Effects of High Temperature on Apoptosis and Fat Deposition of 3T3-L1 Preadipocytes

XIE Hongyue; PAN Peng; HU Yan; ZHANG Yu; LUO Yunyan; HU Xuxu; ZHEN Rui; JIANG Qinyang; HUANG Yanna

doi:10.3969/j.issn.1006-267x.2021.06.050

Chinese Journal of Animal Nutrition >

2021 , Vol. 33 >Issue 6: 3487 - 3496

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.06.050

MOLECULAR AND CELLULAR NUTRITION

Effects of High Temperature on Apoptosis and Fat Deposition of 3T3-L1 Preadipocytes

XIE Hongyue ,
PAN Peng ,
HU Yan ,
ZHANG Yu ,
LUO Yunyan ,
HU Xuxu ,
ZHEN Rui ,
JIANG Qinyang ,
HUANG Yanna

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College of Animal Science and Technology, Guangxi University, Nanning 530004, China

Received date: 2020-11-16

Online published: 2021-06-10

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Abstract

The aim of this study was to investigate the effects of high temperature on the apoptosis and fat deposition of 3T3-L1 preadipocytes. 3T3-L1 preadipocytes were selected as research materials. The cells were treated at 37.0 and 41.5℃ respectively, apoptosis was observed 4 days later, and the number of lipid droplets was observed by oil red O staining after 6 days of differentiation. The mRNA expression levels of heat shock protein family genes involved heat shock protein 60 (HSP60), heat shock protein 70 (HSP70), heat shock protein 90 (HSP90), apoptosis related genes involved cysteine aspartate protease-3 (Caspase-3) and cysteine aspartate protease-9 (Caspase-9), B lymphocytoma-2 (Bcl-2) and Bcl-2-associated X protein (Bax), fat synthesis related genes involved peroxisome proliferators-activated receptor γ (PPARγ), fatty acid synthase (FAS), acetyl CoA carboxylase (ACC), fatty acid-binding protein 2 (AP2), CCAAT/enhancer-binding proteinα (CEBPα), and fat decomposition related genes involved hormone-sensitive triglayceride lipase (HSL) and adipose triglyceride lipase (ATGL) were determined by real-time quantitative PCR (qRT-PCR). The results showed that the mRNA expression levels of HSP60, HSP70 and HSP90 were extremely significantly up-regulated after high temperature treatment (P<0.01 or P<0.001). The mRNA expression levels of Caspase-3 and Caspase-9 were extremely significantly up-regulated (P<0.01), while the Bcl-2/Bax mRNA ratio was extremely significantly decreased (P<0.001). A large number of intracellular lipid droplets were clustered, and the staining of lipid droplets with oil red O staining was obvious. Further studies showed that the mRNA expression levels of PPARγ, FAS, ACC, AP2 and CEBPα were also extremely significantly up-regulated (P<0.01 or P<0.001), while the mRNA expression levels of ATGL and HSL were extremely significantly down-regulated (P<0.001). In conclusion, high temperature can induce apoptosis of 3T3-L1 preadipocytes, promote the synthesis of fatty acids, inhibit the decomposition of fatty acids, and then increase the lipid accumulation of 3T3-L1 preadipocytes.

Key words： high temperature; 3T3-L1 preadipocytes; apoptosis; fat synthesis; fat decomposition

Cite this article

XIE Hongyue , PAN Peng , HU Yan , ZHANG Yu , LUO Yunyan , HU Xuxu , ZHEN Rui , JIANG Qinyang , HUANG Yanna . Effects of High Temperature on Apoptosis and Fat Deposition of 3T3-L1 Preadipocytes[J]. Chinese Journal of Animal Nutrition, 2021 , 33(6) : 3487 -3496 . DOI: 10.3969/j.issn.1006-267x.2021.06.050

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