Effects of Artemisia argyi Essential Oils on Anti-Inflammatory of Macrophages Induced by Lipopolysaccharide

CAO Jinling; CHEN Jianjie; LI Lijuan; CHANG Ouqin; HUANG Zhibin

doi:10.3969/j.issn.1006-267x.2021.06.049

Chinese Journal of Animal Nutrition >

2021 , Vol. 33 >Issue 6: 3479 - 3486

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.06.049

MOLECULAR AND CELLULAR NUTRITION

Effects of Artemisia argyi Essential Oils on Anti-Inflammatory of Macrophages Induced by Lipopolysaccharide

CAO Jinling ,
CHEN Jianjie ,
LI Lijuan ,
CHANG Ouqin ,
HUANG Zhibin

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1. College of Food Science and Engineering, Shanxi Agricultural University, Taigu 030801, China;
2. College of Veterinary Medicine, Shanxi Agricultural University, Taigu 030801, China;
3. College of Information, Shanxi Agricultural University, Taigu 030801, China;
4. Key Laboratory of Aquatic Animal Immune Technology of Guangdong Province, Key Laboratory of Fishery Drug Development, Ministry of Agriculture and Rural Affairs, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, China

Received date: 2020-11-18

Online published: 2021-06-10

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Abstract

This experiment was conducted to study the effects of Artemisia argyi essential oils (AAEO) on anti-inflammatory of macrophages induced by lipopolysaccharide (LPS). The inflammatory model was established using mouse macrophages (RAW264.7 cells) induced by LPS, and set control group[added medium with 0.1% dimethyl sulfoxide (DMSO)], LPS group (added medium with 1 μg/mL LPS) and different concentrations of AAEO groups (added 5, 10 and 20 μg/mL AAEO, respectively, pretreatment for 1 h, and added medium with 1 μg/mL LPS), each group was set with 3 replicates and cultured for 24 h. The results showed that compared with the control group, the contents of nitric oxide (NO), tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), interleukin-6 (IL-6) and monocyte chemoattractant protein-1 (MCP-1) and mRNA relative expression levels of TNF-α, IL-1, IL-6 and inducible nitric oxide synthase (iNOS) of RAW264.7 cells of LPS group were significantly increased (P<0.05). Compared with the LPS group, the contents of NO, TNF-α, IL-6 and MCP-1 and mRNA relative expression levels of IL-6 and iNOS of RAW264.7 cells of 5, 10 and 20 μg/mL AAEO groups were significantly decreased (P<0.05), and the IL-1β content and IL-1β mRNA relative expression level of RAW264.7 cells of 10 and 20 μg/mL AAEO groups were significantly decreased (P<0.05). Above all, AAEO can alleviate LPS-induced inflammation of macrophage by regulating the mRNA expression of inflammatory cytokines and the secretion of cytokines and inflammatory mediators, and there is a certain dose-response relationship between AAEO concentration and these indexes. AAEO has certain anti-inflammatory activity.

Key words： Artemisia argyi essential oils; lipopolysaccharide; RAW264.7 cells; inflammation

Cite this article

CAO Jinling , CHEN Jianjie , LI Lijuan , CHANG Ouqin , HUANG Zhibin . Effects of Artemisia argyi Essential Oils on Anti-Inflammatory of Macrophages Induced by Lipopolysaccharide[J]. Chinese Journal of Animal Nutrition, 2021 , 33(6) : 3479 -3486 . DOI: 10.3969/j.issn.1006-267x.2021.06.049

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