Chinese Journal of Animal Nutrition >

2021 , Vol. 33 >Issue 6: 3581 - 3591

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.06.060

SHORT COMMUNICATION

Consistency and Additivity in Gross Energy of Monogastric Animal Feed Samples between Different Laboratories

  • ZHANG Jinyuan ,
  • WANG Yuming ,
  • DU Zhongyuan ,
  • DONG Ying ,
  • LI Dailin ,
  • WANG Yueli ,
  • HUANG Qinghua ,
  • DU Qingzhi ,
  • HUANG Yanling ,
  • ZHAO Feng
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  • 1. College of Animal and Veterinary Science, Southwest Minzu University, Chengdu 610041, China;
    2. State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
    3. Wenshi Food Group Limited Company, Yunfu 527400, China;
    4. New Hope Liuhe Limited Company, Beijing 100102, China;
    5. Tangrenshen Group Limited Company, Zhuzhou 412000, China;
    6. Qingdao Weilan Biology Limited Company, Qingdao 266000, China;
    7. Hunan Zhongben Intelligent Technology Development Limited Company, Changsha 410013, China

Received date: 2020-11-24

  Online published: 2021-06-10

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Abstract

The objective of this study was to compare the variation and additivity of gross energy (GE) measured in different laboratories, which will provide a reference for accurately determining effective energy value of feed. This experiment consisted of two parts. Part 1 was to investigate the effects of the feed sample volumes on the GE value determined by the oxygen bomb calorimeter. A single factor completely random design for sample volumes at 0.2, 0.4, 0.6, 0.8 and 1.0 g was adopted for each of 6 feed ingredients (corn, soybean meal, wheat bran, soybean oil, rapeseed meal and cottonseed meal) and 10 diets. Each treatment contained 4 replicates. Part 2 was to investigate the variation and additivity of GE in feed determined in different laboratories. A single factor completely random design for 6 laborotaries was adopted for each feed. The GE of each sample was measured for 4 replicates in each laboratory. The results showed that the measured GE of 6 feed ingredients and 10 diets increased linearly and quadraticly with the increment of sample volumes (P<0.05). The measured GE values at sample volume of 0.2 g were lower than the GE values measured at sample volume of 0.4 to 1.0 g (P<0.05). Relatively little difference was observed in GE measured at sample volumes of 0.6 to 1.0 g. In the comparison among 6 laboratories, the absoute Z scores of 91 out of 96 measured GE were less than 2. The h statistics showed that the measured GE in laboratory 2 was lower, whereas the measured GE in laboratory 5 was higher than other laboratories. The k statistics showed that the variation of GE measured in laboratory 5 and 6 was relatively greater. In total, the coefficient of variation for repeatability, inter-laboratory and reproducibility of GE didn't exceed 0.64%. The paired t test showed that no significant differences between the calculated and measured GE of 10 diets were observed in each of laboratory 1, 3, 5 and 6. Whereas, significant differences between the calculated and measured GE of 10 diets were observed in each of laboratory 2 and 4 (P<0.05). The linear regression model of measured on calculated GE in each of 6 laboratories all overlaped with Y=X. This indicates that the loading volumes of sample for GE measurement using the oxygen bomb calorimeter should be more than 0.6 g. The GE value of feed measured in 6 laboratories show good consistency (94.8% satisfaction), and low coefficient of variation is observed in GE measured among laboratories. Higher and high additivity of dietary GE are present in laboratory 4 and 2, respectively.

