Y,LIU Y J,LIANG H O,et al.Effect of bile acids on growth and body composition of cobia (Rachycentron canadun)[J].South China Fisheries Science,2010,6(4):20-25.(in Chinese)
[25] 张国安,王继芬,王振业,等.红鱼粉型饲料中添加胆汁酸对大菱鲆生长及肝功能的影响[J].水产科学,2018,37(5):599-604. ZHANG G A,WANG J F,WANG Z Y,et al.Effects of brown fishmeal diet supplemented with bile acid on growth performance and hepatic function in turbot[J].Fisheries Science,2018,37(5):599-604.(in Chinese)
Outlines

/