SHORT COMMUNICATION

Effects of Monoammonium Glycyrrhizinate on Rumen Fermentation Parameters and Methane Production in Vitro

  • JIANG Chenyu ,
  • CUI Haoran ,
  • BAI Tiantian ,
  • PU Xuanxuan ,
  • LIU Junfeng ,
  • GUO Xuefeng
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  • 1. College of Animal Science, Tarim University, Alar 843300, China;
    2. Key Laboratory of Animal Science and Technology Corps, Alar 843300, China

Received date: 2020-11-10

  Online published: 2021-06-10

Abstract

The purpose of this experiment was to investigate the effects of different concentrations of monoammonium glycyrrhizinate on rumen fermentation parameters and methane production in vitro and to select the most appropriate concentration. In this experiment, three sheep with permanent fistulas were selected as rumen fluid donor sheep, and the basic diet which supplemented the monoammonium glycyrrhizinate as the fermentation substrate. The experiments were divided into control group, Ⅰ group, Ⅱ group, Ⅲ group, Ⅳ group and Ⅴ group, which were added 0, 50, 150, 250, 350 and 450 mg/L of monoammonium glycyrrhizinate, respectively, and cultivated for 3, 9, 12, 21 and 24 h, respectively, and pH, ammoniacal nitrogen (NH3-N) concentration, gas production, dry matter degradation rate, volatile fatty acid (VFA) concentration and methane production of fermentation fluid were measured. The experiment results showed that after 24 h of fermentation, the gas production of Ⅲ group, Ⅳ group and V group were significantly lower than those of the control group (P<0.05); the pH of each group increased with fermentation time and showed a downward trend; the dry matter degradation rate increased with fermentation time extended, but the differences between the experimental groups and the control group were not significant (P>0.05); the NH3-N concentration of the groups decreased with the increase of monoammonium glycyrrhizinate concentration, and the NH3-N concentration of Ⅲ group, Ⅳ group and Ⅴ group was significantly lower than that of the control group (P<0.05); compared with the control group, acetic acid, propionic acid and butyric acid concentrations in all the groups had a downward trend, but the difference were not significant (P>0.05); after 24 h of fermentation, the methane production of each group was decreased, and the methane production of Ⅳ group was the lowest, but the difference was not significant compared with the control group (P>0.05). According to a comprehensive evaluation of multiple indices, the suitable concentration of monoammonium glycyrrhizinate is 350 mg/L in vitro for sheep.

Cite this article

JIANG Chenyu , CUI Haoran , BAI Tiantian , PU Xuanxuan , LIU Junfeng , GUO Xuefeng . Effects of Monoammonium Glycyrrhizinate on Rumen Fermentation Parameters and Methane Production in Vitro[J]. Chinese Journal of Animal Nutrition, 2021 , 33(6) : 3554 -3563 . DOI: 10.3969/j.issn.1006-267x.2021.06.057

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