Key words： feed; gross energy; coefficient of variation; additivity

Cite this article

ZHANG Jinyuan , WANG Yuming , DU Zhongyuan , DONG Ying , LI Dailin , WANG Yueli , HUANG Qinghua , DU Qingzhi , HUANG Yanling , ZHAO Feng . Consistency and Additivity in Gross Energy of Monogastric Animal Feed Samples between Different Laboratories[J]. Chinese Journal of Animal Nutrition, 2021 , 33(6) : 3581 -3591 . DOI: 10.3969/j.issn.1006-267x.2021.06.060

References

[1] 中华人民共和国国家质量监督检验检疫总局,中国国家标准化管理委员会.GB/T 213-2008煤的发热量测定方法[S].北京:中国标准出版社,2009. General Administration of Quality Supervision,Inspection and Quarantine of the People's Republic of China,Standardization Administration of China.GB/T 213-2008 Determination of calorific value of coal[S].Beijing:Standards Press of China,2008.(in Chinese)
[2] International Organization for Standardization.ISO 9831:1998 Animal feeding stuffs,animal products,and faeces or urine-determination of gross calorific value-Bomb calorimeter method[S].Switzerland:International Organization for Standardization,1998.
[3] BOURDILLON A,CARRÉ B,CONAN L,et al.European reference method of in vivo determination of metabolisable energy in poultry:reproducibility,effect of age,comparison with predicted values[J].British Poultry Science,1990,31(3):567-576.  
[4] BOURDILLON A,CARRÉ B,CONAN L,et al.European reference method for the in vivo determination of metabolisable energy with adult cockerels:reproducibility,effect of food intake and comparison with individual laboratory methods[J].British Poultry Science,1990,31(3):557-565.  
[5] PÉREZ J M,CERVERA C,CUNHA L F E,et al.European ring-test on in vivo determination of digestibility in rabbits:reproducibility of a reference method in comparison with domestic laboratory procedures[J].World Rabbit Science,1995,3(4):171-178.
[6] SIBBALD I R,MORSE P M.Variation among gross energy values measured by two modes of adiabatic oxygen bomb calorimetry[J].Poultry Science,1982,61(5):994-997.  
[7] 杜中原,苏艳芳,陈凯旋,等.2种氧弹热量计测定样品总能的差异及其对能量消化率的影响[J].动物营养学报,2021,33(2):1128-1136. DU Z Y,SU Y F,CHEN K X,et al.Difference in two types of oxygen bomb calorimeters to determine gross energy and its effect on energy digestibility of samples[J].Chinese Journal of Animal Nutrition,2021,33(2):1128-1136.(in Chinese)
[8] 中国农业科学院北京畜牧兽医研究所,中国饲料数据库情报网中心,动物营养学国家重点实验室.中国饲料成分及营养价值表(2019年第30版)制订说明[J].中国饲料,2019(21):97-107. Institute of Animal Sciences of CAAS,Chinese Feed Database Information Center,State Key Laboratory of Animal Nutrition.Introduction of tables of feed composition and nutritive values in China(2019 thirty edition)[J].China Feed,2019(21):97-107. (in Chinese)
[9] 王亚,赵峰,张虎,等.单宁酸对生长猪胃-小肠仿生消化中消化酶活性及饲粮粗蛋白消化率的影响[J].畜牧兽医学报,2020,51(6):1332-1341. WANG Y,ZHAO F,ZHANG H,et al.Effects of tannic acid on digestive enzyme activity and digestibility of dietary crude protein in simulated gastric and intestinal digestion for growing pigs[J].Chinese Journal of Animal and Veterinary Sciences,2020,51(6):1332-1341. (in Chinese)
[10] 中华人民共和国国家质量监督检验检疫总局,中国国家标准化管理委员会.GB/T 6435-2014饲料中水分的测定[S].北京:中国标准出版社,2015. General Administration of Quality Supervision,Inspection and Quarantine of the People's Republic of China,Standardization Administration of China.GB/T 6435-2014 Determination of moisture in feedstuffs[S].Beijing:Standards Press of China,2014. (in Chinese)
[11] 国家市场监督管理总局,国家标准化管理委员会.GB/T 6432-2018饲料中粗蛋白的测定凯氏定氮法[S].北京:中国标准出版社,2018. General Administration of Quality Supervision,Inspection and Quarantine of the People's Republic of China,Standardization Administration of China.GB/T 6432-2018 Determination of crude protein in feeds-Kjeldahl method[S].Beijing:Standards Press of China,2018. (in Chinese)
