Digestive Characteristics and Associative Effects of Whole Corn Silage, Wheat Straw and Alfalfa Hay in Vitro

  • CHENG Jing ,
  • ZHANG Yuanqing ,
  • ZHANG Dandan ,
  • LI Bo ,
  • JIN Guang ,
  • WANG Dongcai ,
  • XU Fang ,
  • SUN Ruifeng
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  • College of Animal Science, Shanxi Agricultural University(Shanxi Academy of Agricultural Science), Taiyuan 030032, China

Received date: 2020-10-30

  Online published: 2021-05-14

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Abstract

The aim of this experiment was to select the best roughage combination of fermented total mixed ration by studying the digestive characteristics and associative effects of different proportions of whole corn silage (WCS), wheat straw (WS) and alfalfa hay (AH) in vitro. Ten combined forages were prepared with different proportions of WCS, WS and AH [WCS:WS:AH=80:10:10, 70:10:20, 70:20:10, 60:30:10, 60:20:20, 60:10:30, 50:10:40, 50:20:30, 50:30:20, 50:40:10, respectively]. Ten combined forages plus three single forages, as 13 fermentation substrates, were fermented in vitro. The fermentation parameters were measured, including gas production (GP), in vitro dry matter digestibility (IVDMD), and pH, the concentrations of ammonia nitrogen (NH3-N) and volatile fatty acid (VFA) in fermentation broth, and the multiply factor associative effects index (MFAEI) of 10 combined forages were calculated. The results were showed as follows:1) among the 10 combined forages, the 72 h GP was the highest when WCS:WS:AH=80:10:10, and it was significantly higher than that of other combinations except WCS:WS:AH=70:20:10 (P<0.05). Among the 3 single forages, the 72 h GP of WCS was the highest, which was significantly higher than that of WS and AH (P<0.05). 2) Among the 10 combined forages, the gas production of quickly degraded part (a) and the gas production of slowly degraded part (b) were the highest when WCS:WS:AH=80:10:10, and they were significantly higher than those of other combinations (P<0.05). Among the 3 single forages, WCS has the highest value of a, b and the gas production rate constant of b (c), and they were significantly higher than those of WS (P<0.05). 3) After 72 h fermentation, the IVDMD was the highest when WCS:WS:AH=80:10:10 among the 10 combined forages. Among the 3 single forages, the IVDMD of WCS was the highest. The concentration of NH3-N of AH was significantly higher than that of WS (P<0.05). The pH of AH and WS was significantly higher than that of WCS (P<0.05). 4) The concentrations of acetic acid, propionic acid, butyric acid and total volatile fatty acids (TVFA) in the WCS:WS:AH=70:20:10 combination were significantly higher than those in other combinations (P<0.05). The concentration of TVFA in WCS was significantly higher than that of AH and WS (P<0.05). 5) The MFAEI was the highest (0.613 2) when WCS:WS:AH=70:20:10. 6) The 72 h IVDMD of combined forages was extremely significantly negatively correlated with ash and acid detergent fiber (ADF) contents (P<0.01), and was significantly negatively correlated with neutral detergent fiber (NDF) content (P<0.05). It is concluded that the optimal combined ratio of WCS, WS and AH evaluated by MFAEI is 70:20:10.

Cite this article

CHENG Jing , ZHANG Yuanqing , ZHANG Dandan , LI Bo , JIN Guang , WANG Dongcai , XU Fang , SUN Ruifeng . Digestive Characteristics and Associative Effects of Whole Corn Silage, Wheat Straw and Alfalfa Hay in Vitro[J]. Chinese Journal of Animal Nutrition, 2021 , 33(5) : 2982 -2992 . DOI: 10.3969/j.issn.1006-267x.2021.05.056

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