Isolation and Identification of Lactic Acid Bacteria from Sheep Rumen and Analysis of Their Biological Characteristics

HE Jiangbo; YAO Zhifang; WU Guofang; YANG Yuxin; WANG Lei

doi:10.3969/j.issn.1006-267x.2021.06.038

Chinese Journal of Animal Nutrition >

2021 , Vol. 33 >Issue 6: 3365 - 3379

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.06.038

RUMINANT AND HERBIVORE NUTRITION AND FEED

Isolation and Identification of Lactic Acid Bacteria from Sheep Rumen and Analysis of Their Biological Characteristics

HE Jiangbo ,
YAO Zhifang ,
WU Guofang ,
YANG Yuxin ,
WANG Lei

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1. College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China;
2. Academy of Animal Science and Veterinary Medicine, Qinghai University, Xining 810016, China

Received date: 2020-12-02

Online published: 2021-06-10

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Abstract

This study aimed to quest several excellently probiotic lactobacilli which could substitute antibiotics, and provide theoretical support for subsequent application in livestock and poultry breeding. In this study, rumen contents collected from Qinghai black Tibetan sheep and Tibetan white cashmere goats were taken as source bacteria strains. The lactobacilli were isolated and purified using MRS solid medium with CaCO₃. A total of 22 strains of homotypic fermenting lactobacilli were obtained after a series of physiological-biochemical tests, 16S rRNA sequencing identification, and acid and gas productivity tests. Determination and evaluation tests were proceeded with regards to growth rates, acidogenicity, tolerance to acid and bile salt, bacteriostatic performance, self-agglutination rate, as well sensitivity to antibiotics. Five bacterial strains were screened from the species of Pediococcus, Enterococcus, and Lactobacillus after a comparison on biological features of these strains. The screened strains consisted of two stains of Pediococcus pentosaceus, one strain of Enterococcus hirschii, two strains of Lactobacillus pentosus. The results showed that both strains of Pediococcus pentosaceus entered logarithmic growth phase after 4 h of cultivation, and reached stable phase after 16 h; the pH of bacteria solutions went below 4.0 after 24 h and it was observed that both strains could tolerate a pH of 3.0 and a bile salt environment of 0.03%. The only strain of Enterococcus hirschii entered the algorithmic growth phase after 2 h of cultivation, and reached stable phase after 12 h; the pH of bacteria solutions went below 4.5 after 24 h and it was observed that bacterial strain could tolerate a pH of 4.0 and a bile salt environment of 0.3%. The two strains of Lactobacillus pentosus entered the algorithmic growth phase after 2 h of cultivation, and reached stable phase after 16 h; the pH of bacteria solutions went below 4.0 after 16 h and it was observed that bacterial strain could tolerate a pH value of 3.0 and a bile salt environment of 0.3%. This study found that all of the five lactobacilli from different species had excellent self-agglutination rate, bacteriostatic performance, and safety. In conclusion, this 5 strains are believed promising source strains for the follow-up production of micro-organism fermented feeds and micro-ecological preparation products for animals considering their excellent biological characteristics.

Key words： sheep; rumen; lactic acid bacteria; isolation; identification; biological characteristics

Cite this article

HE Jiangbo , YAO Zhifang , WU Guofang , YANG Yuxin , WANG Lei . Isolation and Identification of Lactic Acid Bacteria from Sheep Rumen and Analysis of Their Biological Characteristics[J]. Chinese Journal of Animal Nutrition, 2021 , 33(6) : 3365 -3379 . DOI: 10.3969/j.issn.1006-267x.2021.06.038

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