EXPERIMENTAL METHOD AND ANIMAL

Comparison of Rumen Fermentation Characteristics and Methane Production of Different Distillers Grains

  • DAI Qindan ,
  • WANG Zhisheng ,
  • HU Rui ,
  • ZHOU Xinyu ,
  • ZHU Yixiao ,
  • WANG Cheng ,
  • WANG Xueying ,
  • ZOU Huawei ,
  • SHI Junhua ,
  • PENG Quanhui ,
  • XUE Bai ,
  • WANG Lizhi
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  • Key Laboratory of Low Carbon Breeding and Safe Production of Cattle, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu 611130, China

Received date: 2020-11-18

  Online published: 2021-06-10

Abstract

The purpose of this study was to evaluate the rumen fermentation characteristics and methane production of different types of distiller's grains by in vitro gas production method. In this study, Luzhou-flavor distillers' grains, Maotai-flavor distillers' grains, highland barley grains, Maotai-flavor fermented distillers' grains, distillers dried grains with solubles and beer distillers' grains were selected as the experimental objects to perform the in vitro fermentation experiments. The microbial protein content, ammonia nitrogen, volatile fatty acids concentration, methane, hydrogen and carbon dioxide production in the fermentation broth were measured. Additionally, the correlation between volatile fatty acids concentration and methane production and nutritional components was analyzed. The results showed as follows:1) the cumulative gas production, theoretical maximum gas production, propionate/total volatile fatty acid and carbon dioxide production of highland barley grains and distillers dried grains with solubles after fermentation 72 h in vitro fermentation were significantly higher than those of other distillers' grains (P<0.05). The cumulative gas production, theoretical maximum gas production and total volatile fatty acid concentration of Maotai-flavor fermented distillers' grains were significantly lower than those of other distillers' grains (P<0.05). The concentration of ammonia nitrogen of beer distillers' grains and highland barley grains was significantly higher than that of other distiller's grains (P<0.05), and the microbial protein content of distillers dried grains with solubles was significantly higher than that of other distiller's grains (P<0.05). The methane production of highland barley grains was the highest after fermentation 72 h in vitro fermentation, followed by distillers dried grains with solubles and beer distillers' grains, while the methane production of Maotai-flavor distillers' grains and Luzhou-flavor distillers' grains was significantly lower than that of other distiller's grains (P<0.05). 2) The neutral detergent soluble matter content was positively correlated with total volatile fatty acid concentration and methane production (P<0.05). On the contrary, neutral detergent fiber and acid detergent fiber contents were negatively correlated with total volatile fatty acid concentration and methane production (P<0.05). Therefore, due to the high degradable carbohydrates, the rumen fermentation effect of highland barley grains and distillers dried grains with solubles are better than other distillers' grains. However, the highland barley grains and distillers dried grains with solubles display high methane production, which is not conducive to emission reduction. The methane production of Maotai-flavor fermented distillers' grains is the lowest. Furthermore, the neutral detergent soluble matter content show positive correlation with total volatile fatty acid concentration and methane production.

Cite this article

DAI Qindan , WANG Zhisheng , HU Rui , ZHOU Xinyu , ZHU Yixiao , WANG Cheng , WANG Xueying , ZOU Huawei , SHI Junhua , PENG Quanhui , XUE Bai , WANG Lizhi . Comparison of Rumen Fermentation Characteristics and Methane Production of Different Distillers Grains[J]. Chinese Journal of Animal Nutrition, 2021 , 33(6) : 3497 -3506 . DOI: 10.3969/j.issn.1006-267x.2021.06.051

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