[12] 中华人民共和国国家质量监督检验检疫总局,中国国家标准化管理委员会.GB/T 6433-2006饲料中粗脂肪的测定[S].北京:中国标准出版社,2006. General Administration of Quality Supervision,Inspection and Quarantine of the People's Republic of China,Standardization Administration of China.GB/T 6433-2006 Determination of crude fat in feeds[S].Beijing:Standards Press of China,2006. (in Chinese)
[13] 中华人民共和国国家质量监督检验检疫总局,中国国家标准化管理委员会.GB/T 6438-2007饲料中粗灰分的测定[S].北京:中国标准出版社,2007. General Administration of Quality Supervision,Inspection and Quarantine of the People's Republic of China,Standardization Administration of China.GB/T 6438-2007 Animal feeding stuffs-Determination of crude ash[S].Beijing:Standards Press of China,2007. (in Chinese)
[14] 中华人民共和国国家市场监督管理总局,中国国家标准化管理委员会.GB/T 28043-2019利用实验室间比对进行能力验证的统计方法[S].北京:中国标准出版社,2019. State Administration for Market Regulation,Standardization Administration of China.GB/T 28043-2019 Statistical methods for use in proficiency testing by interlaboratory comparison[S].Beijing:Standards Press of China,2019. (in Chinese)
[15] 吴旻.内部比对试验结果的统计评价方法[J].理化检验(化学分册),2020,56(7):777-781. WU M.Statistics and evaluation methods of results of inter comparison tests[J].Physical Testing and Chemical Analysis (Chemical Analysis),2020,56(7):777-781. (in Chinese)
[16] 中华人民共和国国家质量监督检验检疫总局,中国国家标准化管理委员会.GB/T 6379.2-2004测量方法与结果的准确度(正确度与精密度)第2部分:确定标准测量方法重复性与再现性的基本方法[S].北京:中国标准出版社,2005. General Administration of Quality Supervision,Inspection and Quarantine of the People's Republic of China,Standardization Administration of China.Accuracy (trueness and precision) of measurement methods and results-Part 2:basic method for the determination of repeatability and reproducibility of a standard measurement method[S].Beijing:Standards Press of China,2005. (in Chinese)
[17] SHEN J F,ZHU S G,LIU X Z,et al.Measurement of heating value of rice husk by using oxygen bomb calorimeter with benzoic acid as combustion adjuvant[J].Energy Procedia,2012,17:208-213.
[18] WEI J,XIE M,TANG J,et al.The feasibility of enzyme hydrolysate gross energy for formulating duck feeds[J].Poultry Science,2020,99(8):3941-3947.  
[19] 马艳,谭卫红,沈娟章,等.新型氧弹量热仪测定谷物的热值[J].生命科学仪器,2013,11(3):42-44. MA Y,TAN W H,SHEN J Z,et al.Determination of the heat values of several cereals with bomb-calorimeter[J].Life Science Instruments,2013,11(3):42-44. (in Chinese)
[20] DALE N M,FULLER H L.Additivity of true metabolizable energy values as measured with roosters,broiler chicks,and poults[J].Poultry Science,1980,59(8):1941-1942.  
[21] ZHAO F,REN L Q,MI B M,et al.Developing a computer-controlled simulated digestion system to predict the concentration of metabolizable energy of feedstuffs for rooster[J].Journal of Animal Science,2014,92(4):1537-1547.  
[22] COWIESON A,SORBARA J O,PAPPENBERGER G,et al.Additivity of apparent and standardized ileal amino acid digestibility of corn and soybean meal in broiler diets[J].Poultry Science,2019,98(9):3722-3728.  
[23] WANG T T,OSHO S O,ADEOLA O.Additivity of apparent and standardized ileal digestibility of amino acid determined by chromic oxide and titanium dioxide in mixed diets containing wheat and multiple protein sources fed to growing pigs[J].Journal of Animal Science,2018,96(11):4731-4742.  
[24] AIR ELLEN L,BENOIT S C,CLEGG D J,et al.Insulin and leptin combine additively to reduce food intake and body weight in rats[J].Endocrinology,2002,143(6):2449-2452.  
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