Effects of Epidermal Growth Factor on Duodenal Morphology, Digestive Enzyme Activities and Epidermal Growth Factor Receptor Expression of Broilers

SHAN Chunqiao; LIU Qiuchen; LI Juan; LIU En; LU Jishuang; LIU Yan

doi:10.3969/j.issn.1006-267x.2021.06.024

2021 , Vol. 33 >Issue 6: 3218 - 3227

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.06.024

POULTRY NUTRITION AND FEED

Effects of Epidermal Growth Factor on Duodenal Morphology, Digestive Enzyme Activities and Epidermal Growth Factor Receptor Expression of Broilers

SHAN Chunqiao ,
LIU Qiuchen ,
LI Juan ,
LIU En ,
LU Jishuang ,
LIU Yan

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1. Dalian Sanyi Bioengineering Research Institute, Dalian 116036, China;
2. Jiangsu Sanyi Scientific Research Quality Control Center, Pizhou 221300, China

Received date: 2020-12-13

Online published: 2021-06-10

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Abstract

The purpose of this experiment was to study the effects of dietary epidermal growth factor (EGF) on duodenal morphology, digestive enzyme activities and epidermal growth factor receptor (EGFR) expression of broilers. Seventy-two 1-day-old Arbor Acres (AA) broilers were randomly divided into 4 groups with 3 replicates per group and 6 chickens per replicate. The control group (group CK) was fed a basal diet, the antibiotic group (group K) was fed the basal diet supplemented with 250 mg/kg chlortetracycline hydrochloride, the EGF group (group E) was fed the basal diet supplemented with 500 mg/kg EGF, and the EGF+antibiotic group (group E+K) was fed the basal diet supplemented with 500 mg/kg EGF+250 mg/kg chlortetracycline hydrochloride. The test period was 42 days. The hematoxylin-eosin (HE) staining, double antibody sandwich and in situ hybridization methods were used to detect the duodenal mucosa morphology, the activities of alkaline protease, lipase and α-amylase and the expression of EGFR. The results showed as follows:1) at 28, 35 and 42 days of age, there was no significant difference in duodenal alkaline protease activity among all group (P>0.05), but the duodenal alkaline protease activity in group E was the highest. At 21, 28, 35 and 42 days of age, there was no significant difference in duodenal lipase activity among all group (P>0.05), but the duodenal lipase activity in group E was the highest. At 35 days of age, the duodenal α-amylase activity in group E was significantly higher than that in group CK and group E+K (P<0.05); at 42 days of age, the duodenal α-amylase activity in group E was significantly higher than that in group K and group E+K (P<0.01). 2) At 21 days of age, the duodenal EGFR gene average optical density in group E was significantly higher than that of group CK (P<0.05); at 42 days of age, the duodenal EGFR gene average optical density in group E and group K was significantly higher than that in group CK and group E+K (P<0.01). 3) At 21 days of age, the duodenal villi height and villus height/crypt depth in group K and group E were significantly higher than those in group CK (P<0.05). At 28, 35 and 42 days of age, there were no significant differences in the duodenal villi height, crypt depth, and villus height/crypt depth among all groups (P>0.05). At 21, 35 and 42 days of age, the villus height/crypt depth in group E was the highest. In summary, diet adding 500 mg/kg EGF can promote the development of the duodenal mucosa morphology of broilers at 21, 28, 35 and 42 days of age, and enhance the activities of alkaline protease, lipase and α-starch in duodenum, improve the duodenal EGFR expression.

Key words： epidermal growth factor; broilers; duodenum; morphology; digestive enzyme activities

Cite this article

SHAN Chunqiao , LIU Qiuchen , LI Juan , LIU En , LU Jishuang , LIU Yan . Effects of Epidermal Growth Factor on Duodenal Morphology, Digestive Enzyme Activities and Epidermal Growth Factor Receptor Expression of Broilers[J]. Chinese Journal of Animal Nutrition, 2021 , 33(6) : 3218 -3227 . DOI: 10.3969/j.issn.1006-267x.2021.06.024

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