EXPERIMENTAL METHOD AND ANIMAL

Effects of Mannose Oligosaccharide on Repair of Intestinal Injury Induced by Aspirin in Rats

  • CHEN Yongjiang ,
  • WANG Jinrong ,
  • QIAO Hanzhen ,
  • SU Lanli ,
  • TANG Guifen ,
  • HUANG Jin ,
  • ZHAO Yinli ,
  • GAO Wenting
Expand
  • 1. College of Bioengineering, Henan University of Technology, Zhengzhou 450001, China;
    2. Henan University of Animal Husbandry and Economy, Zhengzhou 450011, China

Received date: 2020-11-13

  Online published: 2021-06-10

Abstract

The objective of the experiment was to investigate the therapeutic effect of mannose oligosaccharides (MOS) on repairmen of aspirin (ASA) induced intestinal injury in rats. The experiment was designed by single factor test, 36 rats at 6 weeks of age with body weight of 150 to 180 g were randomly divided into 6 groups after 1 week adaptive feeding, each group had 6 rats which fed in a single cage. The model group was administered orally 200 mg/kg ASA for 2 weeks then saline for 1 week. The MOS group was administered orally 600 mg/kg MOS. The ASA1, ASA2 and ASA3 groups were administered orally 50, 100 and 200 mg/kg ASA, respectively, then 600 mg/kg MOS for the next week. The blank group received the same amount of normal saline. The results showed that compared with the blank group, there was no significant difference in interleukin-2 (IL-2) content, lysozyme (LZM) and total superoxide dismutase (T-SOD) activities and intestinal crypt depth (CD) in ASA1 group (P>0.05), no significant difference in IL-2 content and CD in ASA2 group and control group (P>0.05) and ASA3 group was higher than model group (P<0.05), the ratio of villi height to crypt depth (VH/CD) was higher than that in model group (P<0.05), and there was no difference from the blank group (P>0.05). Compared with blank group, the content of mucosal secretarial immunoglobulin A (sIgA) in model group was decreased by 30.32% (P<0.05), but there was no significant difference from ASA1 group (P>0.05), and it was decreased by 7.39% and 12.50% in ASA2 and ASA3 groups, respectively (P<0.05). Compared with model group, it was increased by 25.59% in ASA3 group (P<0.05). The present study is demonstrated that 600 mg/kg MOS administered orally to the rats with intestinal injury which induced by low concentration of ASA can improve the rats intestinal morphology, increase intestinal CD and VH/CD, and increase the serum IL-2 content and LZM activity and sIgA content in rats in ASA1 group, MOS can promote intestinal development and increase serum T-SOD activity in the ASA3 group compared with the model group.

Cite this article

CHEN Yongjiang , WANG Jinrong , QIAO Hanzhen , SU Lanli , TANG Guifen , HUANG Jin , ZHAO Yinli , GAO Wenting . Effects of Mannose Oligosaccharide on Repair of Intestinal Injury Induced by Aspirin in Rats[J]. Chinese Journal of Animal Nutrition, 2021 , 33(6) : 3523 -3531 . DOI: 10.3969/j.issn.1006-267x.2021.06.054

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